Sanjeeb K Sahoo

All India Institute of Medical Sciences Bhubaneswar, Bhubaneswar, Orissa, India

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Publications (74)355.03 Total impact

  • Priyambada Parhi, Sanjeeb Kumar Sahoo
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    ABSTRACT: Nowadays, emerging aspects of cancer therapy involve both diagnostic and therapeutic modules in a single setting. Targeted theranostic nanoplatforms have emerged globally as frontier research for the improvement of cancer therapy. Trastuzumab (Tmab), a humanized monoclonal antibody is now being used to target human epidermal growth factor receptor-2 (HER 2) positive cancer cells. In the present study, we have analysed the imaging and theragnosis potentiality of Tmab functionalized lipid based nanoparticles (NPs) loaded with anticancer drug rapamycin and imaging agent (quantum dots) for targeted cancer therapy and imaging. The therapeutic evaluation of drug loaded NPs were evaluated through various in vitro cellular studies. The results showed enhanced therapeutic efficacy of targeted drug loaded NPs over native drug and unconjugated NPs in HER 2 positive SKBR 3 breast cancer cell line. Moreover, exploration of the therapeutic benefits of rapamycin loaded Tmab conjugated NPs (Tmab-rapa-NPs) at molecular level, revealed augmented down regulation of mTOR signalling pathway thereby, inducing more cell death. Above all, our targeted multifunctional NPs have shown an excellent bio-imaging modality both in 2D monolayer and 3D tumor spheroid model. Thus, we can anticipate that such a multimodal nanotheranostic approach may be a useful tool for better cancer management in future. Copyright © 2015 Elsevier Inc. All rights reserved.
    Journal of Colloid and Interface Science 08/2015; 451. DOI:10.1016/j.jcis.2015.03.049 · 3.55 Impact Factor
  • Mallaredy Vandana, Sanjeeb K. Sahoo
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    ABSTRACT: Combination therapy in cancer is now opted as a potential therapeutic strategy for cancer treatment. However, effective delivery of drugs in combination to the tumor site is marred by low bioavailability and systemic toxicity of individual drugs. Polymer therapeutics is indeed an upcoming approach for the combinational drug delivery in favour of better cancer management. Hence, the objective of our investigation was to develop a dual drug PEGylated system that carries two chemotherapeutic drugs simultaneously for effective treatment of cancer. In this regard, we have synthesized Pem-PEG-Gem, wherein pemetrexed (Pem) and gemcitabine (Gem) are conjugated to a heterobifunctional polyethylene glycol (PEG) polymer for the effective treatment of Non Small Cell Lung Cancer (NSCLC). Our results demonstrate enhanced bioavailability of the individual drugs in Pem-PEG-Gem in comparison to the drugs in their native form. The developed Pem-PEG-Gem showed enhanced cell death with respect to their native counterparts when treated singly or in combination against NSCLC cells. This might be attributed to better cellular internalization through the process of macropinocytosis and synergistic cytotoxic action of Pem-PEG-Gem in NSCLC cells. Hence, we propose the above dual drug based polymer therapeutic approach suitable for better clinical application in the treatment of NSCLC. Copyright © 2015 Elsevier B.V. All rights reserved.
    European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 05/2015; 94. DOI:10.1016/j.ejpb.2015.04.017 · 4.25 Impact Factor
  • Abhalaxmi Singh, Sanjeeb K. Sahoo
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    ABSTRACT: With increasing understanding of stem-cell biology, the use of stem cells in clinical treatment has become more of a reality for a variety of incurable diseases. To achieve the clinical use of stem cells, however, their biological and physiological complexity must be understood in detail. Current conventional techniques used can only reveal some basic aspects of stem cells and not their complex biology. With the advent of micro- and nanoengineering tools it has become possible to develop highly precise and standardized protocols to study stem-cell biology in detail and with high throughput and consistency. This chapter describes the different aspects of the need for nanoengineering tools in studying stem-cell biology and some of the developments of nanoengineering tools in this direction, as well as the potential challenges in this field.
    Stem-Cell Nanoengineering, 01/2015: pages 87-95; , ISBN: 9781118540619
  • Manasi Das, Wei Duan, Sanjeeb K Sahoo
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    ABSTRACT: The promising proposition of multifunctional nanoparticles for cancer diagnostics and therapeutics has inspired the development of theranostic approach for improved cancer therapy. Moreover, active targeting of drug carrier to specific target site is crucial for providing efficient delivery of therapeutics and imaging agents. In this regards, the present study investigates the theranostic capabilities of nutlin-3a loaded poly (lactide-co-glycolide) nanoparticles, functionalized with a targeting ligand (EpCAM aptamer) and an imaging agent (quantum dots) for cancer therapy and bioimaging. A wide spectrum of in vitro analysis (cellular uptake study, cytotoxicity assay, cell cycle and apoptosis analysis, apoptosis associated proteins study) revealed superior therapeutic potentiality of targeted drug loaded NPs over other formulations in EpCAM expressing cells. Moreover, our nanotheranostic system served as a superlative bio-imaging modality both in 2D monolayer culture and tumor spheroid model. Our result suggests that, these aptamer-guided multifunctional NPs may act as indispensable nanotheranostic approach towards cancer therapy.
    Nanomedicine Nanotechnology Biology and Medicine 09/2014; 11(2). DOI:10.1016/j.nano.2014.09.002 · 5.98 Impact Factor
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    ABSTRACT: Over expression of drug efflux transporters such as P-glycoprotein (P-gp) cumulatively leading to Multidrug resistance (MDR) embodies a major hindrance for successful cancer therapy. A paradigm nanomedicinal approach involving an anticancer drug and modulators of drug resistance within one multifunctional nanocarrier-based delivery system represent an ideal modality for the treatment of MDR. In this regards, we have developed a cationic polymeric nanoparticulate system loaded with MDR1-siRNA and Doxorubicin. Results indicated augmented synergistic effect of combinational nanoformulation in overcoming MDR in MCF-7/ADR cells. Therefore, the above regime could be a promising co-delivery system for effective therapy of drug resistant breast cancer.
    International Journal of Pharmaceutics 08/2014; DOI:10.1016/j.ijpharm.2014.08.056 · 3.79 Impact Factor
  • Suphiya Parveen, Sanjeeb K. Sahoo
    European Journal of Pharmacology 03/2014; 727:186. DOI:10.1016/j.ejphar.2014.02.009 · 2.68 Impact Factor
  • Abhalaxmi Singh, Sanjeeb K Sahoo
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    ABSTRACT: Multifunctional nanoplatforms represent a cutting edge tool in biomedical applications as a result of their applicability in the concurrent monitoring of medical treatment. Magnetic nanoparticles (MNPs) have generated great interest in the field of cancer nanotheranostics owing to their intrinsic magnetic property that enables them to be used as contrast agents in magnetic resonance imaging and as a therapeutic system in conjunction with hyperthermia. In addition, the physical properties and biocompatibility of MNPs help them to act as efficient drug carriers for targeted therapeutic regimes. In this review, we have discussed the different theranostic applications of MNPs. Further, we have raised the current challenges associated with the clinical translation of MNPs along with future opportunities in this field.
    Drug discovery today 10/2013; DOI:10.1016/j.drudis.2013.10.005 · 5.96 Impact Factor
  • Fahima Dilnawaz, Sanjeeb Kumar Sahoo
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    ABSTRACT: Glioblastomas (GBMs) are highly lethal primary brain tumors. Treatment of these malignant gliomas remains ineffective as these are extremely resistant to chemotherapeutic applications. Furthermore, combination therapy for cancer treatment is becoming more popular because it generates synergistic anticancer effects, by reducing individual drug-related toxicity and associated side effects. Currently, magnetic nanoparticles (MNPs), based drug delivery system has attracted much more attention owing to its intrinsic magnetic properties and drug loading capacity. In the present study, MNPs based drug delivery approach for co- delivering of potent chemotherapeutic drugs such as Curcumin (herbal drug) and Temozolomide (DNA methylating agent) has been implemented. The dual drug loaded MNPs formulations were evaluated in two dimensional (2-D) monolayer culture and three-dimensional (3-D) tumour spheroid culture of T-98G cells for understanding the therapeutic discrepancy. The dual drug loaded MNPs formulations demonstrated higher cytotoxic effect than single drug loaded MNPs formulations as compared to their corresponding native drugs in 2-D and 3-D culture. The combination index (CI) analysis revealed synergistic mode of action of dual drug loaded MNPs formulations, which was further confirmed by cell death induction assay mediated by acridine orange (AO) / propidium iodide (PI) staining, illustrating higher efficacy of the formulation towards GBM therapy.
    European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 07/2013; DOI:10.1016/j.ejpb.2013.07.013 · 4.25 Impact Factor
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    ABSTRACT: In spite of intense research efforts, pancreatic ductal adenocarcinoma remains one of the most deadly malignancies in the world. We and others have previously identified a subpopulation of pancreatic cancer stem cells within the tumor as a critical therapeutic target and additionally shown that the tumor stroma represents not only a restrictive barrier for successful drug delivery, but also serves as a paracrine niche for cancer stem cells. Therefore, we embarked on a large-scale investigation on the effects of combining chemotherapy, hedgehog pathway inhibition, and mTOR inhibition in a preclinical mouse model of pancreatic cancer. Prospective and randomized testing in a set of almost 200 subcutaneous and orthotopic implanted whole-tissue primary human tumor xenografts. The combined targeting of highly chemoresistant cancer stem cells as well as their more differentiated progenies, together with abrogation of the tumor microenvironment by targeting the stroma and enhancing tissue penetration of the chemotherapeutic agent translated into significantly prolonged survival in preclinical models of human pancreatic cancer. Most pronounced therapeutic effects were observed in gemcitabine-resistant patient-derived tumors. Intriguingly, the proposed triple therapy approach could be further enhanced by using a PEGylated formulation of gemcitabine, which significantly increased its bioavailability and tissue penetration, resulting in a further improved overall outcome. This multimodal therapeutic strategy should be further explored in the clinical setting as its success may eventually improve the poor prognosis of patients with pancreatic ductal adenocarcinoma.
    PLoS ONE 06/2013; 8(6):e66371. DOI:10.1371/journal.pone.0066371 · 3.53 Impact Factor
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    Priyambada Parhi, Chandana Mohanty, Sanjeeb Kumar Sahoo
    Acta biomaterialia 06/2013; 9(6):7078. DOI:10.1016/j.actbio.2013.04.006 · 5.68 Impact Factor
  • Acta Biomaterialia 06/2013; 9(6):7075-7075. DOI:10.1016/j.actbio.2013.02.025 · 5.68 Impact Factor
  • Manasi Das, Sanjeeb K. Sahoo
    Acta Biomaterialia 06/2013; 9(6):7076-7076. DOI:10.1016/j.actbio.2013.02.024 · 5.68 Impact Factor
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    Manasi Das, Sanjeeb K Sahoo
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    ABSTRACT: This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. Due to highly unethical practices, which include serial self plagiarism, data manipulation and falsification of results found across multiple papers in Acta Biomaterialia. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.
    Acta biomaterialia 06/2013; 9(6):7076. DOI:10.1016/j.actbio.2010.08.010 · 5.68 Impact Factor
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    Paromita Kundu, Chandana Mohanty, Sanjeeb K Sahoo
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    ABSTRACT: This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. Due to highly unethical practices, which include serial self plagiarism, data manipulation and falsification of results found across multiple papers in Acta Biomaterialia. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.
    Acta biomaterialia 06/2013; 9(6):7074. DOI:10.1016/j.actbio.2012.03.048 · 5.68 Impact Factor
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    ABSTRACT: This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. Due to highly unethical practices, which include serial self plagiarism, data manipulation and falsification of results found across multiple papers in Acta Biomaterialia. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.
    Acta biomaterialia 06/2013; 9(6):7075. DOI:10.1016/j.actbio.2010.02.010 · 5.68 Impact Factor
  • Paromita Kundu, Chandana Mohanty, Sanjeeb K. Sahoo
    Acta Biomaterialia 06/2013; 9(6):7074-7074. DOI:10.1016/j.actbio.2013.02.022 · 5.68 Impact Factor
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    Fahima Dilnawaz, Abhalaxmi Singh, Sanjeeb Kumar Sahoo
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    ABSTRACT: This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. Due to highly unethical practices, which include serial self plagiarism, data manipulation and falsification of results found across multiple papers in Acta Biomaterialia. One of the conditions of submission of a paper for publication is that authors declare explicitly that their work is original and has not appeared in a publication elsewhere. Re-use of any data should be appropriately cited. As such this article represents a severe abuse of the scientific publishing system. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.
    Acta biomaterialia 06/2013; 9(6):7077. DOI:10.1016/j.actbio.2011.10.022 · 5.68 Impact Factor
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    Nanomedicine Nanotechnology Biology and Medicine 05/2013; 9(4):580. DOI:10.1016/j.nano.2013.03.001 · 5.98 Impact Factor
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    Biomaterials 11/2012; 33(33):8743–8744. DOI:10.1016/j.biomaterials.2012.08.002 · 8.31 Impact Factor
  • Chandana Mohanty, Manasi Das, Sanjeeb K Sahoo
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    ABSTRACT: Introduction: Curcumin is a safe, affordable and natural bioactive molecule of turmeric (Curcuma longa). It has gained considerable attention in recent years for its multiple pharmacological activities. However, its optimum pharmaceutical potential has been limited by its lack of aqueous solubility and poor bioavailability. To mitigate the above limitations, recently various nanostructured water-soluble delivery systems were developed to increase the solubility and bioavailability of curcumin. Areas covered: Major reasons contributing to the low bioavailability of curcumin appear to be owing to its poor solubility, low absorption, rapid metabolism and rapid systemic elimination. The present review summarizes the strategies using curcumin in various nanocarrier delivery systems to overcome poor solubility and inconsistent bioavailability of curcumin and describes the current status and challenges for the future. Expert opinion: The development of various drug delivery systems to deliver curcumin will certainly provide a step up towards augmenting the therapeutic activity of curcumin thereby increasing the solubility and bioavailability of curcumin. However, the future of such delivery technology will be highly dependent on the development of safe, non-toxic and non-immunogenic nanocarriers.
    Expert Opinion on Drug Delivery 09/2012; 9(11):1347-64. DOI:10.1517/17425247.2012.724676 · 4.12 Impact Factor

Publication Stats

5k Citations
355.03 Total Impact Points

Institutions

  • 2008–2012
    • All India Institute of Medical Sciences Bhubaneswar
      Bhubaneswar, Orissa, India
  • 2002–2008
    • University of Nebraska Medical Center
      • Department of Pharmaceutical Sciences
      Omaha, Nebraska, United States
  • 2004–2005
    • The Nebraska Medical Center
      Omaha, Nebraska, United States