Jixiu Zhang

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (5)17.35 Total impact

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    ABSTRACT: In order to determine how plant uptake of a sulfur-rich secondary metabolite, sinalbin, affects the metabolic profile of sulfur-deficient plants, gas chromatography time-of-flight mass spectrometry (GC–TOF-MS), in combination with liquid chromatography–mass spectrometry (LC–MS), was used to survey the metabolome of Arabidopsis seedlings grown in nutrient media under different sulfur conditions. The growth media had either sufficient inorganic sulfur for normal plant growth or insufficient inorganic sulfur in the presence or absence of supplementation with organic sulfur in the form of sinalbin (p-hydroxybenzylglucosinolate). A total of 90 metabolites were identified by GC–TOF-MS and their levels were compared across the three treatments. Of the identified compounds, 21 showed similar responses in plants that were either sulfur deficient or sinalbin supplemented compared to sulfur-sufficient plants, while 12 metabolites differed in abundance only in sulfur-deficient plants. Twelve metabolites accumulated to higher levels in sinalbin-supplemented than in the sulfur-sufficient plants. Secondary metabolites such as flavonol conjugates, sinapinic acid esters and glucosinolates, were identified by LC–MS and their corresponding mass fragmentation patterns were determined. Under sinalbin-supplemented conditions, sinalbin was taken up by Arabidopsis and contributed to the endogenous formation of glucosinolates. Additionally, levels of flavonol glycosides and sinapinic acid esters increased while levels of flavonol diglycosides with glucose attached to the 3-position were reduced. The exogenously administered sinalbin resulted in inhibition of root and hypocotyl growth and markedly influenced metabolite profiles, compared to control and sulfur-deficient plants. These results indicate that, under sulfur deficient conditions, glucosinolates can be a sulfur source for plants. This investigation defines an opportunity to elucidate the mechanism of glucosinolate degradation in vivo.Graphical abstractGas chromatography time-of-flight mass spectrometry (GC–TOF-MS) in combination with liquid chromatography–mass spectrometry (LC–MS) was used to survey the metabolome of Arabidopsis seedlings in response to sulfur deficiency and sinalbin supplements.Highlights► Metabolite profiling of Arabidopsis seedlings in response to sulfur deficiency and sinalbin supplement. ► Identification and quantification of metabolites by GC–TOF-MS and LC–MS. ► Investigation of mass fragmentation patterns of flavonol conjugates, sinapinic acid esters and glucosinolates. ► Comparison of metabolite profiles by principal component analysis. ► Interpretation of metabolite differences via metabolic pathways.
    Phytochemistry 10/2011; 72(14-15):1767-1778. DOI:10.1016/j.phytochem.2011.06.002 · 3.35 Impact Factor
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    ABSTRACT: The metabolic responses of Arabidopsis thaliana to cadmium exposure was characterized in this study. A. thaliana was cultivated in medium contaminated with different cadmium concentrations (0, 5 and 50microM, respectively) for 2weeks. Metabolite analyses were performed using gas chromatography-mass spectrometry. More than 80 metabolites characterized by retention time indices and specific mass fragments were identified. The levels of carbohydrates, organic acids, amino acids, and other stress-responsive metabolites changed under cadmium stress. Treated plants showed increased levels of Ala, beta-ala, Pro, Ser, putrescine, Suc and other metabolites with compatible solute-like properties, notably 4-aminobutyric acid, glycerol, raffinose and trehalose, compared to control (untreated) plants. Studies indicated that concentrations of antioxidants (alfa-tocopherol, campesterol, beta-sitosterol and isoflavone) also increased significantly. These results confirm the important role of antioxidant defences in the mechanisms of plant-resistance to cadmium stress. Our results suggested that metabolic profiling is a powerful tool that can rapidly classify environmentally modified plants and simplify the process of cadmium-stress responses. These data will be helpful for better understanding of mechanisms of plant adaptation to cadmium stress at the metabolite level.
    Chemosphere 02/2010; 78(7):840-5. DOI:10.1016/j.chemosphere.2009.11.045 · 3.50 Impact Factor
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    ABSTRACT: The influence of cadmium on growth, cadmium accumulation, composition, and content of glucosinolates was investigated in Arabidopsis thaliana after 4weeks of growth in hydroponics. Accumulation of 3,820 and 321μg Cd g−1 dry weight in the roots and leaves of A. thaliana, respectively, exposed to 50μM Cd. Cadmium treatment significantly decreased the total concentration of glucosinolates both in the leaves and roots. Cd-induced alteration of total glucosinolate content in the roots was mainly due to the decrease of indolyl-glucosinolates. In the Cd treatment leaves, significant decreases were, respectively, detected for glucoibervirin and 4-methoxyglucobrassicin (P < 0.01), while other glucosinolate levels did not decrease significantly. In response to cadmium, the three indolyl-glucosinolates all showed significant decreases in the roots. The distinctive influence of cadmium on glucosinolate profiles in Cd-sensitive A. thaliana may be of great ecological importance, decreasing the resistance to phytophage attack. Taken together, our data is discussed in relation to jasmonic acid and salicylic acid as possible molecules that modulate the alteration of glucosinolate profiles in response to cadmium. The similar effects of Cd treatment on the levels of individual glucosinolates in leaves and roots were observed at higher-concentration cadmium treatment (100μM Cd).
    Water Air and Soil Pollution 01/2009; 200(1):109-117. DOI:10.1007/s11270-008-9897-3 · 1.69 Impact Factor
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    ABSTRACT: Uniform monodisperse magnesium oxide microspheres with a high surface area have been prepared by a facile seed-induced precipitation. By characterizing these particles with scanning electron microscopy and N(2) physisorption techniques, the results demonstrate that these magnesium oxide microspheres have an average particle diameter of 9.5 microm, a specific surface area of 211.7 m(2)g(-1), a total pore volume of 0.76 mL g(-1), and an average pore diameter of 143 A. The chromatographic properties of these microspheres have been investigated in normal-phase mode for the separation of various basic compounds including aniline, quinoline, and pyridine derivatives. In contrast to conventional silica, the magnesium oxide particles exhibit unique selectivity and retention property for the separation of the tested basic compounds, and these microspheres are promising as an alternative new packing material for high-performance liquid chromatography.
    Journal of Chromatography A 10/2007; 1165(1-2):116-21. DOI:10.1016/j.chroma.2007.07.072 · 4.26 Impact Factor
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    ABSTRACT: A self-assembled spherical-like basic magnesium carbonate (Mg5(CO3)4(OH)2·4H2O) with diameters of 15−17 μm was synthesized in an aqueous solution system without using any organic additives during the precipitation process. Reaction parameters such as reaction time, stirring time, and reaction temperature were found to be important in controlling the final morphology of Mg5(CO3)4(OH)2·4H2O. By the investigation of the particles from time-dependent experiments, scanning electron microscopy observations and Fourier transform infrared spectra indicate that the formation of these spherical-like Mg5(CO3)4(OH)2·4H2O is via a complex process, in which the agglomerates built by many fine grains are first produced after the “burst” mixture of K2CO3 and Mg(NO3)2 solutions. Then the agglomerates tend to assemble into needlelike or other similar particles, and the latter, due to their unstable properties from the thermodynamic point of view, will transfer into sheetlike particles, which further assemble into spherical-like particles. During such a process, their corresponding compositions also change from (MgCO3)0.8(Mg(OH)2)0.2·1.3H2O to MgCO3·xH2O, then to Mg5(CO3)4(OH)2·4H2O. In addition, the stirring time and reaction temperature were found to play a comparative role in determining the final morphology of Mg5(CO3)4(OH)2·4H2O, and the morphology transition from spherical-like to nest-like, then to layered particles, as well as the decrease of particle size, can be well obtained by carefully adjusting the stirring time (0.5−4 min) of the initial solution and reaction temperature (333−363 K).
    Crystal Growth & Design 01/2007; 7(2). DOI:10.1021/cg060544y · 4.56 Impact Factor