V V Andrushkevich

Publications (3)0 Total impact

• Article: [Nitric oxide metabolites and their significance in pathogenesis of bronchial asthma].

  O V Kozina, L M Ogorodova, V V Andrushkevich, A E Sazonov, I V Petrova, V A Egorov, E V Komiakova, N N Chusova, M S Iusubov
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  ABSTRACT: To study the possible potential of nitric oxide (NO) in the processes of atopic inflammation, the authors evaluated the intensity of nitrosyling and oxidative stresses in the bronchoalvelar lavage fluid and expired air condensate of patients with bronchial asthma (BA). Chronic inflammation was shown to result in an increase in the processes of nitration and oxidation in worsening BA and to reliably correlate with airway NO-producing functions, by explaining the pathological effects of NO due to the formation of 3-nitrotyrosine and malonic dialdehyde with the existing imbalance in NO metabolism, by intensifying nitrosylating stress. In the authors' opinion, nitrosothiols that are required as a NO donor may be rapidly destroyed or virtually do not form so the peroxynitrite-nitrosothiol ratio may predetermine the final effects of NO.
  Klinicheskaia laboratornaia diagnostika 07/2008;
• Article: [Definition of parameters of the condition of oxidizing stress in smooth muscle cells under influence of exogenous nitroso-glutatyon in vitro].

  L V Kapilevich, A V Nosarev, E Iu D'iakova, V V Andrushkevich, A K Nasedkina, O L Nosareva, K V Davlet'iarova, L M Ogorodova, I V Kovalev, M B Baskakov, M A Medvedev
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  ABSTRACT: Influence of exogenous nitroso-glutatyon on intensity of oxidizing processes in smooth muscles of colon and bronchial tubes in intact and atopic sensitised porpoises (guinea pigs) was studied. In sensitised porpoises, antioxidant protection has been initially reduced against the background of increased maintenance of products of oxidizing that reflects a picture of oxidizing damage and can be associated with an inflammatory process. In incubation with nitroso-glutatyon, a decrease in activities of syperoxiddismutase and catalase is marked and, in sensitised animals, this effect has been expressed to a lesser degree. Syperoxiddismutase and catalase are antioxidant for the enzymes participating in protection of cells from free-radical damage. A dose-dependence decrease in activity catalase and syperoxiddismutase is defined by a parity of the enzymes participating in disintegration of nitrosoglutatyon and the enzymes which have kept antioxidant activity.
  Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk 09/2007; 93(8):898-904.
• Article: [Enzymes of purine metabolism of lymphocytes and eosinophiles in bronchial asthma].

  V V Andrushkevich, G A Sukhanova, L I Volkova, D L Chukhnova
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  ABSTRACT: The activity of the enzyme purine metabolism (5'-nucleotidase, adenosine deaminase, xanthine oxidase and the content of uric acid, i.e. the final product of the purine metabolism) were determined in lymphocytes, eosinophiles and blood serum of patients with bronchial asthma. The activity of 5'-nucleotidase in lymphocytes, eosinophiles and blood serum of patients with bronchial asthma was found to be reduced by 5-7-% as compared with the controls. The activity of adenosine deaminase in lymphocytes, eosinophiles and blood serum was found to be reduced in a majority of patients. An increased activity of adenosine deaminase was higher in lymphocytes and blood serum of 38% of the examined patients. The activity of xanthine oxidase in lymphocytes and eosinophiles of patients exceeded by 2.4 and 1.7 times the control value, respectively. The content of urine acid was found to be increased, in bronchial asthma, by 2.7 times. Consequently, the balance of enzyme reactions of the purine metabolism is impaired in bronchial asthma, and there is an accumulation of urine acid.
  Klinicheskaia laboratornaia diagnostika 05/2003;