Dong Chen

Publications (3)5.99 Total impact

• Source

  Available from: im.ac.cn

  Article: [Preparation of functional chitosan thermosensitive hydrogel for slow release both rhBMP-2 and chlorhexidine].

  Zhi-Wei Ma, Rong Wang, Zhi-Fen Wu, Dong Chen, Bang-Le Zhang, Wei He, Xiao-Juan Wang, Qing Liu, Jie Xu, Hao Zhu
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  ABSTRACT: The chitosan thermosensitive hydrogel is liquid at room temperature but gels rapidly when heated to body temperature. This hydrogel are wildly used for cell encapsulation, drug delivery or tissue-engineered scaffolds. The system can sustain the release of macromolecules over a period of several hours to a few days. However, with low-molecular-weight compounds, the release is generally completed within 24 h. To prepare a functional chitosan thermosensitive hydrogel for slow release both broad-spectrum antibiotic chlorhexidine and growth factor recombined human bone morphogenetic protein-2 (rhBMP-2), The beta-cyclodextrin was used to prepare an inclusion complex with chlorhexidine, and then the latter was incorporated into the chitosan thermosensitive hydrogel system. Simultaneously, rhBMP-2 was added into the hydrogel system. By HAAKE viscosity measuring instrument, we contrasted the viscoelastic properties of system with or without objective factors. And the in vitro release kinetics of chlorhexidine and rhBMP-2 was investigated by HPLC (high performance liquid chromatography) and ELISA (enzyme-linked immunosorbent assay) respectively. The results showed that the addition of chlorhexidine/beta-cyclodextrin inclusion complex to the thermosensitive solution did not change the gelling behavior of the thermosensitive system. Further, the in vitro release profiles demonstrated that the release rate of chlorhexidine and rhBMP-2 from hydrogel became slower, controlled delivery over at least 1 month. By first preparing chlorhexidine/beta-cyclodextrin inclusion complex, and then mixing the IC and rhBMP-2 into the chitosan thermosensitive hydrogel, a functional chitosan thermosensitive hydrogel system with ability of slow release both rhBMP-2 and chlorhexidine is successfully made.
  Sheng wu gong cheng xue bao = Chinese journal of biotechnology 12/2007; 23(6):1049-54.
• Article: MMP-2, MMP-9 and TIMP-2 gene polymorphisms in Chinese patients with generalized aggressive periodontitis.

  Dong Chen, Qi Wang, Zhi-Wei Ma, Fa-Ming Chen, Yue Chen, Guang-Yuan Xie, Qin-Tao Wang, Zhi-Fen Wu
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  ABSTRACT: Aggressive periodontitis (AgP) has a genetic basis. It has been reported that the functional gene polymorphisms of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) alter their expressions in transcriptional level and they are involved in the tissue destruction of periodontitis. The study was carried out to analyse the association of functional polymorphisms in MMP-2, MMP-9 and TIMP-2 with generalized AgP (G-AgP) in a Chinese population. The study population consisted of 79 Chinese patients with G-AgP and 128 healthy controls. DNA was obtained from oral mucosa swab samples. MMP-2 genotypes were determined by PCR-based denaturing high-performance liquid chromatography analysis while MMP-9 and TIMP-2 genotypes were identified by a PCR-based restriction fragment length polymorphism. Chi2 test after Yates' correction was used to investigate the possible association of the genotypes with the G-AgP. Although gene polymorphisms for MMP-2 and MMP-9 did not show any association with the G-AgP, the analysis of the TIMP-2 -418G to C gene polymorphism revealed significant differences between the patients and controls. Compared with controls, a significant increasing trend of TIMP-2 -418C carrier in the G-AgP patients occurred (p=0.013). It is suggested that the TIMP2 -418G to C gene polymorphism is associated with G-AgP in the Chinese subjects.
  Journal Of Clinical Periodontology 06/2007; 34(5):384-9. · 3.00 Impact Factor
• Article: MMP‐2, MMP‐9 and TIMP‐2 gene polymorphisms in Chinese patients with generalized aggressive periodontitis

  Dong Chen, Qi Wang, Zhi-Wei Ma, Fa-Ming Chen, Yue Chen, Guang-Yuan Xie, Qin-Tao Wang, Zhi-Fen Wu
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  ABSTRACT: Background: Aggressive periodontitis (AgP) has a genetic basis. It has been reported that the functional gene polymorphisms of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) alter their expressions in transcriptional level and they are involved in the tissue destruction of periodontitis. The study was carried out to analyse the association of functional polymorphisms in MMP-2, MMP-9 and TIMP-2 with generalized AgP (G-AgP) in a Chinese population.Material and Methods: The study population consisted of 79 Chinese patients with G-AgP and 128 healthy controls. DNA was obtained from oral mucosa swab samples. MMP-2 genotypes were determined by PCR-based denaturing high-performance liquid chromatography analysis while MMP-9 and TIMP-2 genotypes were identified by a PCR-based restriction fragment length polymorphism. χ2 test after Yates' correction was used to investigate the possible association of the genotypes with the G-AgP.Results: Although gene polymorphisms for MMP-2 and MMP-9 did not show any association with the G-AgP, the analysis of the TIMP-2 −418G to C gene polymorphism revealed significant differences between the patients and controls. Compared with controls, a significant increasing trend of TIMP-2 −418C carrier in the G-AgP patients occurred (p=0.013).Conclusion: It is suggested that the TIMP2 −418G to C gene polymorphism is associated with G-AgP in the Chinese subjects.
  Journal Of Clinical Periodontology 04/2007; 34(5):384 - 389. · 3.00 Impact Factor