Fei Ye

Publications (140)349.94 Total impact

• Article: Radial approach for patients with ST-segment elevation acute coronary syndrome: It is definitely the best access site.

  Yao-Jun Zhang, Bo Xu, Patrick W Serruys, Christos V Bourantas, Javaid Iqbal, Takashi Muramatsu, Ming-Hui Li, Fei Ye, Nai-Liang Tian, Hector M Garcia-Garcia, Shao-Liang Chen
  International journal of cardiology 05/2013; · 7.08 Impact Factor
• Article: Aberrant expression of signaling proteins in essential thrombocythemia.

  Wuhan Hui, Fei Ye, Wei Zhang, Congyan Liu, Miao Cui, Wei Li, Juan Xu, David Y Zhang
  [show abstract] [hide abstract]
  ABSTRACT: Dysregulated expression of signaling proteins may contribute to the pathophysiology of essential thrombocythemia (ET). This study aimed to characterize protein expression in ET and to correlate the dysregulated proteins with phenotypes and prognosis of ET patients. The expression of 128 proteins in peripheral blood neutrophils from 74 ET patients was assessed and compared with those from 29 healthy subjects and 35 polycythemia vera (PV) patients using protein pathway array. Fifteen proteins were differentially expressed between ET patients and normal controls. These dysregulated proteins were involved in the signaling pathways related with apoptosis and inflammation. Our results showed a significant overlap in protein expression between ET patients with JAK2V617F mutation and PV patients. In addition, nine proteins were associated with JAK2V617F mutation status in ET patients. Furthermore, estrogen receptor beta (ERβ) and Stat3 were independent risk factors for subsequent thrombosis during follow-up on multivariable analysis. Our study shows a broad dysregulation of signaling protein in ET patients, suggesting their roles in ET pathogenesis. The expression levels of ERβ and Stat3 could be promising predictors of subsequent thrombosis in ET patients.
  Annals of Hematology 05/2013; · 2.62 Impact Factor
• Article: Coronary bifurcation lesions treated with double kissing crush technique compared to classical crush technique: serial intravascular ultrasound analysis.

  Shou-Jie Shan, Fei Ye, Zhi-Zhong Liu, Nai-Liang Tian, Jun-Jie Zhang, Shao-Liang Chen
  [show abstract] [hide abstract]
  ABSTRACT: The double kissing (DK) crush technique is a modified version of the crush technique. It is specifically designed to increase the success rate of the final kissing balloon post-dilatation, but its efficacy and safety remain unclear. Data were obtained from the DKCRUSH-I trial, a prospective, randomized, multi-center study to evaluate safety and efficacy. Post-procedural and eight-month follow-up intravascular ultrasound (IVUS) analysis was available in 61 cases. Volumetric analysis using Simpson's method within the Taxus stent, and cross-sectional analysis at the five sites of the main vessel (MV) and three sites of the side branch (SB) were performed. Impact of the bifurcation angle on stent expansion at the carina was also evaluated. Stent expansion in the SB ostium was significantly less in the classical crush group ((53.81 ± 13.51)%) than in the DK crush group ((72.27 ± 11.46)%) (P = 0.04). For the MV, the incidence of incomplete crush was 41.9% in the DK group and 70.0% in the classical group (P = 0.03). The percentage of neointimal area at the ostium had a tendency to be smaller in the DK group compared with the classical group ((16.4 ± 19.2)% vs. (22.8 ± 27.1)%, P = 0.06). The optimal threshold of post-procedural minimum stent area (MSA) to predict follow-up minimum lumen area (MLA) < 4.0 mm(2) at the SB ostium was 4.55 mm(2), yielding an area under the curve of 0.80 (95% confidence interval: 0.61 to 0.92). Our data suggest that the DK crush technique is associated with improved quality of the final kissing balloon inflation (FKBI) and had smaller optimal cutoff value of post-procedural MSA at the SB ostium.
  Chinese medical journal 04/2013; 126(7):1247-51. · 0.86 Impact Factor
• Article: Comparison of Double Kissing Crush Versus Culotte Stenting for Unprotected Distal Left Main Bifurcation Lesions: Results From a Multicenter, Randomized, Prospective DKCRUSH-III Study.

  Shao-Liang Chen, Bo Xu, Ya-Ling Han, Imad Sheiban, Jun-Jie Zhang, Fei Ye, Tak W Kwan, Chitprapai Paiboon, Yu-Jie Zhou, Shu-Zheng Lv, [......], Shang-Yu Wen, Lang Hong, Rui-Yan Zhang, Hai-Chang Wang, Tie-Ming Jiang, Yan Wang, Fang Chen, Zu-Yi Yuan, Wei-Min Li, Martin B Leon
  [show abstract] [hide abstract]
  ABSTRACT: OBJECTIVES: The study aimed to investigate the difference in major adverse cardiac event (MACE) at 1-year after double kissing (DK) crush versus culotte stenting for unprotected left main coronary artery (UPLMCA) distal bifurcation lesions. BACKGROUND: DK crush and culotte stenting were reported to be effective for treatment of coronary bifurcation lesions. However, their comparative performance in UPLMCA bifurcation lesions is not known. METHODS: A total of 419 patients with UPLMCA bifurcation lesions were randomly assigned to DK (n = 210) or culotte (n = 209) treatment. The primary endpoint was the occurrence of a MACE at 1 year, including cardiac death, myocardial infarction, and target vessel revascularization (TVR). In-stent restenosis (ISR) at 8 months was secondary endpoint, and stent thrombosis (ST) served as a safety endpoint. Patients were stratified by SYNTAX (Synergy between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery) and NERS (New Risk Stratification) scores. RESULTS: Patients in the culotte group had significant higher 1-year MACE rate (16.3%), mainly driven by increased TVR (11.0%), compared with the DK group (6.2% and 4.3%, respectively; all p < 0.05). ISR rate in side branch was 12.6% in the culotte group and 6.8% in the DK group (p = 0.037). Definite ST rate was 1.0% in the culotte group and 0% in the DK group (p = 0.248). Among patients with bifurcation angle ≥70°, NERS score ≥20, and SYNTAX score ≥23, the 1-year MACE rate in the DK group (3.8%, 9.2%, and 7.1%, respectively) was significantly different to those in the culotte group(16.5%, 20.4%, and 18.9%, respectively; all p < 0.05). CONCLUSIONS: Culotte stenting for UPLMCA bifurcation lesions was associated with significantly increased MACEs, mainly due to the increased TVR. (Double Kissing (DK) Crush Versus Culotte Stenting for the Treatment of Unprotected Distal Left Main Bifurcation Lesions: DKCRUSH-III, a Multicenter Randomized Study Comparing Double-Stent Techniques; ChiCTR-TRC-00000151).
  Journal of the American College of Cardiology 02/2013; · 14.16 Impact Factor
• Article: A role for LRIG1 in the regulation of malignant glioma aggressiveness.

  Feng Mao, Baofeng Wang, Qungen Xiao, Guifa Xi, Wei Sun, Huaqiu Zhang, Fei Ye, Feng Wan, Dongsheng Guo, Ting Lei, Xiaoping Chen
  [show abstract] [hide abstract]
  ABSTRACT: The molecular mechanisms that drive the develop-ment and aggressive progression of malignant astrocytic tumors remain obscure. Recently, in the search for endo-genous negative regulators of EGF receptor, LRIG1 was cloned and characterized as a putative tumor suppressor gene often downregulated in various human tumors, including astrocytic tumors. Although several studies have implicated the function of LRIG1 in the inhibition of tumorigenesis, its precise role and potential underlying mechanisms remain obscure. Therefore, we generated a full-length expression vector to overexpress LRIG1 in the U251 malignant glioma cell line. Introduction of exogenous LRIG1 into glioma cells inhibited cell proliferation manifested by MTT and soft agar clone assay in vitro and subcutaneously tumor xenografts. On the other hand, LRIG1 overexpression inhibited glioma growth by significantly changing the expression pattern of cyclins, resulting in delayed cell cycle. Employing transwell invasion and wound scratch assay and gelatin zymography, LRIG1 inhibited U-251 MG cell invasion and migration by attenuating MMP2 and MMP9 production. Under ligand-stimulated conditions, p-ERK levels did not change, whereas p-AKT levels were inhibited in cells with LRIG1 upregulation, indicating that LRIG1 exerts more inhibiting effects on the PI3K/AKT pathway. Our findings suggest that LRIG1 restricted glioma aggressiveness by inhibiting cell proliferation, migration and invasion. Restoration of LRIG1 to glioma cells could offer a novel therapeutic strategy.
  International Journal of Oncology 01/2013; · 2.40 Impact Factor
• Article: [Differentiation treatment by all-trans retinoic acid reduces stemness of glioma stem cells].

  Suo-Jun Zhang, Feng Wan, Feng Hu, Rui-Fan Xie, Ying Wang, Bao-Feng Wang, Fei Ye, Dong-Sheng Guo, Ting Lei
  [show abstract] [hide abstract]
  ABSTRACT: To explore the effects of all-trans retinoic acid (ATRA) on glioma stem cell phenotype. The glioma stem cell (GSC) from surgically resected human glioma specimens were isolated and enriched by neurosphere assay and then its differentiation was induced with all-trans retinoic acid (ATRA, 1 µmol/L) for 1 week. Markers were determined by flow cytometry, Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Side population cells were analyzed by flow cytometry. Growth characteristics were detected by neurosphere formation assay and cell cycle analysis. GSC and the differentiated cells (1×10(5)) were implanted stereotactically and intracranially into the Balb/c nude mice to compare the survival time. All data were analyzed with the SPSS software version 17.0. ATRA potently induced the differentiation of GSC and reduced glioma stem cell phenotype. And there were an elevated expression of glial fibrillary acidic protein (GFAP) and a reduced expression of such stem cell makers as CD133 and Nestin. The side population rate decreased. ATRA inhibited the neurosphere formation of GSC and induced the arrest of cell growth. ATRA could prolong the survival time. GSC may be differentiated efficiently by ATRA. The stemness of GSC decreases obviously after the differentiation of ATRA and the survival time is prolonged. Thus ATRA may be applied for targeted therapies of glioma stem cell.
  Zhonghua yi xue za zhi 01/2013; 93(1):19-22.
• Article: Comparison of long-term clinical outcome between patients with chronic versus acute type B aortic dissection treated by implantation of a stent graft: a single-center report.

  Shao-Liang Chen, Jian-Cheng Zhu, Xiao-Bo Li, Fei Ye, Jun-Jie Zhang, Zhi-Zhong Liu, Nai-Liang Tian, Song Lin, Cheng-Yu Lv
  [show abstract] [hide abstract]
  ABSTRACT: Stent grafting for treatment of type B aortic dissection has been extensively used. However, the difference in the long-term clinical outcome between patients with chronic versus acute type B aortic dissection remains unknown. This study aimed to analyze the difference in long-term clinical outcome after endovascular repair for patients with chronic (≥2 weeks) versus acute (<2 weeks) type B aortic dissection. Between May 2000 and June 2011, a total of 174 patients with type B aortic dissection (56 chronic, 118 acute) treated by endovascular repair were studied prospectively. Follow-up three-dimensional computed tomography scanning and aortoangiography were scheduled at 3-6 months after the index procedure. Propensity score matching was used to compare the difference in the endpoint between the two groups. The procedure-related event rate was 18.6% in the acute group and 5.4% in the chronic group (P = 0.021), but this difference became nonsignificant after propensity score matching. At the end of follow-up (mean 2.49 years), overall and aorta-related mortality was 11.0% and 7.6%, respectively, in the acute group, and was not significantly different from that in the chronic group (3.6% and 3.6%, P = 0.148 and P = 0.506, respectively). Both false and true lumina showed significant remodeling over time, with >93% complete false-lumen thrombosis. Untreated tear and type I endoleak were predictors of clinical events during follow-up. Comparable long-term clinical results were achieved in patients with chronic or acute type B aortic dissection after implantation of a stent graft.
  Patient Preference and Adherence 01/2013; 7:319-27. · 1.14 Impact Factor
• Article: Effect of genomic instability and mutations on the signaling pathways in colon cancer cells

  Miao Cui, Fei Ye, Bo Jiang, Peng Deng, Xinying Wang, Yong Jiang, David Yong Zhang
  [show abstract] [hide abstract]
  ABSTRACT: Microsatellite instability (MSI) is present in about 15% of colorectal cancers and plays critical roles in the development and progression of these cancers. The goal of this study is to determine the global effect of microsatellite instability on the signaling pathways and network in colon cancer cells. We profiled the expression and phosphorylation of 110 proteins in six colon cancer cell lines by using Protein Pathway Array. The pathways and network constituted by these proteins were identified by using Ingenuity Pathway Analysis. Our results showed that 25 proteins and phosphoproteins change more than 1.5-fold between MSI and microsatellite stable (MSS) cells. Sixteen major pathways were affected in MSI cells, including p53 and 14-3-3ß pathways, with p53 and HGF being the most important pathways. Finally, although the EGFR/K-RAS/MEK pathway was not affected in MSI cells, collateral pathways such as the p70S6K and p90RSK pathways were activated in MSI cells. Thus, suppression of the p53 pathway and activation of the HGF pathway in MSI cells may be critical in the tumorigenesis of MSI colorectal cancer.
  Frontiers in bioscience (Elite edition) 01/2013; E5:574-582.
• Article: Diagnostic accuracy of quantitative angiographic and intravascular ultrasound parameters predicting the functional significance of single de novo lesions.

  Shao-Liang Chen, Bo Xu, Jack B Chen, Tian Xu, Fei Ye, Jun-Jie Zhang, Tak W Kwan, Nai-Liang Tian, Zhi-Zhong Liu, Song Lin
  [show abstract] [hide abstract]
  ABSTRACT: OBJECTIVES: The current study aimed at determining the best cutoff value of angiographic and intravascular ultrasound (IVUS) parameters for defining fractional flow reserve (FFR) <0.8 in patients with single coronary artery lesion. BACKGROUND: The correlation between angiographic or IVUS variables and FFR in patients with single coronary artery lesions has not been studied yet. METHODS: Quantitative coronary analysis and IVUS and FFR measurements were used in 323 patients with a single lesion. The best angiographic and IVUS cutoff values and their predictive value for FFR<0.8 were compared using area under the receiver-operator characteristic curve (AUC). RESULTS: FFR<0.8 was in 54.2%. Minimal lumen area (MLA), plaque burden (PB), lesion length (LL) and lesion at left anterior descending artery (LAD) were four predictors of FFR<0.8. LL had less value in predicting FFR<0.8. The cutoff values of PB and MLA for FFR<0.8 were 72.7% and 2.97mm(2). MLA and PB had similar high diagnostic value for vessel size≥3mm (cutoff values: 3.02mm(2) and 80.7%), proximal LAD lesion (cutoff values: 3.04mm(2) and 76.5%) and unstable angina (2.82mm(2) and 71.9%). Combination of MLA (2.82mm(2)) and PB (80.6%) had increased diagnostic value for distal LAD lesion. Only PB (71%) had higher diagnostic value for diabetic patients. MLA and PB could not predict FFR<0.8 for vessel size<3mm, and non-LAD lesion. CONCLUSION: Best cutoff value of MLA and PB for FFR<0.8 in patients with a single lesion is patient-, vessel size- and lesion location-oriented. PB has strengthened diagnostic accuracy for diabetic patients.
  International journal of cardiology 12/2012; · 7.08 Impact Factor
• Article: Optimized quantitative angiographic and intravascular ultrasound parameters predicting the functional significance of single de novo lesions in the left anterior descending artery.

  Tak W Kwan, Song Yang, Bo Xu, Jack Chen, Tian Xu, Fei Ye, Jun-Jie Zhang, Nai-Liang Tian, Zhi-Zhong Liu, Shao-Liang Chen
  [show abstract] [hide abstract]
  ABSTRACT: The correlation between angiographic or intravascular ultrasound (IVUS) variables and fractional flow reserve (FFR) in patients with single left anterior descending artery (LAD) lesion has not been studied. The current study aimed at determining the best cutoff value of angiographic and IVUS parameters for defining FFR < 0.80 in patients with LAD lesion. Quantitative coronary analysis, IVUS and FFR measurements were undergone in 169 patients with single LAD lesion. The best angiographic and IVUS cutoff value and their predictive value for FFR < 0.80 were compared using area under the receiver-operator characteristic curve (AUC) in overall patients or in subgroups stratified by lesion sites. FFR < 0.80 was found in 99 lesions (58.6%). Minimal lumen area (MLA), and plaque burden (PB) were two predictors of FFR < 0.80. Lesion length had less value in predicting FFR < 0.80. The cutoff value of PB and MLA for FFR < 0.80 was 75.4% and 3.03 mm(2). MLA and PB had similar high diagnostic value for proximal (cutoff value 3.04 mm(2) and 76.5%) and distal LAD lesion (2.82 mm(2) and 80.6%). Combination of MLA (2.82 mm(2)) and PB (80.6%) had increased diagnostic value for distal LAD lesion. MLA and plaque burden had equivalent diagnostic value for FFR < 0.80 when lesion localized in LAD. The predictive value of combination of MLA and plaque burden for distal LAD lesion was strengthened.
  Chinese medical journal 12/2012; 125(23):4249-53. · 0.86 Impact Factor
• Article: Five-year clinical follow-up of unprotected left main bifurcation lesion stenting: one-stent versus two-stent techniques versus double-kissing crush technique.

  Shao-Liang Chen, Yaojun Zhang, Bo Xu, Fei Ye, Junjie Zhang, Nailiang Tian, Zhizhong Liu, Xuesong Qian, Shiqing Ding, Feng Li, Aiping Zhang, Yueqiang Liu, Song Lin
  [show abstract] [hide abstract]
  ABSTRACT: Aims: The present study aimed to compare the long-term (five-year) safety and efficacy between the one-stent, two-stent and double-kissing (DK) crush strategies, utilising drug-eluting stents, for unprotected left main coronary artery (ULMCA) bifurcation lesions. Methods and results: Between March 2004 and April 2007, 633 consecutive patients with ULMCA bifurcation lesions (232 in the one-stent group and 401 in the two-stent group) were prospectively enrolled. The primary endpoint was the occurrence of major adverse cardiac events (MACE), a composite of cardiac death, myocardial infarction (MI), and target vessel revascularisation (TVR), at five-year follow-up. Patients in the the two-stent group were classified as DK crush (n=155) and other two-stent techniques (culotte, T-stenting, kissing stenting and classical crush, n=246). Forty-seven (16.8%) patients in the one-stent group crossed over to the two-stent group. The one-stent group was associated with an increased incidence of MI compared to the two-stent approach (10.5% vs. 5.5%, p=0.025). The crude rate of MACE at five years was 28.0% in the one-stent group and 28.4% in the two-stent group (p=0.927). DK crush was associated with a significantly decreased five-year MACE compared to the other two-stent approaches or the one-stent approach (DK crush: 14.8% vs. other two-stent approaches: 37.0%, one-stent approach: 28.0%, p<0.001). The main benefit of DK crush primarily appeared to be secondary to a reduction in TVR (7.7% vs. 30.5% vs. 18.1%, p<0.001). By Cox regression analyses, the non-DK crush two-stent technique, a high SYNTAX Score (≥33) or New Risk Stratification (NERS) score (>20), and incomplete revascularisation were shown to be independent predictors of MACE at five-year follow-up. Conclusions: With distal left main true bifurcations, the two-stent technique (excluding DK crush) is an independent predictor of long-term MACE. DK crush is associated with more favourable long-term clinical outcomes. Confirmation of these findings is required from randomised controlled trials.
  EuroIntervention: journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology 11/2012; 8(7):803-14. · 3.29 Impact Factor
• Article: Improvement of cathode performance on Pt-CeOx by optimization of electrochemical pre-treatment condition for PEFC application.

  Keisuke Fugane, Toshiyuki Mori, Ding Rong Ou, Pengfei Yan, Fei Ye, Hideki Yoshikawa, John Drennan
  [show abstract] [hide abstract]
  ABSTRACT: Pt-CeOx/C electrocatalysts for the improvement of oxygen reduction reaction (ORR) activity on cathode were prepared by a combined process of precipitation and co-impregnation methods. The Pt-CeOx/C electrocatalysts pre-treated by the optimized electrochemical conditioning process showed high ORR activity as compared with home-made Pt/C electrocatalyst. Also, it showed a high stability in the cyclic voltammetry (CV) test up to 1000 cycles into 0.5M H2SO4 aqueous solution. Based on the data of cyclic voltammogram of 30 cyclic sweeps, X-ray photoelectron spectroscopy, electron energy loss spectroscopy, high resolution transmission electron microscope image and selected area electron diffraction analysis, it is concluded that the Pt-CeOx hetero-interface involving defect cluster which is formed by using optimized electrochemical pre-treatment condition on Pt in Pt-CeOx/C electro-catalyst contributes to the promotion of ORR activity and retention of its stability in long CV test up to 1000 cycles.
  Langmuir 10/2012; · 4.19 Impact Factor
• Article: Downregulation of LRIG1 expression by RNA interference promotes the aggressive properties of glioma cells via EGFR/Akt/c-Myc activation.

  Ruifan Xie, Hai Yang, Qungen Xiao, Feng Mao, Suojun Zhang, Fei Ye, Feng Wan, Baofeng Wang, Ting Lei, Dongsheng Guo
  [show abstract] [hide abstract]
  ABSTRACT: The LRIG1 [leucine-rich repeats and immunoglobulin-like domains (LRIG)] gene is not universally downregulated in human cancers, and its role in tumorigenesis and the development of glioma has not been well addressed. In this study, we used short hairpin RNA (shRNA)-triggered RNA interference (RNAi) to block LRIG1 gene expression in the GL15 human glioma cell line. Specific downregulation of LRIG1 by shRNA resulted in significantly enhanced capabilities of proliferation, inhibition of apoptosis and invasion in the GL15 cells. LRIG1 repression induced marked activation of epidermal growth factor receptor (EGFR), protein kinase B (Akt) and c-Myc signaling molecules. Our results demonstrated that RNAi against LRIG1 may effectively downregulate LRIG1 gene expression. LRIG1 functions as a tumor suppressor in the pathogenesis of glioma via EGFR/Akt/c-Myc activation.
  Oncology Reports 10/2012; · 1.84 Impact Factor
• Article: Comparison of paclitaxal vs. sirolimus eluting stents with bio-degradable polymer for the treatment of coronary bifurcation lesions: subgroup analysis from DKCRUSH-I and DKCRUSH-II studies.

  Shao-Liang Chen, Gary Mintz, Teguh Santoso, Jun-Jie Zhang, Fei Ye, Ya-Wei Xu, Qiang Fu, Jing Kan, Chitprapai Paiboon, Yong Zhou, Shi-Qin Ding, Tak W Kwan
  [show abstract] [hide abstract]
  ABSTRACT: The difference in clinical outcome between paclitaxal-eluting stents (PES) and sirolimus-eluting stents with bio-degradable polymer (SES-BDP) for bifurcation lesions remains unclear. The present study aimed to investigate the one-year clinical outcome after DK crush stenting using PES (Taxus(TM)) vs. SES-BDP (Excel(TM)) from our database. A total of 275 patients (90 from the DKCRUSH-I and 185 from the DKCRUSH-II study) were studied. The primary endpoint was the occurrence of major adverse cardiac events (MACE) at 12 months; including cardiac death, myocardial infarction (MI), or target vessel revascularization (TVR). The rate of binary restenosis and stent thrombosis served as secondary endpoints. At follow-up, minimal luminal diameter (MLD) in the Taxus group was (2.11 ± 0.66) mm, with resultant increased target lesion revascularization (TLR) 12.2% and TVR 14.4%, significantly different from the Excel group; (2.47 ± 0.56) mm, P < 0.001, 3.2%, P = 0.006, 4.9%, P = 0.019, respectively. As a result there was a significant difference in MACE between the Taxus (20.0%) and Excel (10.3%, P = 0.038) groups. Overall stent thrombosis was monitored in 11 patients (4.0%), with five in the Excel group (2.7%) and six in the Taxus group (6.7%). All stent thrombosis in the Excel group was classified as early, and all were defined as late in the Taxus group. The Excel stent had lower rate of stent thrombosis, TLR, TVR, and composite MACE at 12-month after an indexed stenting procedure, compared to the Taxus stent.
  Chinese medical journal 10/2012; 125(19):3382-7. · 0.86 Impact Factor
• Article: Protein predictive signatures for lymph node metastasis of gastric cancer.

  Wei Li, Fei Ye, Daguang Wang, Xuan Sun, Weihua Tong, Guodong Lian, Jing Jiang, Jian Suo, David Y Zhang
  [show abstract] [hide abstract]
  ABSTRACT: Lymph node status remains one of most crucial indicators of gastric cancer prognosis and treatment planning. Current imaging methods have limited accuracy in predicting lymph node metastasis. We sought to identify protein markers in primary gastric cancer and to define a risk model to predict lymph node metastasis. The Protein Pathway Array (PPA) (initial selection) and Western blot (confirmation) were used to assess the protein expression in a total of 190 freshly frozen gastric cancer samples. The protein expression levels were compared between samples with lymph node metastasis (n=73) and those without lymph node metastasis (n=57) using PPA. There were 27 proteins differentially expressed between lymph node positive samples and lymph node negative samples. Five proteins (Factor XIII B, TFIIH p89, ADAM8, COX-2 and CUL-1) were identified as independent predictors of lymph node metastasis. Together with vascular/lymphatic invasion status, a risk score model was established to determine the risk of lymph node metastasis for each individual gastric cancer patient. The ability of this model to predict lymph node metastasis was further confirmed in a second cohort of gastric cancer patients (33 with and 27 without lymph node metastasis) using Western blot. This study indicated that some proteins differentially expressed in gastric cancer can be selected as clinically useful biomarkers. The risk score model is useful for determining patients' risk of lymph node metastasis and prognosis.
  International Journal of Cancer 09/2012; · 5.44 Impact Factor
• Article: Intravascular ultrasound-guided systematic two-stent techniques for coronary bifurcation lesions and reduced late stent thrombosis.

  Shao-Liang Chen, Fei Ye, Jun-Jie Zhang, Nai-Liang Tian, Zhi-Zhong Liu, Teguh Santoso, Yu-Jie Zhou, Tie-Ming Jiang, Shang-Yu Wen, Tak W Kwan
  [show abstract] [hide abstract]
  ABSTRACT: BACKGROUND: The effects of intravascular ultrasound (IVUS)-guided complex approaches using drug-eluting stents (DES) for coronary bifurcation lesions on clinical outcomes has not yet been studied in detail. OBJECTIVE: Our objective was to analyze the difference in one-year outcomes following two-stent techniques involving implantation of DES for coronary bifurcation lesions between IVUS-guided and angiography-guided groups. METHODS: From May 26, 2007 to March 24, 2010, 628 patients received two-stent techniques (324 in the IVUS-guided group and 304 angiography-guided) and were prospectively studied. We compared major adverse cardiac events (MACE, including cardiac death, stent thrombosis [ST], myocardial infarction [MI] and target lesion/vessel revascularization) at 12-months follow-up, before and after adjusting for propensity score matching. RESULTS: At 12-months after the indexed procedure, patients in the angiography-guided group had significantly increased in-stent restenosis. Compared to the angiography-guided group, the IVUS-guided group had a significantly lower overall unadjusted ST rate (1.2% vs. 6.9%, p<0.001), definite ST (0.6% vs. 5.3%, p<0.001), late ST (0.6% vs. 4.3%, p=0.003), MI (4.6% vs. 8.9%, p=0.038) and cardiac death (0.9% vs. 3.3%, p=0.049). By propensity score matching, 123 paired patients were matched. The late ST at 12-months follow-up was 0% in the IVUS-guided group vs. 4.9% in the angiography-guided group (p=0.029), resulting in significant differences in ST-elevation MI between the two groups (2.4% vs. 9.8%, p=0.030). CONCLUSIONS: The IVUS-guided two-stent technique was associated with significantly reduced late stent thrombosis, with a resultant reduction in ST-elevation MI. © 2012 Wiley Periodicals, Inc.
  Catheterization and Cardiovascular Interventions 08/2012; · 2.29 Impact Factor
• Article: Hemodynamic changes of fractional flow reserve after double kissing crush and provisional stenting technique for true bifurcation lesions.

  Fei Ye, Shao-Liang Chen, Jun-Jie Zhang, Zhong-Sheng Zhu, Jing Kan, Nai-Liang Tian, Song Lin, Zhi-Zhong Liu, Wei You, Hai-Mei Xu, Jing Xu
  [show abstract] [hide abstract]
  ABSTRACT: Fractional flow reserve (FFR)-guided percutaneous coronary intervention (PCI) is associated with fewer unfavorable events. However, the hemodynamic change in FFR after different stenting approaches for bifurcation lesions is still not fully studied. The aim of this study was to analyze the hemodynamic changes in FFR after double kissing (DK) crush and provisional side branch (SB) stenting (PS) for true coronary bifurcation lesions. Seventy-five patients with true bifurcated lesions were randomly divided into DK (n = 38) and PS (n = 37) groups. Additional SB stenting in the PS group was required if there was any pinched SB ostium > 70% stenosis, or ≥ type B dissection, or TIMI flow < grade 3. FFR at hyperemia in the main vessel (MV) and SB was measured prior- and post-stenting, and at 8 months follow-up. Baseline clinical, angiographic and lesion characteristics were matched well between the two groups, with the exception of the final kissing balloon inflation (FKBI, 100.0% in the DK vs. 83.8% in the PS group, P < 0.001). Baseline FFR was comparable between the DK and the PS groups, however, the acute gain and late loss of SB FFR at 8-month follow-up in the DK group were 0.18 ± 0.15 and -0.06 ± 0.11, compared to 0.12 ± 0.18 (P = 0.044) and -0.002 ± 0.07 (P = 0.037) in the PS group, respectively. MV FFR post-stenting > 0.94 was seen in about 40% of patients. There was no significant difference in the clinical events at 1-year follow-up between the two groups. DK crush was associated with improved acute gain and late loss of SB FFR. The lower rate of FFR > 0.94 after stenting underscored the further improvement of stenting quality.
  Chinese medical journal 08/2012; 125(15):2658-62. · 0.86 Impact Factor
• Article: Human coronary collateral recruitment is facilitated by isometric exercise during acute coronary occlusion.

  Song Lin, Xiao Lu, Shaoliang Chen, Fei Ye, Junjie Zhang, Yulin Ma, Jianan Li
  [show abstract] [hide abstract]
  ABSTRACT: To determine whether isometric exercise increases collateral flow in remote ischaemic myocardium in acute coronary occlusion models of patients with coronary artery disease. A randomized controlled study. Sixty-five patients with 1-vessel coronary artery disease. Subjects were randomly assigned to either the isometric exercise group or non-exercise group. Patients in the exercise group performed isometric handgrip exercises (50% maximal voluntary contraction) during 1 min coronary balloon occlusion, while patients in the non-exercise group remained sedentary. The collateral flow index, heart rate, systolic blood pressure and diastolic blood pressure were determined prior to and following 1 min of coronary occlusion. In the exercise group, difference values for collateral flow index (after coronary occlusion before coronary occlusion) were significantly higher than those in the non-exercise group (0.04 standard deviation (SD) 0.05 vs 0.01 (SD 0.03), p < 0.01). Differences in heart rate, systolic blood pressure and diastolic blood pressure were also significantly higher than controls (heart rate 7.84 (SD 8.20) vs 0.88 (SD 3.96), p < 0.01; systolic blood pressure: 3.25 (SD 7.17) vs 1.88 (SD 6.21), p < 0.01; diastolic blood pressure 5.88 (SD 6.40) vs 1.5 (SD 6.22), p < 0.01). Isometric exercise in patients with coronary artery disease induced an increase in coronary collateral flow during acute vessel occlusion, which was significantly different from control occlusion.
  Journal of rehabilitation medicine: official journal of the UEMS European Board of Physical and Rehabilitation Medicine 06/2012; 44(8):691-5. · 1.88 Impact Factor
• Article: Coronary bifurcation angle from 3-D predicts clinical outcomes after stenting bifurcation lesions.

  Shao-Liang Chen, Teguh Santoso, Jun-Jie Zhang, Fei Ye, Ya-Wei Xu, Qiang Fu, Jing Kan, Chitprapai Paiboon, Yong Zhou, Shi-Qing Ding, Tak W Kwan
  [show abstract] [hide abstract]
  ABSTRACT: The predictive value of bifurcation angle (BA) for worse events after stenting bifurcation lesions remains to be unknown. The present study was to investigate the dynamic change of BA and clinical relevance for patients with coronary bifurcation lesions treated by drug-eluting stent (DES). BA was calculated by 3-D quantitative coronary analysis from 347 patients in DKCRUSH-II study. Primary endpoint was the occurrence of composite major adverse cardiac events (MACE) at 12-month, including cardiac death, myocardial infarction (MI) and target vessel revascularization (TVR). Secondary end points were the rate of binary restenosis and stent thrombosis at 12-month. Stenting was associated with the reduction of distal BA. The cut-off value of distal BA for predicting MACE was 60°. Distal BA in < 60° group had less reduction after stenting ((-1.96 ± 13.58)° vs. (-12.12 ± 23.58)°, P < 0.001); two-stent technique was associated with significant reduction of distal BA (Δ(-4.05 ± 14.20)°), compared to single stent group (Δ + 1.55 ± 11.73, P = 0.003); the target lesion revascularization (TLR), TVR and MACE rate was higher in one-stent group (16.5%, 19.0% and 21.5%), compared to two-stent group (3.8%, P = 0.002; 7.5%, P = 0.016; and 9.8%, P = 0.024), respectively. Among patients in ≥ 60° group, there were no significant differences in distal BA, stent thrombosis (ST), MI, MACE, death, TLR, TVR between one- and two-stent groups; after stenting procedure, there was only slight change of distal BA in left anterior descending (LAD)-left circumflex (LCX) subgroup (from (88.54 ± 21.33)° at baseline to (82.44 ± 31.72)° post-stenting), compared to either LAD-diagonal branch (Di), or LCX-obtuse marginal branch (OM), or RCA distal (RCAd) (all P < 0.001). Two-stent technique was associated with significant reduction of distal BA. DK crush stenting had reduced rate of MACE in patients in < 60° group, compared to one-stent technique.
  Chinese medical journal 06/2012; 125(12):2083-8. · 0.86 Impact Factor
• Article: Real Polymer-free sirolimus- and probucol-eluting versus biodegradable polymer sirolimus-eluting stents for obstructive coronary artery disease: DKPLUS-Wave 1, a multi-center, randomized, prospective trial.

  Shao-Liang Chen, Fei Ye, Jun-Jie Zhang, Jian-Jun Zou, Xue-Song Qian, Feng Li, Song Yang, Zhen Ge, Shou-Jie Shan, Xiao-Bo Li, Tian Xu, Jing Kan, Ling Lin, Ya-Ling Han
  [show abstract] [hide abstract]
  ABSTRACT: BACKGROUND: Polymer-free sirolimus-and probucol-eluting stents (Real Dual DES) is as effective as first-generation DES in treating coronary artery stenosis. It is unknown whether sirolimus-eluting stents containing biodegradable polymer (Excel) would be superior to real Dual DES. The present study aimed to investigate the difference in target vessel revascularization (TVR) at 12 months in patients with coronary artery disease treated by the implantation of Dual DES or Excel stents. METHODS: 346 patients with de novo coronary artery disease were recruited from 6 centers in China and randomly assigned to either the Dual DES or the Excel group. The primary endpoint was the occurrence of TVR at 12 months. The secondary endpoint was angiographic in-stent restenosis (ISR) and late lumen loss at 13 months. Stent thrombosis (ST) served as the safety endpoint. Dual anti-platelet therapy (DAPT) was prescribed for 6-month. RESULTS: Clinical follow-up at 12 months and repeat angiography at 13 months were available in 100% and >90% of patients, respectively. The ISR and in-stent late loss were significantly different between the Excel (3.1%, 0.09±0.11 mm) and the Dual DES (19.5%,0.36±0.32 mm, p<0.001, p<0.001, respectively) groups. The TVR(3.5%) in the Excel group was significantly less than in the Dual DES group (13.9%, p=0.001). The ST rate beyond 12 months in the Dual DES group was 0%, and this was 1.2% in the Excel group (p=0.499). CONCLUSIONS: The Excel stent was statistically superior to the Dual DES in terms of restenosis, late loss and TVR for long lesions. ©2012 Blackwell Publishing Ltd.
  Cardiovascular Therapeutics 05/2012; · 2.35 Impact Factor