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02/2013;
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ABSTRACT: We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrants exchanged among the samples. The number of alleles per locus ranged from 5 to 18 (10.8 on average). The observed polymorphism information content ranged from 0.172 to 0.891. Beside the lower efficiency to obtain SSR loci, the six microsatellites were polymorphic and sufficiently discriminant for the genetic studies of S. frugiperda; it allowed us to identify migrants with both NJ clustering and the Bayesian methods. These markers will be useful for molecular ecology studies of this highly polyphagous species in order to understand the processes that determine genetic differentiation in the complex agro-ecosystems that it infests and improve local integrated pest management practices.
Genetics and molecular research: GMR 01/2013; 12(1):370-80. · 1.18 Impact Factor
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ABSTRACT: Melipona interrupta manaosensis is an important pollinator in the Neotropics. This stingless bee is reared by locals for honey production and also for conservation
purposes. Eleven microsatellite markers were isolated from M. i. manaosensis and characterized in 32 individuals from the Meliponary of the Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brazil.
The number of alleles observed for each locus ranged from 2 to 5 (average 2.77). The polymorphism information content (PIC)
varied between 0.08 to 0.62 (average 0.34) and the discriminating power (D) ranged from 0.61 to 0.99 (average 0.85). The observed
heterozygosity (H
O) and the expected heterozygosity (H
E) ranged from 0.04 to 1.00 and 0.04 to 0.67, respectively. These microsatellites will be useful for future genetic studies
of M. i. manaosensis populations and correlated species contributing with Amazon biodiversity preservation.
KeywordsAmazon-Pollinators-Molecular markers-Genetic diversity-Conservation
Conservation Genetics Resources 05/2012; 2(1):27-30. · 0.49 Impact Factor
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ABSTRACT: In this study, we used microsatellite loci to estimate the outcrossing rate of Centrosema pubescens in open-pollinated populations of 10 progenies that each contained 20 genotypes. The multilocus outcrossing rate was 27%,
which suggested a mixed mating system with a predominance of autogamy. The single locus outcrossing rate was 13%. The difference
was 0.040, which indicated that only 4% of outcrossing occurs in related individuals. A paternity correlation of 14% suggests
that there is a low probability of finding full sibs in the progeny. Cross-amplification of the 26 microsatellite loci available
for C. pubescens was evaluated across 11 different Centrosema species. Nineteen of the 26 tested microsatellites were successfully transferable across the Centrosema species. The polymorphism information content and discriminating power evaluated had averages of 0.64 and 0.77, respectively.
A total of three clusters were assembled to demonstrate the genetic relationships between Centrosema species. The transferable microsatellite loci should be useful for exploiting the genetic resources of the Centrosema species and determining the outcrossing rate, which are essential for proposing effective approaches for conservation and
for establishing strategies for the selection and improvement of Centrosema spp.
KeywordsTropical legume–Cross-amplification–Mating system–Autogamy
Euphytica 04/2012; 181(2):223-235. · 1.55 Impact Factor
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ABSTRACT: Meliponaseminigramerrillae is one of the main pollinators of Central Amazon native flora. These stingless bees are reared as economic alternative and
for forest conservation purposes. Nine polymorphic microsatellite markers were characterized in 44 genotypes of M.seminigramerrillae from the Meliponary of the Instituto Nacional de Pesquisas da Amazonia, INPA (3° 05.838′S 059° 59.103′W), Manaus, Brazil.
The number of alleles observed for each locus ranged from 3 to 8, with an average of 3.7 alleles per locus. The polymorphism
information content (PIC) varied between 0.21 and 0.81 (average 0.50) and the discriminating power (D) ranged from 0.59 to 0.98 (average 0.77). The observed heterozygosity (H
o) and the expected heterozygosity (H
e) were 0.04–0.68 and 0.19–0.84, respectively. A cross-amplification test in three Melipona species suggested potential transferability of these microsatellites. These microsatellite markers should be useful for characterizing
natural populations and specie conservation.
Conservation Genetics Resources 04/2012; 1(1):487-490. · 0.49 Impact Factor
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ABSTRACT: The silverleaf whitefly (Bemisia tabaci) is actually a complex of morphologically indistinct species that vary in their capacity to transmit plant viruses and to induce physiological disorders in plants of economic importance. The worldwide impact of this whitefly has increased greatly, as it is a vector of various types of phytovirus, especially geminiviruses, in plants of economic importance. The adaptability of B. tabaci to many regions of the world has fostered the appearance of various biotypes that attack a broad spectrum of host plants. We developed microsatellite markers to study genetic variability and population structure of this whitefly in Brazil. Thirteen polymorphic microsatellite markers were isolated and characterized in 20 individuals from a natural population that were collected in soybean in Campinas (SP). The number of alleles per locus ranged from one to two, and the expected heterozygosity ranged from 0.000 to 0.505. These microsatellite markers will be useful for studies and management of B. tabaci. The low polymorphism found in these molecular markers is probably associated with homology of genes expressed in these markers.
Genetics and molecular research: GMR 01/2012; 11(4):3899-903. · 1.18 Impact Factor
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ABSTRACT: The botanical classification of Stylosanthes guianensis is controversial, and few studies have used molecular markers to analyse this species. We used microsatellite markers to study the genetic diversity and population structure of S. guianensis and compare our results with the current infraspecific botanical classification.
A representative sample from the S. guianensis Brazilian germplasm collection (150 accessions) was analysed using 20 microsatellite loci. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign accessions into clusters. A dendrogram was constructed based on Roger's genetic distances.
The number of alleles per locus varied from 2 to 11, with an average of 4.7. The observed (H(O)) and expected (H(E)) heterozygosity values varied from 0 to 0.58 (mean of 0.18) and from 0.04 to 0.83 (mean of 0.55), respectively. Nine groups were assembled in STRUCTURE, and these groups were consistent with clusters inferred from the genetic distances and taxonomic varieties described for S. guianensis. The G(ST) among the nine groups was 0.46.
The low H(O) and the G(ST) values observed are in agreement with the outcrossing rate (26 %) estimated for this species. The data indicate a high genetic diversity among and within the botanical varieties and suggest that microsatellite-based information can be combined with classical taxonomy to elucidate infraspecific levels.
AoB plants. 01/2012; 2012:pls001.
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Ramesh K Aggarwal,
Joel Allainguillaume,
M M Bajay,
Santan Barthwal,
P Bertolino,
Priti Chauhan,
Sofia Consuegra,
Adam Croxford,
Desiré L Dalton,
E den Belder, [......],
Koji Takayama,
Mariko Tamura,
Yoichi Tateishi,
Delphine Vanhaecke,
Ninh V Vu,
R Wickneswari,
A S Williams,
G M Wimp,
Volker Witte, M I Zucchi
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ABSTRACT: This article documents the addition of 229 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Acacia auriculiformis × Acacia mangium hybrid, Alabama argillacea, Anoplopoma fimbria, Aplochiton zebra, Brevicoryne brassicae, Bruguiera gymnorhiza, Bucorvus leadbeateri, Delphacodes detecta, Tumidagena minuta, Dictyostelium giganteum, Echinogammarus berilloni, Epimedium sagittatum, Fraxinus excelsior, Labeo chrysophekadion, Oncorhynchus clarki lewisi, Paratrechina longicornis, Phaeocystis antarctica, Pinus roxburghii and Potamilus capax. These loci were cross-tested on the following species: Acacia peregrinalis, Acacia crassicarpa, Bruguiera cylindrica, Delphacodes detecta, Tumidagena minuta, Dictyostelium macrocephalum, Dictyostelium discoideum, Dictyostelium purpureum, Dictyostelium mucoroides, Dictyostelium rosarium, Polysphondylium pallidum, Epimedium brevicornum, Epimedium koreanum, Epimedium pubescens, Epimedium wushanese and Fraxinus angustifolia.
Molecular Ecology Resources 01/2011; 11(1):219-22. · 3.06 Impact Factor
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ABSTRACT: Bemisia tabaci (Hemiptera: Aleyrodidae) consists of a complex of morphologically indistinct biotypes that vary mainly in their capacity to transmit plant viruses and to induce physiological disorders in plants of economic importance. The adaptability of B. tabaci to many regions of the world has fostered the appearance of various biotypes and has resulted in a broad spectrum of host plants. Our goal was to identify which biotypes were present in four B. tabaci populations in Brazil. We quantified genetic variability between and within populations. Three individuals were collected from three host plant species: two populations on soybean (Campinas and Rondonópolis), one on pumpkin (Barreiras) and one on tomato (Cruz das Almas) in three States of Brazil (São Paulo, Mato Grosso, and Bahia). We chose one sequence of the B biotype, obtained from GenBank; the Campinas population, which had been previously characterized as biotype B, was used as a control for this biotype. We also included one sequence of the Q biotype, obtained from GenBank, as an outgroup. The COI region of the mtDNA gene was partially amplified with the CI-J-2195 and L2-N-3014 pair of primers, and the reaction products were sequenced. Based on distance-based algorithm analyses, we found that all haplotypes belong to biotype B, which was confirmed by the haplotype network. Genetic structure analyses showed that the host plant species does not influence population structuring of this pest; only the geographic location mattered.
Genetics and molecular research: GMR 01/2011; 10(3):2155-64. · 1.18 Impact Factor
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ABSTRACT: Various species of the Physalaemus cuvieri group of frogs are difficult to distinguish morphologically, making molecular analysis an attractive alternative for indentifying members of this group, which is considered to be at risk because of loss of habitat. The genetic structure of natural populations of P. ephippifer and P. albonotatus species was investigated and analyzed, together with that of five previously studied populations of P. cuvieri. Nine microsatellite loci were used in the analyses. The overall G(ST) value (0.46) revealed high genetic variation among the populations, as expected for different species. Bayesian analysis implemented by the STRUCTURE software clustered the seven populations into seven groups (K = 7). All the P. albonotatus and P. ephippifer specimens were grouped into a single cluster, both species showing clear differentiation from P. cuvieri. The different grouping based on these microsatellites of some P. cuvieri individuals from Porto Nacional and from Passo Fundo suggests that they could be a new species, indicating a necessity for taxonomic reevaluation. Despite the intrinsic difficulties in analyzing closely related species, the nine microsatellite loci were found to be adequate for distinguishing these three species of the P. cuvieri group and their populations.
Genetics and molecular research: GMR 01/2011; 10(3):1434-44. · 1.18 Impact Factor
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ABSTRACT: Turmeric (Curcuma longa) is a triploid, vegetatively propagated crop introduced early during the colonization of Brazil. Turmeric rhizomes are ground into a powder used as a natural dye in the food industry, although recent research suggests a greater potential for the development of drugs and cosmetics. In Brazil, little is known about the genetic variability available for crop improvement. We examined the genetic diversity among turmeric accessions from a Brazilian germplasm collection comprising 39 accessions collected from the States of Goiás, Mato Grosso do Sul, Minas Gerais, São Paulo, and Pará. For comparison, 18 additional genotypes were analyzed, including samples from India and Puerto Rico. Total DNA was extracted from lyophilized leaf tissue and genetic analysis was performed using 17 microsatellite markers (single-sequence repeats). Shannon-Weiner indexes ranged from 0.017 (Minas Gerais) to 0.316 (São Paulo). Analyses of molecular variance (AMOVA) demonstrated major differences between countries (63.4%) and that most of the genetic diversity in Brazil is found within states (75.3%). Genotypes from São Paulo State were the most divergent and potentially useful for crop improvement. Structure analysis indicated two main groups of accessions. These results can help target future collecting efforts for introduction of new materials needed to develop more productive and better adapted cultivars.
Genetics and molecular research: GMR 01/2011; 10(1):419-28. · 1.18 Impact Factor
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ABSTRACT: • Premise of the study: Paspalum atratum is a perennial, cespitose, tropical grass native to Central and South America. This species belongs to a polyploid complex (Plicatula group) little known at the genetic level. The characterized microsatellite markers provide new informative tools for further studies of the hybridization, mating systems, and structure of the population. • Methods and Results: Using the microsatellite-enriched library method, we isolated and characterized 19 microsatellite markers from P. atratum. Eleven of them were polymorphic, showing a variable degree of variation, while eight were monomorphic in the samples analyzed. Additionally, the transferability of these microsatellite markers was tested in other species. • Conclusions: These results suggest that the characterized markers have enough discriminatory potential to be used in genetic characterizations of Paspalum taxa, which are based on an understanding of their mating systems and genetic structure, as well as in understanding the evolutionary processes involved in the evolution of groups of Paspalum.
American Journal of Botany 11/2010; 97(11):e107-10. · 2.66 Impact Factor
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ABSTRACT: Brachiaria humidicola (Rendle) Schweick. is a warm-season grass commonly used as forage in the tropics. Accessions of this species were collected in eastern Africa and massively introduced into South America in the 1980s. Several of these accessions form a germplasm collection at the Brazilian Agricultural Research Corporation. However, apomixis, ploidy, and limited knowledge of the genetic basis of this germplasm collection have constrained breeding activities. The objectives of this work were to identify genetic variability in the Brazilian B. humidicola germplasm collection using microsatellite markers and to compare the results with information on the following: (1) collection sites of the accessions; (2) reproductive mode and ploidy levels; and (3) genetic diversity revealed by morphological traits. The evaluated germplasm population is highly structured into four major groups. The sole sexual accession did not group with any of the clusters. Genetic dissimilarities did not correlate with either geographic distances or genetic distances inferred from morphological descriptors. Additionally, the genetic structure identified in this collection did not correspond to differences in ploidy level. Alleles exclusive to either sexual or apomictic accessions were identified, suggesting that further evaluation of the association of these loci with apospory should be carried out.
Genome 09/2010; 53(9):698-709. · 1.65 Impact Factor
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Cynthia M Anderson,
Gallego J Aparicio,
Alain R Atangana,
Jean Beaulieu,
M W Bruford,
Forrest Cain,
T Campos,
A Cariani,
M A Carvalho,
Nan Chen, [......],
Huan L Wang,
LE Wang,
Rémi A Wattier,
Maren Wellenreuther,
Cong X Xie,
L Zane,
Xiu J Zhang,
Yong Zhang,
Zhimeng Zhuang, M I Zucchi
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ABSTRACT: This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mülleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.
Molecular Ecology Resources 05/2010; 10(3):576-579. · 3.06 Impact Factor
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ABSTRACT: With 3 tablesAbstractYellow and sweet passion fruit are insect-pollinated species native to the tropics. Fruits are used commercially for human consumption worldwide. The yellow passion fruit is an outcrossing species with self-incompatible flowers. However, the reproductive system of the sweet passion fruit (Passiflora alata) has not been well elucidated. The objective of this work was to characterize aspects of the mating system in the sweet passion fruit using random amplified polymorphic DNA (RAPD) and microsatellite markers, particularly the rate of outcrossing in P. alata progenies. A multilocus outcrossing rate of tm = 0.994 was determined from RAPD and tm = 0.940 from microsatellites, supporting P. alata as an outcrossing species. The fixation indices of the maternal generation (Fm) were −0.200 and 0.071 with RAPD and microsatellite loci, respectively, indicating the absence of inbreeding in the maternal generation. The paternity correlation (rp) varied from −0.008 with RAPD markers to 0.208 with microsatellite markers, suggesting a low probability of finding full sibs within the progenies. The results demonstrated that all progenies assessed in this study were derived from outcrossing.
Plant Breeding 02/2010; 129(6):727 - 730. · 1.60 Impact Factor
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C. M. Anderson,
G. J. Aparicio,
A. R. Atangana,
J. Beaulieu,
M. W. Bruford,
F. Cain,
T. Campos,
A. Cariani,
M. A. Carvalho,
N. Chen, [......],
L. Wang,
R. A. Wattier,
M. Wellenreuther,
C. X. Xie,
L. Zane,
X. J. Zhang,
Y. Zhang,
Z. M. Zhuang, M. I. Zucchi,
Dev Mol Ecology Resources Primer
[show abstract]
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ABSTRACT: This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mulleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.
Molecular Ecology Resources. 01/2010; 10(3):576-579.
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ABSTRACT: With 1 figure and 1 tableAbstractCurcuma longa L. is a sterile, triploid, vegetatively-propagated crop cultivated mainly in Southeast Asia. When dried rhizomes are ground, the resulting yellow powder is used by the food industry as a natural food dye. Moreover, many pharmacological compounds have broadened the commercial application of the crop. However, conventional breeding is difficult and hence, improvement has been limited to germplasm selection. To better utilize the germplasm collections and facilitate genotype selection, a total of 17 polymorphic microsatellite loci were developed using a CT/GT/CTT enriched genomic library. All microsatellites resulted in amplified PCR products, showing a banding pattern of 2–11 polymorphic bands per locus, enabling genotype discrimination. These results can be used in further studies aimed at characterizing C. longa genetic resource collections and also to improve breeding strategies.
Plant Breeding 11/2009; 129(5):570 - 573. · 1.60 Impact Factor
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ABSTRACT: Lychnophora pinaster Mart. (Asteraceae) is a Brazilian medicinal plant, extensively employed in popular medicine as an anti-inflammatory, analgesic and healing agent. Thirteen polymorphic microsatellite markers were developed and optimized for L. pinaster from an enriched genomic library. The markers were used to analyse 37 plants from two native populations, generating an average number of 6.6 alleles per polymorphic locus. These loci are important tools for future studies of population genetics.
Molecular Ecology Resources 05/2009; 9(3):811-4. · 3.06 Impact Factor
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Conservation Genetics Research. 04/2009;
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Conservation Genetics. 02/2009; online first:DOI 10.1007/s10592-009-9872-6.
