Likai Wang

Publications (3)10.24 Total impact

• Article: Two methods for glass surface modification and their application in protein immobilization.

  Ming Qin, Sen Hou, Likai Wang, XiZeng Feng, Rui Wang, Yanlian Yang, Chen Wang, Lei Yu, Bin Shao, MingQiang Qiao
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  ABSTRACT: Protein immobilization is a crucial step in protein chip, biosensor, etc. Here, two methods to immobilize proteins on glass surface were analyzed, one is silanization method using 3-aminopropyltriethoxysilane (APTES), and the other is hydrophobin HFBI coating. The modified glass surfaces were characterized with X-ray photoelectron spectroscopy (XPS), water contact angle measurement (WCA) and immunoassay. The results of XPS and WCA illustrated that the surface property of glass can be changed by both the two methods. The following immunoassay using microcontact printing (microCP) verified that both methods could help protein immobilization effectively on glass slides. Compared with the amine treatment, it is concluded that hydrophobin self-assemblies is a simple and generic way for protein immobilization on glass slides, which has potential application in protein chips and biosensors.
  Colloids and Surfaces B Biointerfaces 12/2007; 60(2):243-9. · 3.46 Impact Factor
• Article: Label-free biosensor: a novel phage-modified Light Addressable Potentiometric Sensor system for cancer cell monitoring.

  Yunfang Jia, Ming Qin, Hongkai Zhang, Wencheng Niu, Xiao Li, Likai Wang, Xin Li, Yunpeng Bai, Youjia Cao, Xizeng Feng
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  ABSTRACT: Early diagnosis has become the most important factor influencing the cancer's curing efficiency in clinical medicine. Here we present a new way for detection of cancer markers and cancer cells based on phage-modified Light Addressable Potentiometric Sensor (phage-LAPS). Phages were immobilized on the Si(3)N(4) chip surfaces covalently. Using the back-illuminating system, this phage-LAPS is tested to detect human phosphatase of regenerating liver-3 (hPRL-3) in the concentrations of 0.04-400 nM, and the mammary adenocarcinoma cell (MDAMB231) in the concentrations of 0-105 mL. The maximum responding signal is about 10 and 60 microV, respectively. The results demonstrated that the system was more applicable to detection of cancer cell than that of cancer biomarkers. This work might show potential application in clinic assays of cancer and the study of phage-cell interaction.
  Biosensors and Bioelectronics 07/2007; 22(12):3261-6. · 5.60 Impact Factor
• Article: Microcontact printing of multiproteins on the modified mica substrate and study of immunoassays

  Likai Wang, Xizeng Feng, Sen Hou, Qilin Chan, Ming Qin
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  ABSTRACT: Microcontact printing (µCP) is an easy and efficient way of producing patterns of self-assembled monolayers (SAM) containing different functional groups. We have developed a simple and convenient µCP-based technique for the modification of a mica substrate with 3-aminopropyltriethoxysilane (APTES) and the micropatterning of proteins (chicken IgG and rabbit IgG) on the modified mica surface. Our approach provides a quick and easy way to produce protein patterns on solid surfaces. The printed immunoglobulin patterns were detected by exposing the substrate to solutions containing fluorescently labeled complementary anti-IgGs, and the formed immunoassays were studied using fluorescence microscopy. Copyright © 2005 John Wiley & Sons, Ltd.
  Surface and Interface Analysis 12/2005; 38(1):44 - 50. · 1.18 Impact Factor