Megumi Tsuchiya

Publications (2)2.79 Total impact

• Source

  Available from: PubMed Central

  Article: Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification.

  Tatsuo Ito, Neelu Yadav, Jaeho Lee, Takayuki Furumatsu, Satoshi Yamashita, Kenji Yoshida, Noboru Taniguchi, Megumi Hashimoto, Megumi Tsuchiya, Toshifumi Ozaki, Martin Lotz, Mark T Bedford, Hiroshi Asahara
  [show abstract] [hide abstract]
  ABSTRACT: Chondrogenesis and subsequent endochondral ossification are processes tightly regulated by the transcription factor Sox9 (SRY-related high mobility group-Box gene 9), but molecular mechanisms underlying this activity remain unclear. Here we report that coactivator-associated arginine methyltransferase 1 (CARM1) regulates chondrocyte proliferation via arginine methylation of Sox9. CARM1-null mice display delayed endochondral ossification and decreased chondrocyte proliferation. Conversely, cartilage development of CARM1 transgenic mice was accelerated. CARM1 specifically methylates Sox9 at its HMG domain in vivo and in vitro. Arg-methylation of Sox9 by CARM1 disrupts interaction of Sox9 with beta-catenin, regulating Cyclin D1 expression and cell cycle progression of chondrocytes. These results establish a role for CARM1 as an important regulator of chondrocyte proliferation during embryogenesis.
  BMC Developmental Biology 10/2009; 9:47. · 2.79 Impact Factor
• Article: [Gene networks in chondrogenesis].

  Megumi Tsuchiya, Hiroshi Asahara
  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 11/2007; 52(12):1432-7.