Publications (4)3.51 Total impact
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Article: Hormonal induction and semen characteristics of tambaqui Colossoma macropomum
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ABSTRACT: c1 All correspondence to: P. C. F. Carneiro. Laboratório de Biotecnologia da Reprodução Animal, Embrapa Tabuleiros Costeiros, Av. Beira Mar 3250, CEP 49025–040, Aracaju, SE, Brazil. Tel: +55 79 4009 1350. Fax: +55 79 4009 1303. e-mail: paulo@cpatc.embrapa.brZygote 01/2012; 20(01):39 - 43. · 1.17 Impact Factor -
Article: Hormonal induction and semen characteristics of tambaqui Colossoma macropomum.
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ABSTRACT: In the hatchery-bred tambaqui Colossoma macropomum, spontaneous semen release does not occur, and hand-stripping produces reduced semen volume. The goal of this work is to evaluate the effects of hormonal induction with carp pituitary extract (CPE) on both qualitative (visual aspect, pH, motility, viability and morphological abnormalities) and quantitative (volume, concentration and number of spermatozoa per ejaculate) traits of tambaqui semen. Eleven males were treated with CPE (induced), and 11 were left untreated as a control (non-induced). All analysed parameters except motility and percentage of viable spermatozoa presented significant differences (p < 0.05) between the induced and non-induced treatments. CPE induction resulted in a 25-fold increase in semen volume and a 10-fold increase in the number of spermatozoa collected. However, both sperm concentration and the frequency of sperm with morphological abnormalities (commonly detached heads or bent tails) were significantly lower in CPE-induced fish. The hormonal induction of tambaqui males with CPE is efficient and positively influences some qualitative and quantitative properties of semen. Additionally, semen collection via gentle abdominal massage occurs more readily in CPE-induced fish.Zygote 01/2011; 20(1):39-43. · 1.17 Impact Factor -
Article: Development of the neotropical catfish Rhamdia quelen (Siluriformes, Heptapteridae) incubated in different temperature regimes.
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ABSTRACT: The developmental stages for the embryonic and larval periods of the silver catfish (Rhamdia quelen) kept at different temperatures (21, 24, 27 and 30 degrees C) are described. Fish were analysed under light and scanning electron microscopy. For embryonic development, we described 25 stages, which were grouped into seven periods named zygote, cleavage, blastula, gastrula, segmentation, pharyngula and hatching periods. For larval development, we defined three stages (early, mid, and late larvae). Additionally, the main ontogenetic events during the post-larvae and early juvenile periods were also described. This species presents a well developed lateral line and chemosensory systems that grow up during the larval period, maturing in the post-larvae. All tested temperatures are viable to R. quelen development, but a shorter incubation period was necessary to complete the development at lower temperatures. However, some malformations (heart edema) were verified at 30 degrees C.Zygote 10/2009; 18(2):131-44. · 1.17 Impact Factor -
Article: Gonadotrofina coriônica humana e hormônio liberador de gonadotrofina como indutores da reprodução do jundiá = Human chorionic gonadotropin and gonadotropin-releasing hormone as breeding inducers of jundiá
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ABSTRACT: Este trabalho foi realizado com o objetivo de avaliar parâmetros reprodutivos de machos e fêmeas de jundiá adultos após a aplicação do HCG (gonadotrofina coriônica humana) e do GnRHa (hormônio liberador do hormônio gonadotrópico) e compará-los àqueles obtidos quando usado o extrato hipofisário da carpa comum. Etapa 1 (Machos) - quatro grupos, com oito machos cada, receberam os seguintes tratamentos: M1: sem hormônio; M2: extrato hipofisário 0,5 mg kg-1; M3: HCG 200 UI kg-1; M4: GnRHa+inibidor dopaminergético 0,1 glóbulo(Ovopel®) kg-1. Etapa 2 (fêmeas) – 36 fêmeas foram divididas em cinco grupos: F1: extrato hipofisário em duas aplicações, 0,5 e 5,0 mg kg-1; F2: HCG em duas aplicações, 200 e 400 UI kg-1; F3: HCG 400 UI kg-1 em doseúnica; F4: HCG 1000 UI kg-1 em dose única; F5: GnRHa+inibidor dopaminergético 1 glóbulo(Ovopel®) kg-1. O uso do extrato hipofisário da carpa comum aumentou significativamente o volume de sêmen liberado e estimulou maior quantidade de fêmeas à liberar óvulos. O HCG e o GnRHa não apresentaram resultados positivos no tocante à reprodução induzida do jundiá, nas doses utilizadas neste estudo. In this study we analyzed reproductive parameters of males and females of jundiá induced by HCG (human chorionic gonadotropin) and GnRHa (gonadotropin-releasing hormone), and compared the results tothose using carp pituitary extract. Stage 1 (Males) - four groups with eight males each received one of the following treatments: M1: without hormone; M2: pituitary extract 0.5 mg kg-1; M3: HCG 200 UI kg-1; M4: GnRHa+dopamine receptor antagonist 0.1 pellet (Ovopel®) kg-1. Stage 2 (Females) – thirty-six females were separated in 5 groups: F1: pituitary extract in two applications, 0.5 and 5.0 mg kg-1; F2: HCG in two applications, 200 and 400 UI kg-1; F3: HCG 400 UI kg-1 in a single dose; F4: HCG 1000 UI kg-1 in a singledose; F5: GnRHa+dopamine receptor antagonist 1 pellet (Ovopel®) kg-1. The pituitary extract significantly increased the volume of semen and was more efficient in the final ovulation process, stimulating a higher number of females. HCG does not induce jundiá breeding with doses employed in this study and GnRHa is not suitible to induce jundiá breeders under the dose recommended by the manufacturer for other tropical species.Acta Scientiarum : Animal Sciences. 01/2008;
