[Show abstract][Hide abstract] ABSTRACT: Peptide nucleic acid (PNA) is known to bind with extraordinarily high affinity and sequence-specificity to complementary nucleic acid sequences and can be used to suppress gene expression. However, effective delivery into cells is a major obstacle to the development of PNA for gene therapy applications. Here, we present a novel method for the in vitro delivery of antigene PNA to cells. By using a nucleocapsid protein derived from Simian virus 40, we have been able to package PNA into pseudovirions, facilitating the delivery of the packaged PNA into cells. We demonstrate that this system can be used effectively to suppress gene expression associated with multidrug resistance in cancer cells, as shown by RT-PCR, flow cytometry, Western blotting, and cell viability under chemotherapy. The combination of PNA with the SV40-based delivery system is a method for suppressing a gene of interest that could be broadly applied to numerous targets.
PLoS ONE 03/2011; 6(3):e17981. DOI:10.1371/journal.pone.0017981 · 3.23 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Peptide nucleic acids are a class of nondegradable oligonucleotide mimics that can be used as probes for nucleic acid sequences
and could convey the necessary stability to be a diagnostic tool for use in a resourcelimited setting. In this review, there
is a brief introduction to the field of peptide nucleic acids and their potential benefits as probes for DNA and RNA sequences,
followed by highlights of ways by which peptide nucleic acids could benefit a number of established diagnostic tools for human
immunodeficiency virus detection.
The Journal of Infectious Diseases 04/2010; 201 Suppl 1(s1):S42-5. DOI:10.1086/650389 · 6.00 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Technologies for genomic detection most commonly use DNA probes to hybridize to target sequences. To achieve required sensitivity, the use of PCR to amplify target sequences has remained standard practice in many labs. Direct detection methods that eliminate the requirement for a PCR step could afford faster and simpler devices that can be used outside of a laboratory. We have developed a sandwich-hybridization DNA detection system in which the DNA detection probes are replaced with a class of synthetic nucleic acid mimics called peptide nucleic acids (PNAs). There are numerous advantages to using PNA instead of DNA probes in hybridization assays, including complete resistance to degradation by enzymes, increased sequence specificity to complementary DNA, and higher stability when bound with complementary DNA. In this communication, several different strategies are described to improve and fine-tune the properties of PNA so that anthrax DNA can be detected at a 10 zmol limit. Key to the success of these strategies is the ability to introduce chemical modifications into the PNA to improve thermal stability and to increase the number of biotin groups to which a horseradish peroxidase-avidin conjugate can bind.
Journal of the American Chemical Society 08/2007; 129(27):8424-5. DOI:10.1021/ja072744j · 12.11 Impact Factor