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ABSTRACT: Si-Miao-Yong-An (Trade name: Mai-Luo-Ning), a Chinese herbal formulation comprising Flos Lonicerae Japonicae, Radix Scrophulariae Ningpoensis, Radix Angelicae Sinensis and Radix Glycyrrhizae Uralensis, has been used in treating ischemic cardiovascular and cerebrovascular diseases for many years. Clinical and experimental studies have shown that Si-Miao-Yong-An can inhibit the inflammatory response and antagonize the blood clotting process.
To investigate the effect of Si-Miao-Yong-An on atherosclerotic plaque stability in rabbit model.
Seventy male rabbits were divided into four groups. Rabbits in the normal group were fed with normal diet, while rabbits in model group and drug treatment groups were fed with high cholesterol diet, underwent BSA-induced immunologic injury and balloon-induced mechanical injury. After atherosclerotic rabbits were treated with simvastatin or Si-Miao-Yong-An for 16 weeks, blood and aorta in four groups were collected for analysis.
Si-Miao-Yong-An reduced the level of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) in blood after treatment for 16 weeks. Compared with model group, Si-Miao-Yong-An decreased the content of many inflammatory cytokines in blood and plaque. Morphological analysis of abdominal aorta showed that Si-Miao-Yong-An increased fibrous cap thickness and smooth muscle cells, reduced lipid core area and macrophages, and contributed to inhibit matrix degradation and inflammatory response.
In this study, we provided evidence for that Si-Miao-Yong-An could promote the stability of atherosclerotic plaque in the rabbit model, indicating that this medicine was a reasonable drug treating cardiovascular diseases in clinical.
Journal of ethnopharmacology 06/2012; 143(1):241-8. · 2.32 Impact Factor
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ABSTRACT: To investigate the effects of ferulic acid on the migration of vascular smooth muscle cells (VSMCs) and its correlated mechanisms.
VSMCs were in vitro cultured. Under the vascular endothelial growth factor (VEGF) induced conditions, the VSMCs migration were detected using the scratch test and the invasion assay after intervened by ferulic acid. The effects on the mRNA expressions of matrix metalloproteinase, (MMP)-2 and MMP-9 were detected using RT-PCR. The effects on the protein expressions of the tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2 were detected using Western blot.
(1) Ferulic acid (10(2) ng/mL and 10(3) ng/mL) could inhibit VEGF-induced VSMCs migration. (2) Ferulic acid (10(2) ng/mL and 10(3) ng/mL) could down-regulate the VEGF-induced VSMCs migration by inhibiting MMP-9 mRNA expression. (3) Ferulic acid (10(2) ng/mL and 10(3) ng/mL) up-regulated VEGF-induced VSMCs TIMP-2 protein expressions.
Ferulic acid (10(2) ng/mL and 10(3) ng/mL) could inhibit VEGF-induced VSMCs migration by inhibiting the MMP-9 mRNA expression, and increasing the TIMP-2 protein expression.
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 02/2012; 32(2):229-33.
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ABSTRACT: Along with the extensive application of intervention in treating coronary heart disease (CHD), problems such as post-interventional restenosis, no reflowing, and slow flowing, and so on increasingly emerge. Chinese medicine and pharmacy occupy advantages covering preventing post-intervention risk factors, improving and relieving patients' symptoms, and so on. It is a good support and supplement for routine therapies in modern medicine. Taking the pathological stages of CHD and clinical problems of coronary intervention as the breakthrough points, the Chinese medical syndromes and correspondent Chinese medicine recipes and herbs were preliminarily studied by combining Chinese medicine's recognition and clinical observation on coronary intervention.
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 07/2011; 31(7):985-7.
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ABSTRACT: Based on the combination of the traditional TCM theory and the idea and results of modern medical research on atherosclerosis (AS), the authors understand the pathology of AS in three stages and have studied the stage-oriented differential treatment of AS, namely, 1) the formation stage of AS plaque treated with the principle of regulating the liver, nourishing the kidneys and strengthening the spleen; 2) the stable stage of AS plaque treated with the principle of clearing away heat and toxic materials; and 3) the breaking stage of AS plaque treated with the principle of promoting blood circulation and removing blood stasis. We propose that the TCM treatment of AS should be based on the achievements of modern medical research, utilizing stage-oriented treatment according to the different stages of the formation and development of AS plaque, so as to improve the therapeutic effects.
Journal of Traditional Chinese Medicine 09/2009; 29(3):201-4. · 0.30 Impact Factor
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ABSTRACT: To observe the intervening effect and acting mechanism of Bushen Kangshuai Tablet (BKT) on rabbits' atherosclerosis (AS).
Thirty-six white Japanese rabbits were randomly divided into four groups: 6 in the normal control group, and each 10 in the model group, the BKT group and the simvastatin group. The AS model was established by high fatty diet feeding from the 1st to the 10th week, combined with immune injury at the 2nd week and femoral arterial balloon tearing at the 4th week. The medication of BKT and simvastatin was given during the whole 10-week course. Levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), interleukin-1 (IL-1), monocyte chemotactic protein-1 (MCP-1), and tumor necrosis factor-alpha (TNF-alpha) were measured at the beginning and the 3rd, 6th and 10th weekend of the experiment. The nuclear factor-kappa B (NF-kappaB) expression in the aortic wall, ratio of plaque area to intima area (PA/IA), intima thickness (IT), aortic intima/media thickness ratio (IT/MT) and intima hyperplasia index (IHI) were measured at the terminal of the experiment. The correlation analysis was conducted between serum lipids, inflammation factors and IHI.
Compared with the normal control group, all indices of the blood lipids and Inflammation factors measured at various time points, and the PA/IA, IT/MT ratios as well as IHI in the model group were higher (P<0.01, P<0.05). Compared with the model group, the levels of IL-1, MCP-1, TNF-alpha, NF-kappaB as well as the ratios of PA/IA, IT/MT and IHI were lower in the two treated groups at all time points after treatment (P<0.01 or P<0.05). Pearson correlation analysis showed positive correlation between TC with IL-1, and TNF-alpha with IHI in the model group (P< 0.05); also between TC with IHI, IL-1 with IHI, and TNF-alpha with IHI and IL-1 in the BKT group (P<0.05), while no correlation between blood lipids with inflammation factors was observed.
BKT could suppress the inflammation reaction in rabbits to prevent AS formation, the action is not directly correlated with the blood lipid level.
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 09/2009; 29(9):816-20.
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ABSTRACT: Aspirin (acetylsalicylic acid) is a nonsteroidal anti-inflammatory drug. Despite its wide uses for more than 100 years, knowledge about mechanism of action and therapeutic issues of aspirin are still under discussion. The use of aspirin has been changed from an analgesic, anti-pyretic and anti-inflammatory agent to an anti-thrombotic agent, especially in secondary prevention of cardiovascular events. Aspirin has reduced the risk of cardiovascular events by 25%. However, the phenomenon of "aspirin resistance" has been described that in 5%-60% of patients aspirin may not achieve adequate efficacy of suppressing platelet activity. The convinced causes of this phenomenon are still unknown. It is probably due to drugs interaction, inadequate dosage and so on. By far the existing studies of aspirin are insufficient to explain all phenomena of aspirin resistance. And the results are not always uniform about the same research. Therefore, the characteristics in different population with aspirin resistance may account for the complexity. It is unrealistic to elucidate all aspirin resistance by only one pathway. More studies are required to investigate the mechanisms in different population respectively. According to the theory of traditional Chinese medicine and the trait of cardiovascular disease, which often relapses and has a long history, aspirin resistance should be considered as collaterals disease. It can be treated with aspirin and traditional Chinese drugs which have the power to strengthen body resistance, reduce phlegm, remove blood stasis and toxic materials from meridians. The problem of aspirin resistance might be solved by this way, because the traditional Chinese medicine has the superiority of selecting appropriate therapeutic methods based on syndrome differentiation for different population and regulating the whole body's function. Subsequently, cardiovascular disease might be effectively prevented.
Journal of Chinese Integrative Medicine 06/2007; 5(3):259-62.
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ABSTRACT: Clinical effect is of great importance to traditional Chinese medicine (TCM), and the rigorous and scientific methods for clinical effect assessment should be highly stressed in the clinical research of TCM. In this article, the authors analyzed the current status of the effect assessment of TCM and proposed some measures to improve the current effect assessment of TCM, in the hope of establishing an internationally accepted system of effect assessment reflecting the superiority and characteristics of TCM therapies.
Journal of Chinese Integrative Medicine 06/2005; 3(3):181-3.
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ABSTRACT: Single nucleotide polymorphism (SNP) is the third generation genetic marker. SNP detection now is becoming increasingly important means in molecular diagnostics, clinical assay and novel drug development. It plays an essential role in drug resistance and anaphylactic reaction and has the importance in theoretical studies of gene location, hereditary diseases and human origin. With the accomplishment of human genome sequencing, the genotyping and discovering of SNP are becoming hot subjects in genetics and biomedicine researches. The methods for SNP detection were renewed rapidly and developed fast in past few years. In this review, several newly established detection methods including gene chip, molecular probe, fluorescence polarization and resonance, mass spectrometry, and bacterial magnetic particle are discussed. It could be expected that an accurate and sensitive, simple and easy-to-handle SNP technology with low cost and high throughput will be available on the basis of research breakthroughs of biochemistry, engineering and analytic software.
Hereditas (Beijing) 02/2005; 27(1):123-9.
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Jun-Ping ZHANG
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ABSTRACT: Clinical effect is of great importance to traditional Chinese medicine (TCM), and the rigorous and scientific methods for clinical effect assessment should be highly stressed in the clinical research of TCM. In this article, the authors analyzed the current status of the effect assessment of TCM and proposed some measures to improve the current effect assessment of TCM, in the hope of establishing an internationally accepted system of effect assessment reflecting the superiority and characteristics of TCM therapies.
Zhong Xi Yi Jie He Xue Bao. 01/2005;
