Hiroyuki Araki

Publications (2)1.79 Total impact

• Source

  Available from: InTech

  Chapter: Improvement of Atopic Dermatitis by Human Sebaceous Fatty Acids and Related Lipids

  Hiroyuki Araki, Yoshiya Sugai, Hirofumi Takigawa
  02/2012; , ISBN: 978-953-51-0110-9
• Source

  Available from: Susumu Ito

  Article: Hyperproduction of sebaceous cis-6-hexadecenoic acid by esterase-reduced mutant of Rhodococcus sp. strain.

  Hiroyuki Araki, Hiroshi Hagihara, Hirofumi Takigawa, Nobuharu Kotani, Yukiharu Tsujino, Kenzo Koike, Shuji Kawai, Katsuya Ozaki, Susumu Ito
  [show abstract] [hide abstract]
  ABSTRACT: cis-6-Hexadecenoic acid is a major component of human sebaceous lipids that is involved in skin self-sterilization and atopic dermatitis amelioration. It can be prepared by hydrolysis of isopropyl cis-6-hexadecenoate produced by resting cells of Rhodococcus sp. strain KSM-MT66. To devise an economical industrial-scale process for the production of this rare fatty acid, we optimized the conditions for growing rhodococcal cells. Mg(2+) and Fe(2+) ions are essential for the efficient production of isopropyl cis-6-hexadecenoate. To further increase the production of isopropyl cis-6-hexadecenoate, we created a mutant strain (T64) with reduced esterase activity by random mutagenesis using UV irradiation of MT66. Under an optimized condition, the mutant T64 produced more than 60 g l(-1) isopropyl cis-6-hexadecenoate in a 4-d cultivation, corresponding to about 52 g l(-1)cis-6-hexadecenoate.
  Journal of Bioscience and Bioengineering 11/2007; 104(4):300-3. · 1.79 Impact Factor