Sara Volk

Johns Hopkins University, Baltimore, Maryland, United States

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Publications (3)13.39 Total impact


    Comparative Biochemistry and Physiology - Part A Molecular & Integrative Physiology 07/2008; 150(3). DOI:10.1016/j.cbpa.2008.04.470 · 1.97 Impact Factor
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    ABSTRACT: Lys-63-linked multiubiquitin chains play important roles in signal transduction in yeast and in mammals, but the functions for this type of chain in plants remain to be defined. The RING domain protein RGLG2 (for RING domain Ligase2) from Arabidopsis thaliana can be N-terminally myristoylated and localizes to the plasma membrane. It can form Lys-63-linked multiubiquitin chains in an in vitro reaction. RGLG2 has overlapping functions with its closest sequelog, RGLG1, and single mutants in either gene are inconspicuous. rglg1 rglg2 double mutant plants exhibit loss of apical dominance and altered phyllotaxy, two traits critically influenced by the plant hormone auxin. Auxin and cytokinin levels are changed, and the plants show a decreased response to exogenously added auxin. Changes in the abundance of PIN family auxin transport proteins and synthetic lethality with a mutation in the auxin transport regulator BIG suggest that the directional flow of auxin is modulated by RGLG activity. Modification of proteins by Lys-63-linked multiubiquitin chains is thus important for hormone-regulated, basic plant architecture.
    The Plant Cell 07/2007; 19(6):1898-911. DOI:10.1105/tpc.107.052035 · 9.34 Impact Factor
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    Sara Volk · Min Wang · Cecile M Pickart ·
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    ABSTRACT: Ubiquitin can be conjugated to lysine residues of other ubiquitin molecules to form polymers called polyubiquitin chains. Ubiquitin has seven lysine residues, creating the potential for seven distinct types of chains, at least five of which have been observed in vitro or in vivo. A subset of these chains mediates substrate targeting to proteasomes, whereas other types of chains have been implicated in nonproteolytic signaling pathways. In this chapter, we outline chemical and genetic strategies that can be used to deduce (or control) the structures of polyubiquitin chains in vitro and in living cells.
    Methods in Enzymology 02/2005; 399:3-20. DOI:10.1016/S0076-6879(05)99001-0 · 2.09 Impact Factor