Kees Heuvelman

Publications (6)14.75 Total impact

• Source

  Available from: Amjad Mughal

  Dataset: Molecular typing of Bordetella parapertussis isolates circulating in Pakistan

  Habib Bokhari, Fahad Said, Muhammad A Syed, Amjad Mughal, Yasmin F Kazi, Teemu Kallonen, Qiushui He, Audrey J King, Kees Heuvelman, Frits R Mooi
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  ABSTRACT: Although a whole-cell pertussis vaccine was introduced in Pakistan in 1980, little is known about the pertussis prevalence and circulating strains in Pakistan. The aim of this study was to analyze Bordetella parapertussis isolates circulating between 2005 and 2009 in Pakistan and to compare them with those found in other countries during different periods. A total of 59 (7.35%) B. parapertussis isolates from 802 subjects (median age, 3 years) from Pakistan, with pertussis-like symptoms were investigated. We carried out genotyping and DNA microarray analyses on these isolates and compared them with some international isolates of B. parapertussis. We found that the allele for pertactin (prn) found in strains studied from Pakistan was identical to the predominant type found in Europe. We showed that B. parapertussis isolates circulating in Pakistan are part of the same pulsed-field gel electrophoresis group to those circulating in Finland during the period of 1982–2007. Finally, microarray analysis confirmed that the isolates collected in Pakistan, were quite similar to international strains. Overall, these results confirm that B. parapertussis is extremely monomorphic. The high isolation rate of B. parapertussis (7.35%) compared to Bordetella pertussis (0.5%) may suggest that the whole-cell vaccine used in Pakistan is effective against B. pertussis (0.5% infections detected), but much less so against B. parapertussis.
• Article: Molecular typing of Bordetella parapertussis isolates circulating in Pakistan.

  Habib Bokhari, Fahad Said, Muhammad A Syed, Amjad Mughal, Yasmin F Kazi, Teemu Kallonen, Qiushui He, Audrey J King, Kees Heuvelman, Frits R Mooi
  [show abstract] [hide abstract]
  ABSTRACT: Although a whole-cell pertussis vaccine was introduced in Pakistan in 1980, little is known about the pertussis prevalence and circulating strains in Pakistan. The aim of this study was to analyze Bordetella parapertussis isolates circulating between 2005 and 2009 in Pakistan and to compare them with those found in other countries during different periods. A total of 59 (7.35%) B. parapertussis isolates from 802 subjects (median age, 3 years) from Pakistan, with pertussis-like symptoms were investigated. We carried out genotyping and DNA microarray analyses on these isolates and compared them with some international isolates of B. parapertussis. We found that the allele for pertactin (prn) found in strains studied from Pakistan was identical to the predominant type found in Europe. We showed that B. parapertussis isolates circulating in Pakistan are part of the same pulsed-field gel electrophoresis group to those circulating in Finland during the period of 1982-2007. Finally, microarray analysis confirmed that the isolates collected in Pakistan, were quite similar to international strains. Overall, these results confirm that B. parapertussis is extremely monomorphic. The high isolation rate of B. parapertussis (7.35%) compared to Bordetella pertussis (0.5%) may suggest that the whole-cell vaccine used in Pakistan is effective against B. pertussis (0.5% infections detected), but much less so against B. parapertussis.
  FEMS Immunology & Medical Microbiology 12/2011; 63(3):373-80. · 2.44 Impact Factor
• Article: Characterization of Bordetella holmesii isolates from patients with pertussis-like illness in The Netherlands.

  Frits R Mooi, Sylvia Bruisten, Ineke Linde, Frans Reubsaet, Kees Heuvelman, Saskia van der Lee, Audrey J King
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  ABSTRACT: Bordetella holmesii is a recently described human pathogen mainly isolated from blood. However, in the US and Canada, B. holmesii has also been cultured from the nasopharynx of patients with pertussis-like symptoms. To the best of our knowledge, respiratory isolates from Europe have not been characterized. Here, we report the isolation and characterization of B. holmesii from Dutch patients with pertussis-like illness. Species determination was confirmed by 16S rRNA gene sequencing and detection by PCR of IS481 and bhoE, a gene not found in Bordetella pertussis but present in B. holmesii. Comparative genomic hybridization (CGH) with microarrays revealed that the Dutch isolates formed a cluster distinct from isolates from the US and UK suggesting a distinct population or an epidemiological relationship between the Dutch isolates. All isolates contained a locus involved in iron uptake, previously suggested to originate from B. pertussis. The causes for the apparent increase in the isolation of B. holmesii are discussed.
  FEMS Immunology & Medical Microbiology 11/2011; 64(2):289-91. · 2.44 Impact Factor
• Article: Whooping cough in Pakistan: Bordetella pertussis vs Bordetella parapertussis in 2005-2009.

  Habib Bokhari, Fahad Said, Muhammad A Syed, Amjad Mughal, Yasmeen F Kazi, Kees Heuvelman, Frits R Mooi
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  ABSTRACT: Pertussis, or whooping cough, is an acute respiratory disease mainly affecting infants and children and is caused by Bordetella pertussis and Bordetella parapertussis. The aim of this study was to investigate the share of Bordetella species from potential whooping cough cases during 2005-2009. Eight hundred and two samples from suspected pertussis cases were collected, mainly from 2 provinces of Pakistan. Bacterial culture, identification, DNA extraction and routinely used polymerase chain reaction (PCR) methods using IS1001, IS1002 and IS481 were used to identify the Bordetella species. The results were unexpected, because all of the isolates collected from the different cities were identified as B. parapertussis (7.4%); B. pertussis was not isolated from any sample. However, PCR results indicated the presence of a small percentage (0.6%) of B. pertussis among the total cases studied. This study suggests that vaccines to protect against both B. pertussis and B. parapertussis should be considered.
  Scandinavian Journal of Infectious Diseases 05/2011; 43(10):818-20. · 1.72 Impact Factor
• Article: Correction: Comparative genomic profiling of Dutch clinical Bordetella pertussis isolates using DNA microarrays: identification of genes absent from epidemic strains.

  Audrey J King, Tamara van Gorkom, Jeroen LA Pennings, Han Gj van der Heide, Qiushui He, Dimitri Diavatopoulos, Kees Heuvelman, Marjolein van Gent, Karin van Leeuwen, Frits R Mooi
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  ABSTRACT: ABSTRACT: Correction After the publication of this work (King et al, 2008) we found out that Table 1 was not filled in correctly in the original article. The total number of strains analyzed in the ptxP1 or the ptxP3 lineage was not shown between the brackets, leading to incompleteness of the data. The revised Table 1 is shown below. We regret any inconvenience that the incompleteness of Table 1 in the original article might have caused.
  BMC Genomics 03/2010; 11(1):196. · 4.07 Impact Factor
• Source

  Available from: Qiushui He

  Article: Comparative genomic profiling of Dutch clinical Bordetella pertussis isolates using DNA microarrays: identification of genes absent from epidemic strains.

  Audrey J King, Tamara van Gorkom, Jeroen L A Pennings, Han G J van der Heide, Qiushui He, Dimitri Diavatopoulos, Kees Heuvelman, Marjolein van Gent, Karin van Leeuwen, Frits R Mooi
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  ABSTRACT: Whooping cough caused by Bordetella pertussis in humans, is re-emerging in many countries despite vaccination. Several studies have shown that significant shifts have occurred in the B. pertussis population resulting in antigenic divergence between vaccine strains and circulating strains and suggesting pathogen adaptation. In the Netherlands, the resurgence of pertussis is associated with the rise of B. pertussis strains with an altered promoter region for pertussis toxin (ptxP3). We used Multi-Locus Sequence Typing (MLST), Multiple-Locus Variable Number of Tandem Repeat Analysis (MLVA) and microarray-based comparative genomic hybridization (CGH) to characterize the ptxP3 strains associated with the Dutch epidemic. For CGH analysis, we developed an oligonucleotide (70-mers) microarray consisting of 3,581 oligonucleotides representing 94% of the gene repertoire of the B. pertussis strain Tohama I. Nine different MLST profiles and 38 different MLVA types were found in the period 1993 to 2004. Forty-three Dutch clinical isolates were analyzed with CGH, 98 genes were found to be absent in at least one of the B. pertussis strains tested, these genes were clustered in 8 distinct regions of difference. The presented MLST, MLVA and CGH-analysis identified distinctive characteristics of ptxP3 B. pertussis strains -the most prominent of which was a genomic deletion removing about 23,000 bp. We propose a model for the emergence of ptxP3 strains.
  BMC Genomics 07/2008; 9:311. · 4.07 Impact Factor