Z.A. Intan Zarina

University of Science Malaysia, Nibong Tebal, Pulau Pinang, Malaysia

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Publications (4)1.6 Total impact

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    ABSTRACT: This study determines the validity and reliability of the Malay version of the World Health Organization Quality of Life (WHOQOL) assessment instrument in patients with human immunodeficiency virus (HIV) infection. A cross-sectional study on 157 patients with HIV seen at the Infectious Disease Unit, Hospital Raja Perempuan Zainab II, Kota Bharu, Kelantan was conducted. Factor analysis identified five major domains: physical needs, spirituality, social relationship, psychological, and environment. Significant correlation was found between each domain scores and the general health questions. The instrument was able to discriminate between asymptomatic and symptomatic HIV positive patients for all domain scores except for the spirituality domain. The internal consistency of the five domains ranged from 0.70 to 0.83. The intraclass correlation coefficient (ICC) ranged from 0.60 to 0.87 across all domains. In conclusion, the Malay version of WHOQOL-HIV BREF is a valid and reliable instrument in assessing quality of life in HIV positive patients.
    AIDS Care 10/2009; 21(10):1271-8. · 1.60 Impact Factor
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    ABSTRACT: Problem statement: DNA samples from fourteen modern human bloods (seven males and seven females) and two ancient skeletal samples excavated from Kalumpang Island and Dungun, Peninsular Malaysia were subjected for molecular genders determination using specific primers of human AMELX and AMELY . Approach: A standard multiple PCR mixture with forward primer and either a human X-specific reverse primer for AMELX or a human Y-specific reverse primer for AMELY amplifications were used to assess the presence of these genes in male and female samples. Results: PCR amplification of a modern male sample yielded 329 and 235 bp bands, whereas a modern female sample only 329 bp band. The Kalumpang Island sample produced two positive bands of AMELY and AMELX . After reamplification of the Dungun sample, only an AMELY band was visible. All amplified bands were cloned into TA plasmid and sequenced. BLAST analysis showed that the 329 bp band is AMELX , while the 235 bp product is AMELY . Conclusion/Recommendations: The skeletal remains of both Kalumpang Island and Dungun samples from west and east of Peninsular Malaysia respectively, are males.
    American Journal of Applied Sciences. 01/2009;
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    ABSTRACT: The aim of this study is to differentiate peripheral blood mononucleated cells that have been isolated from ICR mice`s ( Mus musculus )peripheral blood into mature osteoblast and osteoclast cells with addition of growth factors. The mononucleated cells were isolated by density centrifugation using Ficoll-Paque<sup>TM</sup> Plus. The culture mediums were then supplemented with growth factors; 50 ng mL<sup>-1</sup> RANKL and 25 ng mL<sup>-1</sup> M-CSF to differentiate into osteoclast cells. On the other hand, osteoblast assay, 50μg mL<sup>-1</sup> ascorbic acid and 10 mM β-glycerophosphate were added to support differentiation. For control, the same cells were used without supplementation of respective growth factors. Biochemical analysis for osteoblast, i.e., Alkaline Phosphatase (ALP) activity was determined on day 3-14. The results showed that the ALP activity in the differentiation medium is significantly increased (p<0.05) on day 10 and day 14 as compared to control, i.e., cells without growth factors. Tartrate Resistant Acid Phosphatase (TRAP) activity that represented as osteoclast biomarker also showed significant increased (p<0.05) from day 3 until day 10 in the present of RANKL and M-CSF. The viability of differentiated cells also showed that the cells were able to survive until 10 to 14 days in the presence of respective growth factors without significant increased in respective differentiation medium. RT-PCR analysis on isolated RNA from mononucleated cells after 14 and 10 days in their differentiation medium showed that the osteoblast and osteoclast were expressing ALP (~373 bp) and TRAP (~281 bp) gene, respectively. Mononucleated cells originated from peripheral blood have the potential to differentiate into osteoblast and osteoclast cells in the presence of specific growth factors. The respective cells are primitive enough to differentiate into two distinct types of mature cells hence can be categorized as multipotent stem cells.
    Journal of Biological Sciences. 01/2008;
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    Zarina ZA, Zahiruddin O
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    ABSTRACT: Cognitive impairment cases referred to psychiatric clinic are very few especially those who have mild disease. Formal evaluation of cognitive impairment is time consuming and it involves consultation with an expert in psychological training. One clinically useful test for practitioners is the Mini Mental State Examination (MMSE). Although MMSE has shown good reliability and validity, using this instrument in a language and culture other than English may create problems. However the test has never been validated in Bahasa Malaysia even though it is a widely used screening test during patient's clinical examination. This study was done to translate and validate MMSE into Bahasa Malaysia.
    Malaysian Journal of Psychiatry. 01/2007; 16(1):16-19.