Lucia Tarantino

University of Bologna, Bologna, Emilia-Romagna, Italy

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Publications (10)10.92 Total impact

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    ABSTRACT: The inflammatory myopathies (IMs) are a group of disorders characterised by weakness and inflammation of the skeletal muscles. Muscle biopsy is the most crucial test to confirm the clinical diagnosis, but also the most common cause of misdiagnosis. There are currently no markers specific or sensitive enough to distinguish IMs from other diseases with similar clinical and morphological features, and an international multidisciplinary effort is under way to develop new classification criteria for IMs. Standards for Reporting of Diagnostic Accuracy recommendations to validate a diagnostic test based on the quantification of internal major histocompatibility complex class I (MHC-I) positive fibres were adopted. MHC-I immunostained specimens from 64 patients were scored by two independent blinded investigators, and the percentage of positive fibres was determined. Agreement between investigators was evaluated with the k-weighted statistic. The receiver operating characteristic curve, area under the curve, sensitivity, specificity, and positive and negative predictive values of each percentage range of positive fibres versus the diagnosis of IM were calculated. The main difference between IM and non-inflammatory samples was the number of internal MHC-I positive fibres. The k-weighted value was 0.89 for a percentage of MHC-I positive fibres above 50%; the positive predictive value was 100%, and the negative predictive value was 94%. This is the first study on the validity of a quantitative analysis of internal MHC-I positive fibres for an IM diagnosis performed according to Standards for Reporting of Diagnostic Accuracy recommendations. The interobserver agreement was almost perfect, thus making the method reproducible. Applying an MHC-I cut-off above 50% is an optimal marker for polymyositis (PM) and dermatomyositis (DM) diagnosis.
    Journal of clinical pathology 11/2011; 65(1):14-9. · 2.43 Impact Factor
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    ABSTRACT: Acute kidney injury (AKI) is a major health care condition with limited current treatment options. Within this context, stem cells may provide a clinical approach for AKI. Moreover, a synthetic compound previously developed, hyaluronan monoesters with butyric acid (HB), able to induce metanephric differentiation, formation of capillary-like structures, and secretion of angiogenic cytokines, was tested in vitro. Thereafter, we investigated the effects of human mesenchymal stem cells from fetal membranes (FMhMSCs), both treated and untreated with HB, after induction of ischemic AKI in a rat model. At reperfusion following 45-min clamping of renal pedicles, each rat was randomly assigned to one of four groups: CTR, PBS, MSC, and MSC-HB. Renal function at 1, 3, 5, and 7 days was assessed. Histological samples were analyzed by light and electron microscopy and renal injury was graded. Cytokine analysis on serum samples was performed. FMhMSCs induced an accelerated renal functional recovery, demonstrated by biochemical parameters and confirmed by histology showing that histopathological alterations associated with ischemic injury were less severe in cell-treated kidneys. HB-treated rats showed a minor degree of inflammation, both at cytokine and TEM analyses. Better functional and morphological recovery were not associated to stem cells' regenerative processes, but possibly suggest paracrine effects on microenvironment that induce retrieval of renal damaged tissues. These results suggest that FMhMSCs could be useful in the treatment of AKI and the utilization of synthetic compounds could enhance the recovery induction ability of cells.
    Cell Transplantation 11/2010; 20(8):1193-208. · 4.42 Impact Factor
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    ABSTRACT: Storage diseases (SDs) are rare metabolic disorders characterized by the intra- or extralysosomal accumulation of unmetabolized compounds. Different causes determine the buildup of undigested material, resulting in typical histochemical and ultrastructural changes. Ultrastructural examination of tissue from patients with clinically suspected SDs may disclose pathognomonic alterations or suggest a differential diagnosis even in the absence of clinically evident involvement of the biopsied tissue. Accurate diagnosis of SDs requires a continuous integration of clinical, biochemical, ultrastructural, and, when available, molecular data. It is also important for the pathologist to be familiar with the morphological variability characterizing each SD, because some morphologies are often the early stages of undeveloped forms and morphologically similar diseases are easily confused. The major advantages of transmission electron microscopy (TEM) techniques are discussed, emphasizing the current role of TEM as a rapid, cost-effective, and efficient diagnostic tool.
    Ultrastructural Pathology 10/2010; 34(5):243-51. · 0.98 Impact Factor
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    ABSTRACT: Satellite cells (SCs) are skeletal muscle progenitor cells located between the basal lamina and the sarcolemma of muscle fibers. They are responsible for muscle growth and repair. In humans, aging results in the depletion of the SC population and in its proliferative activity, but not in its function. It has not yet been determined whether under conditions of massive muscle fiber death in vivo, the regenerative potential of SCs is totally or partially compromised in old muscle. No studies have yet tested whether advanced age is a factor that restrains the response of SCs to muscle denervation in humans; this is also due to difficulties in the isolation and in the culture of SCs from a small human surgery fragment. The aim of this study was to study in depth muscle regeneration analysing the SC ability of SCs to proliferate and differentiate in aging human patients. In order to study in more detail the molecular mechanism, the proliferative and differentiative ability of aging SCs, we isolated SCs from aging human muscle biopsies and analysed their morphology by transmission electron microscopy and immunocytochemical analysis (antibodies against desmin, N-CAM and M-cadherin) and their capacity to grow and to expand in vitro. Moreover, in order to evaluate gene expression of myogenic regulatory factors Myf5, MyoD and myogenin (Myf4), RT-PCR was performed. SCs isolated from aging human muscle biopsies and plated into favorable proliferation and differentiation conditions were able to proceed through the myogenic program and actively form myotubes, although taking longer than the young control sample. The RT-PCR analysis together with the ultrastructural SC features showed that the myogenic potential seemed to be compromised during the aging human muscle proliferation in vitro.
    Neurological Research 02/2010; 32(1):63-72. · 1.18 Impact Factor
  • Neuromuscular Disorders - NEUROMUSCULAR DISORD. 01/2009; 19(8):652-652.
  • Neuromuscular Disorders - NEUROMUSCULAR DISORD. 01/2009; 19(8):566-567.
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    ABSTRACT: A 66-year-old woman was referred with left hearing loss. A probable diagnosis of left secretory otitis media with effusion was formulated. A left myringotomy was performed to remove hyperplastic hard tissue from the tympanic cavity. A high resolution CT scan of the temporal bone disclosed a soft-tissue mass completely involving the mastoid and tympanic cavity, surrounding the ossicular chain which appeared spared with no signs of infiltration. The histopathologic, immunohistochemical and ultrastructural response was secretory meningioma, a rare variant of conventional meningothelial meningioma in atypical sites.
    Neuropathology 03/2008; 28(1):69-73. · 1.91 Impact Factor
  • Neuromuscular Disorders - NEUROMUSCULAR DISORD. 01/2007; 17(9):771-771.
  • Neuromuscular Disorders - NEUROMUSCULAR DISORD. 01/2007; 17(9):872-872.
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    ABSTRACT: Electron microscopy has a strategic position in the diagnosis of neuromuscular disorders. In muscular fibers the main abnormalities includes vacuoles, vesicles, inclusion bodies, sarcoplasmic reticulum modifications and mitochondrial alterations. The differential diagnosis includes polymyositis, inclusion body myositis, some sarcolemmal and non-sarcolemmal dystrophies, lysosomal storage diseases. Degenerative alterations of mitochondria, causing swelling and cristae fragmentation, may be considered also as a precocious feature of drug-induced muscle damage guiding molecular genetic analysis. The ultrastructural approach seems to improve the diagnostic accuracy in muscle disorders, making possible the identification of changes and structures related to new hypothesized pathogenetic mechanisms. Muscle biopsy is an essential component of the investigation of patient with neuromuscular disorder, and is the deciding factor for diagnosis in most instances. Electron microscopy, EM, has a strategic position improving the diagnostic accuracy of numerous muscular diseases, some not revealed by light microscopy [16, 22]. Moreover, it gives insight into pathophysiologic mechanisms and can guide molecular genetic analysis. Muscle disorders can be divided into three groups [22]. The first concerns diseases of the interstitial tissue: inflammatory disorders as well as abnormal deposits such amyloidosis. For the first group, histological and immunohistochemical techniques are more effective than EM. The second group refers to diseases associated with defects of protein components of sarcolemma, extracellular matrix and nuclear membrane: with these diseases EM can be considered a valuable tool confirming diagnosis suggested by immunohistochemical and molecular analysis [16, 22]. Finally, ultrastructural study provides the best results for sarcoplasmic abnormalities including vacuoles, inclusion bodies, and some changes not revealed by light microscopy [16, 22]. Materials and Methods To define the relationship between molecular mechanisms and submicroscopic alterations we examined at ultrastructural level 45 muscle biopsies: 4 inclusion body myositis, IBM, 1 polymyositis, 2 dermatomyositis, DM, 2 HIV-related myopathy; 6 LGMD1C, 10 LGMD2B, 10 LGMD2I previously diagnosed by IHC, WB and DNA molecular assay; 4 acid maltase deficiency, AMD, 1 Danon disease, 1 ceroid lipofuscinosis, CL, and 4 drug-induced myopathy. Immunocytochemical analysis was performed by immunogold technique in LGMD1C as previously described [33]. Briefly, the specimens were fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1M, post-fixed in 1% osmium tetroxide in the same buffer, dehydrated in ethanol and embedded in Araldite. Thin sections, stained with uranyl acetate and lead citrate were observed under a transmission electron microscope Philips 410.
    Basic and applied myology: BAM 01/2007; 17:167-171.

Publication Stats

31 Citations
10.92 Total Impact Points

Institutions

  • 2007–2011
    • University of Bologna
      • • Department of Experimental, Diagnostic and Specialty Medicine DIMES
      • • Department of Biological, Geological and Environmental Sciences BiGeA
      Bologna, Emilia-Romagna, Italy