Yuqing Zhao

Shenyang Pharmaceutical University, Feng-t’ien, Liaoning, China

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Publications (39)89.97 Total impact

  • Chen Zhao · Guangyue Su · Xude Wang · Xiaoshu Zhang · Shuang Guo · Yuqing Zhao ·
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    ABSTRACT: Objectives: 25-Hydroxyprotopanaxadiol (25-OH-PPD) and 25-methoxylprotopanaxadiol (25-OCH3-PPD), two ginseng sapogenins, have potent antitumor activity and their effects on gastric cancer (BGC-823, SGC-7901, MKN-28) cells and a gastric mucosa (GES-1) cell line are reported. Results: Both compounds significantly inhibited the growth of gastric cancer cells, while having lesser inhibitory effects on GES-1 cells by MTT assay. A mechanistic study revealed that the two ginseng sapogenins could induce apoptosis in BGC-823 cells by morphological observation, DNA fragmentation, flow cytometry and western blot analysis. Besides, the apoptosis was inhibited by Ac-DEVD-CHO, a caspase 3 inhibitor, which was confirmed by cell viability analysis. Conclusions: These results indicate that 25-OH-PPD and 25-OCH3-PPD have potential to be promising agents for the treatment of gastric cancer.
    Biotechnology Letters 10/2015; DOI:10.1007/s10529-015-1964-4 · 1.59 Impact Factor
  • Sainan Zhang · Yun Tang · Jiaqing Cao · Chen Zhao · Yuqing Zhao ·
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    ABSTRACT: 25-OCH3-PPD is a promising antitumor dammarane sapogenin isolated from the total saponin-hydrolyzed extract of Panax ginseng berry and Panax notoginseng leaves. 20(R)-25-OCH3-PPD was more potent as an anti-cancer agent than 20(S)-25-OCH3-PPD and epimeric mixture of 25-OCH3-PPD. This paper describes the rapid separation process of the R-epimer of 25-OCH3-PPD from its epimeric mixture by crystallization-induced dynamic resolution (CIDR). The optimized CIDR process was based on single factor analysis and nine well-planned orthogonal design experiments (OA9 matrix). A rapid and sensitive reverse phase high-performance liquid chromatographic (HPLC) method with evaporative light-scattering detector (ELSD) was developed and validated for the quantitation of 25-OCH3-PPD epimeric mixture and crystalline product. Separation and quantitation were achieved with a silica column using a mobile phase consisting of methanol and water (87:13, v/v) at a flow rate of 1.0mL/min. The ELSD detection was performed at 50°C and 3L/min. Under conditions involving 3mL of 95% ethanol, 8% HCl, and a hermetically sealed environment for 72h, the maximum production of 25(R)-OCH3-PPD was achieved with a chemical purity of 97% and a total yield of 87% through the CIDR process. The 25(R)-OCH3-PPD was nearly completely separated from the 220mg 25-OCH3-PPD epimeric mixture. Overall, a simple and steady small-batch purification process for the large-scale production of 25(R)-OCH3-PPD from 25-OCH3-PPD epimeric mixture was developed.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 09/2015; 1005. DOI:10.1016/j.jchromb.2015.09.011 · 2.73 Impact Factor
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    ABSTRACT: Eighteen compounds (1-18), seven new (3-9) and eleven previously reported (1, 2, and 10-18), were isolated from the flowers of Impatiens balsamina (Linn). The structures of the isolated compounds were elucidated using different spectroscopic methods, including NMR (1D and 2D), UV, IR, and HR-ESI-MS. Analysis of the bioassay results showed the compounds had notable anti-hepatic fibrosis activity against murine Hepatic Stellate Cells (t-HSC/Cl-6) and anti-diabetics activity against α-glucosidase. Specifically, new compounds 7, 8, 9 showed significant inhibitory activity on t-HSC/Cl-6 cells with IC50 values of 42.12, 109.2, 34.04μg/mL respectively, while the IC50 values of positive control Silymarin and Fufang Biejia Ruangan Pian were 202.34 and 231.56μg/mL, respectively. In addition, compounds 2, 4, 7, 8, 10, 11, 17, and 18 exhibited excellent α-glucosidase inhibitory activity. Amongst these compounds, 7 exhibited the highest activity with an IC50 value of 0.72μg/mL, while the IC50 value of the positive control acarbose was 3.36μg/mL. This is the first study evaluating the anti-hepatic fibrosis and anti-diabetic activities of compounds isolated from the flowers of I. Balsamina. Copyright © 2015. Published by Elsevier B.V.
    Fitoterapia 07/2015; 105. DOI:10.1016/j.fitote.2015.07.007 · 2.35 Impact Factor
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    ABSTRACT: Green asparagus (Asparagus officinalis L.) is a vegetable with numerous nutritional properties. In the current study, a total of 23 compounds were isolated from green asparagus, and 9 of these compounds were obtained from this genus for the first time. Preliminary data showed that the ethyl acetate (EtOAc)-extracted fraction of green asparagus exerted a stronger inhibitory effect on the growth of t-HSC/Cl-6 cells, giving an IC50 value of 45.52 μg/mL. The biological activities of the different compounds isolated from the EtOAc-extracted fraction with respect to antihepatic fibrosis were investigated further. Four compounds, C3, C4, C10, and C12, exhibited profound inhibitory effect on the activation of t-HSC/Cl-6 cells induced by TNF-α. The activation t-HSC/Cl-6 cells, which led to the production of fibrotic matrix (TGF-β1, activin C) and accumulation of TNF-α, was dramatically decreased by these compounds. The mechanisms by which these compounds inhibited the activation of hepatic stellate cells appeared to be associated with the inactivation of TGF-β1/Smad signaling and c-Jun N-terminal kinases, as well as the ERK phosphorylation cascade.
    Journal of Agricultural and Food Chemistry 06/2015; 63(26). DOI:10.1021/acs.jafc.5b01490 · 2.91 Impact Factor
  • Jiaqing Cao · Wei Li · Yun Tang · Ning Li · Yuqing Zhao ·
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    ABSTRACT: A new cyclic bisdesmoside treterpene saponin, lobatoside N (1), together with four known triterpenoids, was isolated from the herb of Actinostemma lobatum Maxim. Structures were established by means of extensive spectral data analysis. Furthermore, the cytotoxic activities of the identified compounds were evaluated using HCT-116, HT-29, MCF-7 and A549 human cancer cell lines. As a result, compounds 1-5 showed significant cytotoxicities in a dose-dependent manner against the cell lines tested. Especially, compound 5 exhibited stronger activities, with IC50 value of 0.88 μM and 0.98 μM, than that of the positive control drug (Cis-platinum) against HT-29 and A549 cell lines.
    Pharmazie 05/2015; 70(5). DOI:10.1691/ph.2015.4881 · 1.05 Impact Factor
  • Weizhuo Tang · Jiaqing Cao · Xiaoshu Zhang · Yuqing Zhao ·
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    ABSTRACT: Osmanthus fragrans (OF) is widely used as a flavour additive for tea and other beverages in China and some Asian countries. In this paper, phytochemical investigation on OF seed, the purple-brown ripe fruit of OF, resulted in the discovery of a new secoiridoid glucoside, fragranside A (1), together with nine known secoiridoid glucosides (2–10). Their structures were established through 1D and 2D NMR, HRESI-MS spectra, and compared with literature data. By HPLC analysis, nuzhennoside (4) and nuzhennoside G13 (6) were found to be the predominant compounds in OF seeds with a content of 0.87 and 1.23%, respectively. For quality control, they were simultaneously quantified from four representative species of OF seeds. Additionally, antioxidant and antiplatelet aggregation activity of OF seed extracts were first examined. Results showed that OF seed extracts and compounds 4 and 6 had obvious inhibiting effects on platelet aggregation induced by using adenosine 5′-diphosphate (ADP), collagen, and arachidonic acid (AA) as agonists. These findings suggest that OF seeds may have the potential to be developed into a novel inhibitor of platelet aggregation.
    Journal of Functional Foods 04/2015; 14. DOI:10.1016/j.jff.2015.02.001 · 3.57 Impact Factor
  • Zhenghong Guo · Xiuli Niu · Ting Xiao · Jingjing Lu · Wei Li · Yuqing Zhao ·
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    ABSTRACT: Licorice, one of the oldest and most common herbal medicines, is used as a natural sweetener and flavoring agent in food products. This study aimed to investigate the inhibitory activity of flavonoids against α-glycosidase and protein tyrosine phosphatase 1B (PTP1B). Macroporous resins were used to isolate total flavonoids from licorice extract; the obtained total flavonoid content was >80%. The extracted flavonoids were then identified by HPLC-MS/MS, and 18 compounds were isolated. These compounds were further evaluated to determine α-glycosidase and PTP1B inhibitory activities. A total of 12 compounds demonstrated stronger activity than acarbose (IC50 = 5.42 ± 0.10 µg/mL); the compound 18 yielded higher IC50 (15.62 ± 0.20 µM) than NaVO4 (positive control; IC50 = 29.10 ± 0.20 µM). These results suggested that flavonoids from licorice are potential functional food ingredients that can be used to prevent and treat type 2 diabetes mellitus.
    Journal of Functional Foods 04/2015; 14. DOI:10.1016/j.jff.2014.12.003 · 3.57 Impact Factor
  • Jiaqing Cao · Xiaoshu Zhang · Fanzhi Qu · Zhenghong Guo · Yuqing Zhao ·
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    ABSTRACT: Introduction: Dammarane triterpenoids, the main secondary metabolites of Panax ginseng, are very important natural compounds with remarkable biological activity. They could be isolated from the plants of Panax or other genus, as well as through the modifications of certain natural products. This review is a collection of a number of patents (2005 - 2014) that describe the dammarane triterpenoids for therapeutic or preventive uses on numerous common diseases. Areas covered: In this review, patents from 2005 to 2014 on chemical structures and treatment of different diseases by dammarane triterpenoids have been summarized. The SciFinder and the World Intellectual Property Organisation databases have been used as main sources for the search. Expert opinion: In the last decade, over 90 patents concerning dammarane derivatives for pharmaceutical have been published. These types of compounds could be used as agents for prevention and treatment of various kinds of diseases, such as cancer, diabetes mellitus and metabolic syndrome, hyperlipidemia, cardiovascular and cerebrovascular disease, aging, neurodegenerative disease, bone disease, liver disease, kidney disease, gastrointestinal disease, depression-type mental illness and skin aging. Rare plants, except for Panax genus, which contain dammarane triterpenoids should be studied extensively. In addition, more dammarane triterpenoids with good biological activity, especially the aglycones possessing novel side chain, should be prepared using chemical modification. Finally, pharmacological effects of dammarane triterpenoids should be further studied.
    Expert Opinion on Therapeutic Patents 04/2015; 25(7):1-13. DOI:10.1517/13543776.2015.1038239 · 4.30 Impact Factor
  • Yun Tang · Wei Li · Jiaqing Cao · Yuqing Zhao ·
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    ABSTRACT: Ethnopharmacological relevance: Bolbostemma paniculatum (Maxim.) Franquet (B. paniculatum), also named "Tu-bei-mu" in Chinese folk medicines, has been described in application for the treatment of tumors, warts, inflammation and toxication in traditional Chinese medicinal books. The major constituents in B. paniculatum are triterpenoid saponins, which have been proved to possess dramatically cytotoxic activity and antivirus activity. The aim of this study is to isolate and identify the active triterpenoid saponin from the bulb of B. paniculatum by a bioassay-guided method. Materials and methods: Four cucurbitacine triterpenoid sapogenins and 11 triterpenoid saponins were isolated from the active EtOAc and n-BuOH extract of B. paniculatum by using bioassay-guided screening. Their structures were elucidated based on the spectroscopic methods and compared with published data. Cytotoxic activities of isolated compounds were determined by MTT assay. Results: Four cucurbitacine triterpenoid sapogenins, isocucurbitacin B(1), 23,24-dihydroisocucurbitacin B(2), cucurbitacin E(3), 23,24-dihydrocucurbitacin E(4), and 11 triterpenoid saponins, tubeimosideI(5), tubeimoside III(6), tubeimoside V(7), dexylosyltubeimoside III(8), lobatoside C(9), tubeimoside A(10), tumeimoside B(11), lobatoside A(12), tubeimoside C(13), tubeimoside IV(14), 7β,18,20,26-tetrahydroxy-(20S)-dammar-24E-en-3-O-α-L-(4-acetyl)arabinopyranosyl-(1→2)-β-D-glucopyranoside(15) were isolated from the active EtOAc and n-BuOH extracts. Of them, compounds 2, 4, 9 and 12 were firstly isolated from the Bolbostemma genus. MTT assay revealed that compounds 1, 3 and 4 had significantly activities against HeLa and HT-29 human cancer cells with IC50 values ranging from 0.93 to 9.73μM. It is worth mentioning that compound 4׳s activities against the two cell lines are 12- and 8-fold that of the positive control drug (5-Fu). Whereas, the cyclic bisdesmosides 5-9 exerted significantly activities on BGC-823, HeLa, HT-29 and MCF-7 cancer cells with IC50 values ranging from 1.30 to 15.64μM. And 6׳s activities against the four cell lines are 6-, 3-, 10- and 16-fold that of 5-Fu and 8׳s activities against the four cell lines are 5-, 3-, 14- and 9-fold that of 5-Fu. Conclusion: The cytotoxic activity of the bulbs of B. paniculatum is mainly ascribable to cucurbitacine triterpenoid sapogenins (1-4) and the cyclic bisdesmosides (5-9). The cyclic bisdesmosides are the main anti-cancer active compounds of B. paniculatum. The above results provide scientific evidence to support, to some extent, the ethnomedicinal use of B. paniculatum as anticancer remedies in traditional Chinese medicine.
    Journal of ethnopharmacology 04/2015; 169. DOI:10.1016/j.jep.2015.04.003 · 3.00 Impact Factor
  • fanzhi qu · chen zhao · yafei liu · jiaqing cao · wei li · yuqing zhao ·

    Medicinal Chemistry Communication 01/2015; DOI:10.1039/C5MD00382B · 2.50 Impact Factor
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    Weizhuo Tang · Yuqing Zhao ·

    European Journal of Integrative Medicine 12/2014; 6(6). DOI:10.1016/j.eujim.2014.09.084 · 0.78 Impact Factor
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    Ting Xiao · Jiongmo Cui · Zhenghong Guo · Yuqing Zhao ·

    European Journal of Integrative Medicine 12/2014; 6(6). DOI:10.1016/j.eujim.2014.09.083 · 0.78 Impact Factor
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    Xiaoshu Zhang · Guohui Shi · Zhengting Liu · Jingxin Liu · Yuqing Zhao ·

    European Journal of Integrative Medicine 12/2014; 6(6). DOI:10.1016/j.eujim.2014.09.086 · 0.78 Impact Factor
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    Zhenghong Guo · Ting Xiao · Qian Li · Yuqing Zhao ·

    European Journal of Integrative Medicine 12/2014; 6(6). DOI:10.1016/j.eujim.2014.09.082 · 0.78 Impact Factor
  • Xiuli Bi · Wei Liu · Xichun Xia · Lili Chen · Teng Mou · Jingxin Liu · Yue Hou · yuqing zhao ·
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    ABSTRACT: The aim of this study was to investigate the effects of Callistephus chinensis flower (CCF) polyphones on symptoms of metabolic syndrome in a newly developed high-fat diet-induced Non-alcoholic fatty liver disease (NAFLD) mice model. C57BL/6J mice were fed a high fat diet (HFD; 50% energy as fat) with normal drinking fluid or HFD with CCF polyphones (50 mg/L or 100 mg/L) in drinking fluid for 12 wk. As a comparison, mice fed a normal-fat (NFD; 10% energy as fat) and with normal drinking fluid was also included. HFD group developed more body weight gain and severe symptoms of metabolic syndrome than the NFD group. CCF polyphones treatment significantly reduced body weight gain associated with increased fecal lipids compared to the NFD group. Liver damage and liver triglyceride levels were also decreased by CCF polyphones treatment. Moreover, both morphologic and histological detections indicated that CCF polyphones significantly reversed HFD-induced hepatic steatosis and liver injury. Furthermore, CCF polyphones significantly ameliorated HFD-induced metabolic disorders, including insulin resistance, inflammatory cytokines include interlukin-6 (IL-6), tumor necrosis factor-α (TNF-α). Besides, hepatic peroxisome proliferator-activated receptor (PPARα) and the gene involved in PPARα, Peroxisomal acyl-CoA oxidase (ACOX), were markedly up-regulated at protein levels by CCF polyphones. Our results demonstrate that the HFD produces metabolic syndrome of NAFLD and CCF polyphones treatment can alleviate these symptoms. The beneficial effects of CCF polyphones are associated with decreased lipid absorption and reduced levels of inflammatory cytokines.
    10/2014; 5(12). DOI:10.1039/C4FO00637B
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    ABSTRACT: Numerous compounds isolated from Ginseng have been shown to exhibit various biological activities, including antioxidant, anti-carcinogenic, anti-mutagenic, and anti-tumor activities. Recent research has focused on the potential values of these compounds in the prevention and treatment of human cancers. The anti-tumor activity of 25-hydroxyprotopanaxadiol (25-OH-PPD), a natural compound isolated from Panax ginseng, has been established in previous study. In the current study, we investigated the anti-tumor activity of three derivatives of 25-OH-PPD, namely xl, 1c, and 8b with respect to lung cancer. All three compounds significantly inhibited the growth of the human lung cancer cells A549 and H460. Oral administration of these compounds significantly inhibited the growth of xenograft tumors in mice without affecting body weight. Further mechanistic study demonstrated that these compounds could decrease the expression levels of β-catenin and its downstream targets Cyclin D1, CDK4, and c-myc in lung cancer cells. Taken together, the results suggested that the anti-growth activity exerted by these 25-OH-PPD derivatives against lung cancer cells probably involved β-catenin-mediated signaling pathway, a finding that could have important implication for chemotherapeutic strategy aiming at the treatment of lung cancer.
    European Journal of Pharmacology 09/2014; 742. DOI:10.1016/j.ejphar.2014.08.032 · 2.53 Impact Factor
  • Linlin Fang · Jiaqing Cao · Lili Duan · Yun Tang · Yuqing Zhao ·
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    ABSTRACT: Schisandra chinensis (Turcz.) Baill. (SCTB) is a functional, economical and medicinal herb that has numerous medicinal and nutritional properties. The present study was carried out to investigate its inhibitory activities against protein tyrosine phosphatase 1B (PTP1B) and α-glucosidase. The extracts and compounds isolated from petroleum ether (PE) extract were further tested for their PTP1B and α-glucosidase inhibitory activities. The data showed that the anti-diabetic activity of SCTB is attributable to the PE (IC50 = 1.77 ± 0.20 μg/mL) and EtOAc (IC50 = 1.04 ± 0.20 μg/mL) extracts as well as compounds 1 (IC50 = 2.36 ± 0.20 μM) and 4 (IC50 = 9.78 ± 0.10 μM) that were compared with the positive controls NaVO4 (IC50 = 22 ± 0.20 μM) and acarbose (IC50 = 2.10 ± 0.10 μg/mL), respectively. These results demonstrate that the stems of SCTB are useful as potential functional food ingredients for the prevention and treatment of type-2 diabetes.
    Journal of Functional Foods 07/2014; 9(1):264–270. DOI:10.1016/j.jff.2014.04.017 · 3.57 Impact Factor
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    ABSTRACT: The present study characterized in vitro metabolites of 20(R)-25-methoxyl-dammarane-3β, 12β, 20-triol (20(R)-25-OCH3-PPD) in mouse, rat, dog, monkey and human liver microsomes. 20(R)-25-OCH3-PPD was incubated with liver microsomes in the presence of NADPH. The reaction mixtures and the metabolites were identified on the basis of their mass profiles using LC-Q/TOF and were quantified using triple quadrupole instrument by multiple reaction monitoring. A total of 7 metabolites (M1-M7) of the phase I metabolites were detected in all species. 25(R)-OCH3-PPD was metabolized by hydroxylation, dehydrogenation, and O-demethylation. Enzyme kinetic of 20(R)-25-OCH3-PPD metabolism was evaluated in rat and human hepatic microsomes. Incubations studies with selective chemical inhibitors demonstrated that the metabolism of 20(R)-25-OCH3-PPD was primarily mediated by CYP3A4. We conclude that 20(R)-25-OCH3-PPD was metabolized extensively in mammalian species of mouse, rat, dog, monkey, and human. CYP3A4-catalyzed oxygenation metabolism played an important role in the disposition of 25(R)-OCH3-PPD, especially at the C-20 hydroxyl group.
    PLoS ONE 04/2014; 9(4):e94962. DOI:10.1371/journal.pone.0094962 · 3.23 Impact Factor
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    ABSTRACT: Three new compounds, a bicoumarin A (1), a biflavonoid A (2), and a biquinone A (3), as well as 12 other known compounds, were isolated from the flower of Lawsonia inermis L. The structures were elucidated by spectral analysis and new compounds 2 and 3 then were further confirmed by ECD calculations and single-crystal X-ray diffraction crystallography respectively. The cytotoxicity of the compounds against four cancer cell lines, including MCF-7, Hela, HCT-116, and HT-29 were evaluated using MTT assay. The IC50 values of compounds 3 and 5 against MCF-7, Hela, HCT-116, and HT-29 were 2.24, 1.42, 24.29, and 7.02 μM and 6.1, 2.44, 5.58, and 10.21 μM respectively. The two compounds exhibited stronger inhibitory activities than the positive control 5-fluorouracil (IC50 = 7.34, 11.50, 36.17, 18.83 μM) against the four tested cell lines. These results demonstrated that compounds from the flowers of L. inermis L. showed cytotoxic activity on MCF-7, Hela, HCT-116, and HT-29 cell lines.
    Fitoterapia 04/2014; 94. DOI:10.1016/j.fitote.2014.02.007 · 2.35 Impact Factor
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    ABSTRACT: The objective of this study was to develop a self-microemulsifying drug delivery system (SMEDDS) to enhance the oral bioavailability of the poorly water-soluble compound 20(S)-25-methoxydammarane-3β;12β;20-triol (25-OCH3-PPD). Optimized SMEDDS formulations for 25-OCH3-PPD contained Cremophor® EL (50%) as the surfactant, glycerin (20%) as the cosurfactant, and Labrafil® M1944 (30%) as the oil. The SMEDDS were characterized by morphological observation and mean droplet size. The pharmacokinetics and bioavailability of the 25-OCH3-PPD suspension and SMEDDS were evaluated and compared in rats. The plasma concentrations of 25-OCH3-PPD and its main metabolite, 25-OH-PPD, were determined by ultra performance liquid chromatography-tandem mass spectrometry. The relative bioavailability of SMEDDS was dramatically enhanced by an average of 9.8-fold compared with the suspension. Improved solubility and lymphatic transport may contribute to this enhanced bioavailability. Our studies highlight the promise of SMEDDS for the delivery of 25-OCH3-PPD via the oral route.
    International Journal of Nanomedicine 02/2014; 9(1):913-920. DOI:10.2147/IJN.S56894 · 4.38 Impact Factor

Publication Stats

187 Citations
89.97 Total Impact Points


  • 2007-2015
    • Shenyang Pharmaceutical University
      • • School of Traditional Chinese Materia Medica
      • • Department of Traditional Chinese Medicine
      Feng-t’ien, Liaoning, China
  • 2004
    • Northeast Normal University
      Hsin-ching, Jilin Sheng, China