-
[show abstract]
[hide abstract]
ABSTRACT: Nowadays methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common multidrug resistant bacteria both in hospitals and in the community. In the last two decades, there has been growing concern about the increasing resistance to MRSA of the most potent antibiotic glycopeptides. MRSA infection poses a serious problem for physicians and their patients. Photosensitizer-mediated antimicrobial photodynamic therapy (PDT) appears to be a promising and innovative approach for treating multidrug resistant infection. In spite of encouraging reports of the use of antimicrobial PDT to inactivate MRSA in large in vitro studies, there are only few in vivo studies. Therefore, applying PDT in the clinic for MRSA infection is still a long way off.
BioMed research international. 01/2013; 2013:159157.
-
[show abstract]
[hide abstract]
ABSTRACT: This paper reviews the new progress in the research of fractional carbon dioxide laser in treating hypertrophic scar after burn injury, which remains a challenging problem for burn care surgeons. There have been many traditional therapeutic approaches, such as compression remedy, operation, and so on. However, a satisfactory method is lacking to date. In recent years, the newly developed fractional carbon dioxide laser has been employed to treat different kinds of scars, and it has been proved to be effective in terms of an improvement of scar color, texture, and rigidity. It seems to be a promising method for scar treatment in future.
Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns 12/2012; 28(6):465-467.
-
[show abstract]
[hide abstract]
ABSTRACT: Low level laser therapy (LLLT) is a therapeutic method which regulates the biological behavior of cells with light. The effects of LLLT consist of promotion of tissue repair, inhibition of inflammation, and relief of pain by promoting or inhibiting the cell proliferation, increasing or decreasing the release of some bioactive substances. Therefore, LLLT is also known as photomodulation. At present, there are many relevant experimental studies of LLLT abroad, and they are also used clinically. This article reviews the effect of LLLT on wound healing.
Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns 12/2012; 28(6):462-464.
-
[show abstract]
[hide abstract]
ABSTRACT: This article reviews the advance in the research of both the mechanism of photodynamic antimicrobial chemotherapy (PACT) based on the principle of photodynamic therapy and the application of PACT in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infection. Nowadays an inexorable prevalence of resistant bacteria observed worldwide, among which MRSA strains typically have a high potential of provoking outbreaks with intractability, makes it a pressing issue to develop new germicidal strategies. PACT is an emerging photochemistry-based technology. In the presence of oxygen, PACT, with the aid of the activation of photosensitizers to generate reactive oxygen species by the wavelength-specific light, destroys the structure of bacteria selectively and efficiently, with much lower bacterial resistance level than that of antibiotics. We expect that further research would elucidate the mechanism and develop clinical applications of PACT, and it may become a novel approach to solve the problem of MRSA infection in the future.
Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns 10/2012; 28(5):367-9.
-
[show abstract]
[hide abstract]
ABSTRACT: Acute systemic inflammatory response to severe skin burn injury mediates burn-induced acute lung injury. Ulinastatin is potentially an effective intervention, because it attenuates the systemic inflammatory response induced by endotoxin and improves myocardial function during ischemic shock and reperfusion.
Rats received full-thickness burn wounds to 30% total body surface area followed by delayed resuscitation. The treatment group received 50,000 U/kg of ulinastatin and the burn group was given vehicle only. A sham group was not burned but otherwise was treated identically. After killing, blood and lung samples were harvested for histology and measurement of inflammatory mediators.
Administration of ulinastatin significantly decreased the mRNA and protein levels of tumor necrosis factor-alpha, interleukin-1β, -6, and -8 both locally and systemically in burn-injured rats. The secretion of neutrophil elastase and myeloperoxidase in the lung and the expression of intercellular adhesion molecule-1 on the surface of lung epithelium were inhibited by ulinastatin. Ulinastatin also reduced the increase in pulmonary microvascular permeability. Consistent with these findings, ulinastatin ameliorated the lung edema and pulmonary oxygenation in burn-injured rats.
These results indicate that the inhibitory effects of ulinastatin on inflammatory mediator production, neutrophil activation, and microvascular permeability are associated with the recovery of pulmonary functions in severe burn-induced acute lung injury and suggest that ulinastatin may serve as a potential therapeutic administration in critical burn care.
The Journal of trauma 09/2011; 71(5):1297-304. · 2.48 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the effectiveness and mechanism of recombinant human granulocyte-macrophage colony-stimulating factor (rhGMCSF) gel on wound debridement and healing of deep II thickness burn.
Between December 2008 and December 2010, 58 patients with deep II thickness burn, accorded with the inclusive criteria, were collected. There were 36 males and 22 females with an average age of 32.4 years (range, 12-67 years). The causes were hot liquid in 38 cases and fire in 20 cases. The time from injury to treatment was 1-3 days (mean, 2.1 days). In this randomized, double-blind, and self-control study, all patients were randomly divided into 2 groups, wounds were treated with rhGMCSF gel (test group) or gel matrix (control group). There was no significant difference in wound area between 2 groups (P > 0.05). The time of completed removal eschar and the percentage of removal-area of eschar were recorded at 2, 6, 10, 14, and 18 days during healing process. The time of wound healing was also recorded.
Compared with control group, the necrotic tissues on the burn wound got soft in test group at 4 days after treatment. At 6 days, they loosened and eventually sloughed off. The wound bed presented in red and white, followed by rapidly growing granulation tissues. Except 18 days after treatment, there were significant differences in the percentage of removal-area of eschar between 2 groups (P < 0.05). The time of completed removal eschar in test group [(7.71 +/- 2.76) days] was significantly shorter than that in control group [(14.71 +/- 3.63) days] (t = 13.726, P = 0.000). The time of wound healing in test group was (18.41 +/- 2.47) days, which was significantly shorter than that in control group [(23.58 +/- 3.35) days] (t = 15.763, P = 0.000).
Compared with the gel matrix, the rhGMCSF gel may promote wound debridement and healing in deep II thickness burn.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 09/2011; 25(9):1059-62.
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the feasibility of photochemical tissue bonding (PTB) technique in repairing limbal stem cell (LSC) deficiency and the effect on cornea wound healing.
LSCs were isolated from limbus of New Zealand rabbits by tissue block culture method, and then the LSCs of 2nd passage were cultured on de-epithelialized human amniotic membrane (HAM) for 3 weeks to prepare the HAM/LSC grafts. The LSC deficiency models of the left eyes were established by 0.5 mol/L NaOH in 24 New Zealand female rabbits, aged 3-4 months and weighing 1.5-2.0 kg. HAM/LSC grafts were used to repair the cornea wounds by sutures (suture group, n = 12) or by PTB technique (PTB group, n = 12). The gross was observed including the corneal transparency, erythema, and new blood vessel formation after surgery. At 3 and 28 days, the inflammatory cytokine of interleukin 1beta (IL-1beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) were assayed by ELISA method; and the amount of new blood vessels were quantified by immunohistochemistry staining at 28 days.
All animals survived to the end of the experiment. At 3 days, there was no obvious difference in the corneal transparency between 2 groups; at 28 days, the corneal transparency of PTB group was higher than that of suture group, and new blood vessels decreased. HE staining showed that mass inflammatory cells infiltrated between graft and cornea basal layer at 3 days, and no new blood vessel formed. inflammatory cells infiltration significantly decreased at 28 days in PTB group; the amount of new blood vessels was (2.0 +/- 0.8)/HP in PTB group and was (6.3 +/- 1.3)/HP in suture group, showing significant difference (t = 7.966, P = 0.002). At 28 days, the concentrations of inflammatory cytokine of IL-1beta, IL-6, and TNF-alpha in suture group were significantly higher than those in PTB group (P < 0.05); however, no significant differences were observed between 2 groups at 3 days (P > 0.05).
PTB technique can be used to fix HAM/LSC grafts, which can decrease inflammatory cell infiltration and new vessel formation, and improve the outcomes when compared with suture technique.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery 09/2011; 25(9):1110-4.
-
[show abstract]
[hide abstract]
ABSTRACT: Hydrogen has been reported to selectively quench detrimental reactive oxygen species, particularly hydroxyl radical, and to prevent myocardial or hepatic ischemia/reperfusion injury in multiple models. The aim of this study is to investigate whether hydrogen protects against severe burn-induced acute lung injury in rats. Rats were divided into four groups: sham plus normal saline, burn injury plus normal saline, burn injury plus hydrogen-rich saline, and burn injury plus edaravone. Animals were given full-thickness burn wounds (30% TBSA) using boiling water, except the sham group that was treated with room temperature water. The rats in hydrogen group received 5 ml/kg of hydrogen-rich saline, sham and burn controls obtained the same amount of saline, and the edaravone group was treated with 9 mg/kg of edaravone in saline. Lactated Ringer's solution was given at 6 hours postburn. The lungs were harvested 12 hours postburn for laboratory investigations. Severe burns with delayed resuscitation rapidly caused lung edema and impaired oxygenation in rats. These dysfunctions were ameliorated by administration of hydrogen-rich saline or edaravone. When compared with the burn injury plus normal saline group, hydrogen-rich saline or edaravone group significantly attenuated the pulmonary oxidative products, such as malondialdehyde, carbonyl, and 8-hydroxy-2'-deoxyguanosine. Furthermore, administration of hydrogen-rich saline or edaravone dramatically reduced the pulmonary levels of pulmonary inflammation mediators and myeloperoxidase. Intraperitoneal administration of hydrogen-rich saline improves pulmonary function by reducing oxidative stress and inflammatory response in severe burn-induced acute lung injury.
Journal of burn care & research: official publication of the American Burn Association 03/2011; 32(3):e82-91. · 1.37 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: To investigate the effect of lipid protein complex (LPC) from burn wound on the pulmonary vascular permeability.
Fifty-two SD rats were made into 30%III degrees TBSA burn models. Six milliliters of peripheral blood were extracted from each rat 3 days after and LPC was isolated. Then 32 rats were killed. Their lungs and hearts were isolated to be made into experimental model of isolated perfused heart and lung and divided into 4 experimental groups perfused with different kinds of perfusion fluid containing LPC, serum from rats with burn, serum from normal rats, or perfusion fluid without above mentioned components (control group). Five minutes and 35 minutes after the perfusion, the lung weigh gain (LWG), fluid filtration coefficient (Kf) and pulmonary albumin permeability-surface area product (PS) were detected.
Five minutes after perfusion the LWG was 0.08 +/- 0.04 g in the burn serum group, and 0.07 +/- 0.04 g in the LPS group, both significantly higher than that in the control group (0.03 +/- 0.03, both P < 0.01). Thirty-five minutes after, the LWG was 0.56 +/- 0.12 g in the burn serum group, and 0.40 +/- 0.10 g in the LPS group, both significantly higher than that in the control group (0.26 +/- 0.08 g, P < 0.01) and with a significant difference between the burn serum group and LPC group (P < 0.05). PS was 7.6 +/- 1.3 x 10(-2) cm(3)/min x g DLW in the burn serum group, significantly higher than that in the control group (4.2 +/- 1.1 x 10(-2) cm(3)/min x g DLW, P < 0.01), and LPC group (4.6 +/- 1.2 2 x 10(-2) cm(3)/min x g DLW, P - 0.01), without a significant difference between the LPC and control group. Kf was 1.05 +/- 0.20 ml/min x kPa x g DLW in the burn serum group, and 0.89 +/- 0.13 ml/min x kPa x g DLW in the LPC group, both significantly higher than that in control group (0.32 +/- 0.09 ml/min x kPa x g DLW, P < 0.01) and with a significant difference between the burn serum group and LPC group (P < 0.05). No difference in LWG, PS, and Kf was found between normal serum group and control group.
LPC from burn serum induces pneumonedema in severe burn animal and may play a role in increasing vascular permeability to small molecules.
Zhonghua yi xue za zhi 11/2002; 82(22):1561-4.
