-
[show abstract]
[hide abstract]
ABSTRACT: The aims of the present study are to quantitatively analyze survivin expression, its clinicopathologic roles, and correlation with telomerase activity in a large cohort of patients with colorectal adenocarcinoma. Real-time polymerase chain reaction was used to quantitate expression level of survivin messenger RNA and human telomerase reverse transcriptase messenger RNA (telomerase activity) in 51 patients with colorectal adenocarcinomas. The findings were correlated with the clinicopathologic features of patients, which were prospectively collected into a computerized database. Survivin messenger RNA was expressed in all tumor samples. The level of expression in tumor tissues was increased in comparison with matched nontumor mucosa in the same patient (P = .01). The level of expression of survivin was significantly correlated with the level of human telomerase reverse transcriptase expression (P = .008) and size of the colorectal adenocarcinomas (P = .004). Survival of the patients with colorectal adenocarcinoma was associated with the TNM stages (P = .001) and not with the level of expression of survivin. Thus, survivin activity was altered in colorectal adenocarcinoma. The high prevalence of survivin expression and correlation with telomerase activity are important factors for consideration in gene targeting therapy for colorectal adenocarcinoma.
Human pathology 07/2008; 39(8):1229-33. · 3.03 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Human telomerase reverse transcriptase (hTERT) is believed to a reliable marker for telomerase activity. The expression of telomerase activity has not been investigated in a consecutive series of patients with colorectal adenocarcinoma in North Queensland. The objective of this study was to evaluate the significance of hTERT mRNA expression in colorectal adenocarcinoma in North Queensland.
Matched samples of tumour and adjacent non-tumorous mucosa samples from 53 colorectal carcinomas and nine colorectal adenomas were collected. In all these samples, RNA was extracted and then transcribed to cDNA. Real-time polymerase chain reaction (RT-PCR) was used to quantitate expression the level of hTERT mRNA. The findings were correlated with the clinicopathological features of patients with these tumours prospectively collected into a computerised database.
hTERT mRNA was expressed in all tumour samples. The level of expression in the colorectal adenocarcinomas was significantly higher than the corresponding non-tumorous mucosa (p = 0.009, t-test). The level of expression in the adenocarcinomas was slightly higher than those of adenomas, but the difference was not statistically significant. A higher level of hTERT expression was often noted in the adenocarcinomas arising from the left colon and rectum when compared with those from the right colon (p = 0.029).
Colorectal adenocarcinoma revealed expression of telomerase hTERT mRNA, which was detected quantitatively by real-time PCR. hTERT could be a potential biomarker for colorectal cancer. The difference between proximal and distal colorectum in hTERT expression could account for their known different clinical behaviour.
Pathology 02/2008; 40(1):25-30. · 2.38 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Basal cell carcinoma (BCC) is the most common skin cancer in North Queensland, Australia. The role of telomerase in BCC has not been investigated in this region. The objective of the study was to investigate the expression levels of human telomerase reverse transcriptase (hTERT) and telomerase activity in nodular BCCs and superficial BCCs from patients living in North Queensland. Matched tumor and adjacent nontumorous mucosa samples from 12 BCCs (7 nodular and 5 superficial) among 11 male patients were collected. In these samples, RNA was extracted and then transcribed to complementary DNA. Real-time polymerase chain reaction (PCR) was used to quantify the expression levels of hTERT messenger RNA (mRNA). In addition, telomeric repeat amplification protocol assay, a PCR-based method, was performed to detect telomerase activity in tissue extracts from these samples. The findings were correlated with the clinicopathologic features of patients with these tumors. All BCC samples expressed telomerase hTERT mRNA, and 75% (9/12) of the BCC samples showed telomerase activity. The mean level of expression among BCC samples was higher than that among matched nontumor samples (mean, 0.468 versus 0.140; P < .0001). Nodular BCC samples showed higher expression levels of hTERT mRNA as compared with superficial BCC samples (mean, 0.584 versus 0.305; P < .05). There was no significant association between expression of hTERT mRNA and tumor size. In conclusion, this study is the first on telomerase expression in BCC in Queensland as well as the first to analyze telomerase in BCC by quantitative PCR. The results indicate that telomerase alterations have an important role in the pathogenesis of BCCs. A unique finding is that the telomerase expression level in nodular BCCs is different from that in superficial BCCs.
Human Pathlogy 07/2007; 38(7):1023-9. · 2.88 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: This report describes the presence of a unique dual domain carbonic anhydrase (CA) in the giant clam, Tridacna gigas. CA plays an important role in the movement of inorganic carbon (Ci) from the surrounding seawater to the symbiotic algae that are found within the clam's tissue. One of these isoforms is a glycoprotein which is significantly larger (70 kDa) than any previously reported from animals (generally between 28 and 52 kDa). This alpha-family CA contains two complete carbonic anhydrase domains within the one protein, accounting for its large size; dual domain CAs have previously only been reported from two algal species. The protein contains a leader sequence, an N-terminal CA domain and a C-terminal CA domain. The two CA domains have relatively little identity at the amino acid level (29%). The genomic sequence spans in excess of 17 kb and contains at least 12 introns and 13 exons. A number of these introns are in positions that are only found in the membrane attached/secreted CAs. This fact, along with phylogenetic analysis, suggests that this protein represents the second example of a membrane attached invertebrate CA and it contains a dual domain structure unique amongst all animal CAs characterized to date.
FEBS Journal 08/2005; 272(13):3297-305. · 3.79 Impact Factor
-
-
-
