Manoel Sant'anna Filho

Pontifícia Universidade Católica do Rio Grande do Sul, Pôrto de São Francisco dos Casaes, Rio Grande do Sul, Brazil

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Publications (3)12.32 Total impact

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    ABSTRACT: Emerging evidence suggests that endothelial cell-secreted factors contribute to the pathobiology of squamous cell carcinoma (SCC) by enhancing invasive migration and resistance to anoikis. Here we report that SCC cells within the perivascular niche have undergone epithelial to mesenchymal transition (EMT) in a primary human SCC of a patient that developed distant metastases. Endothelial cell-secreted EGF induced EMT of human SCC cells in vitro and also induced acquisition of a stem-like phenotype. In vivo, tumor xenografts vascularized with EGF-silenced endothelial cells exhibited a smaller fraction of cancer stem-like cells (ALDH+CD44+) and were less invasive than tumors vascularized with control endothelial cells. Collectively, these results demonstrated that endothelial cell-EGF induces EMT and an acquisition of stem-like properties by head and neck tumor cells. On this basis, we suggest that vascular endothelial cells contribute to tumor dissemination by secreting factors that endow carcinoma cells with enhanced motility and stemness.
    Cancer Research 03/2014; 74(10). DOI:10.1158/0008-5472.CAN-13-2032 · 9.28 Impact Factor
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    ABSTRACT: This study evaluated the action of low-level laser therapy (LLLT) on the modulation of inflammatory reactions during wound healing in comparison with meloxicam. LLLT has been recommended for the postoperative period because of its ability to speed healing of wounds. However, data in the literature are in disagreement about its anti-inflammatory action. Standardized circular wounds were made on the backs of 64 Wistar rats. The animals were divided into four groups according to the selected postoperative therapy: group A-control; group B-administration of meloxicam; and groups C and D-irradiation with red (lambda = 685 nm) and infrared (lambda = 830 nm) laser energy, respectively. The animals were killed at 12, 36, and 72 h and 7 days after the procedure. Microscopic analysis revealed significant vascular activation of irradiated sites in the first 36 h. Only group B showed decreases in the intensity of polymorphonuclear infiltrates and edema. Group D showed a higher degree of organization and maturation of collagen fibers than the other groups at 72 h. The animals in group C showed the best healing pattern at 7 days. The anti-inflammatory action of meloxicam was confirmed by the results obtained in this research. The quantification of interleukin-1beta (IL-1beta) mRNA by real-time polymerase chain reaction (PCR) did not show any reduction in the inflammatory process in the irradiated groups when compared to the other groups. LLLT improves the quality of histologic repair and is useful during wound healing. However, with the methods used in this study the laser energy did not minimize tissue inflammatory reactions.
    Photomedicine and Laser Surgery 01/2008; 25(6):467-73. DOI:10.1089/pho.2007.1098 · 1.58 Impact Factor
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    ABSTRACT: Investigate the distribution of epidermal growth factor receptor (EGFR) in pericoronal follicles as a predictor of progression to odontogenic cysts and tumors. Immunohistochemical EGFR staining patterns (membrane-only, cytoplasm-only, or combined membrane and cytoplasmic staining) in the reduced enamel epithelium and nests of odontogenic epithelium associated with follicles of impacted molar teeth were evaluated. The staining pattern of 20 specimens of pericoronal follicle was compared with that of 16 normal oral mucosa samples and to squamous cell carcinoma samples. Combined membrane and cytoplasmic staining was observed for normal oral mucosa mostly in proliferating layers (basal and suprabasal), decreasing in intensity toward the surface. Seven epithelial nests presented membrane-only staining, and the majority presented either a cytoplasm-only or a combined staining pattern. The staining patterns observed in reduced enamel epithelium were cytoplasm-only and combined. EGFR membrane-only staining may be an indicator of increased potential for epithelial nests to become odontogenic cysts or tumors.
    Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 03/2007; 103(2):240-5. DOI:10.1016/j.tripleo.2005.11.009 · 1.46 Impact Factor