De-Lin Zhang

Publications (6)8.61 Total impact

• Article: Serological survey of Toxoplasma gondii in Tibetan mastiffs (Canis lupus familiaris) and yaks (Bos grunniens) in Qinghai, China.

  Meng Wang, Yan-Hua Wang, Qiang Ye, Peng Meng, Hong Yin, De-Lin Zhang
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  ABSTRACT: Toxoplasma gondii is an amphixenosis which has extensive hosts. In recent years, the prevalence of T. gondii in China has been reported, while little is known on the survey of T. gondii infection in northwest China, especially in yaks (Bos grunniens) and Tibetan mastiffs (Canis lupus familiaris). The current study survey the infection of T. gondii in Tibetan mastiffs and yaks in Qinghai Province, China. The indirect hemagglutination test (IHAT) was used to examine T. gondii antibodies in 1 795 serums, including 192 Tibetan mastiffs and 1603 yaks in Qinghai Province, China. In this study, the seroprevalence of T. gondii infection was 8.52%. Twenty (10.42%) of 192 serums of Tibetan mastiffs and 133 (8.30%) of 1603 serums of yaks were seropositive. The seroprevalence of T.gondii infection in Tibetan mastiffs in breeding farm (1.08%) was lower than that in the field (19.19%), and the difference was statistically significant (P <0.05). The seroprevalence of antibodies to T.gondii in yaks ranged from 5.45% to 13.28% among the four different areas. The seroprevalence in different age groups were determined with apparent association. The results indicated that T.gondii infection was prevalent in Tibetan mastiffs and yaks, which have implications for public health in this region. To our knowledge, this is the first seroprevalence survey of Tibetan mastiffs infected by T. gondii in The People's Republic of China.
  Parasites & Vectors 02/2012; 5:35. · 2.94 Impact Factor
• Article: Seroprevalence of Toxoplasma gondii infection in Tibetan sheep in Tibet, China.

  Song-Ming Wu, Ciren Danba, Si-Yang Huang, De-Lin Zhang, Jia Chen, Ga Gong, Min-Jun Xu, Zi-Guo Yuan, Xing-Quan Zhu
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  ABSTRACT: In the present investigation, the seroprevalence of Toxoplasma gondii antibodies in 455 Tibetan sheep in Tibet, China, was examined using an indirect hemagglutination test. Of these, 26 (5.7%) Tibetan sheep were seropositive at the cut-off of 1:64 serum dilution. The seroprevalence ranged from 2.2% to 8.9% among Tibetan sheep of <1-yr-old, 1-3-yr-old, and >3-yr-old, but the differences among the age groups were not significant (P > 0.05). The prevalence in male Tibetan sheep (2.8%) was lower than that in female Tibetan sheep (6.6%), but the difference was not statistically significant (P > 0.05). The results of this survey indicated the presence of T. gondii infection in Tibetan sheep, which may cause economic losses to the local livestock industry and which poses a potential threat to human health in this area.
  Journal of Parasitology 07/2011; 97(6):1188-9. · 1.40 Impact Factor
• Article: Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens.

  Yan-Hua Wang, Xue-Rui Li, Guang-Xiang Wang, Hong Yin, Xue-Peng Cai, Bao-Quan Fu, De-Lin Zhang
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  ABSTRACT: We developed a rapid immunochromatographic strip (ICS) procedure that can detect circulating antigens in the blood of animals during the acute stage of toxoplasmosis. The aim of this study was to evaluate this test using sera from field samples and from experimentally infected animals. The sensitivity and specificity of the ICS were compared with those of an enzyme-linked immunosorbent assay (ELISA). Both assays detected circulating antigens in the sera of animals experimentally infected with the Gansu Jingtai strain of Toxoplasma gondii, and the agreement between the two assays was 100%. In the infected animals, circulating antigens could be detected as early as the second day post-infection (PI) and in all animals by the fourth day. In the 381 field serum samples, the positive rates of the ICS and ELISA were 5.2% and 5.8%, respectively. In addition, there was no cross-reactivity of the antigens with Neospora caninum. The results presented here suggest that the ICS is a feasible, convenient, rapid and effective method to detect infection by T. gondii. This test could be a powerful supplement to the current diagnostic methods. Taken together, the results of this study encourage further research toward the production of commercial diagnostic tests for detecting T. gondii in animals.
  Parasitology International 11/2010; 60(1):105-7. · 2.13 Impact Factor
• Article: Genetic characterization of Toxoplasma gondii isolates from China.

  Peng Zhou, Han Zhang, Rui-Qing Lin, De-Lin Zhang, Hui-Qun Song, Chunlei Su, Xing-Quan Zhu
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  ABSTRACT: Toxoplasma gondii infections are prevalent in humans and animals worldwide. In North America and Europe, T. gondii is highly clonal, consisting of three distinct lineages (Types I, II and III), whereas in South America, T. gondii is highly diverse with a few lineages expanded in the population. However, there is limited data on the diversity of T. gondii in Asia. Here we report the genetic characterization of T. gondii isolates from different hosts and geographical locations in China using the multilocus PCR-RFLP. A total of 17 T. gondii isolates from humans (3 strains), sheep (1 strain), pigs (5 strains) and cats (8 strains) were typed at 10 genetic markers including 9 nuclear loci SAG1, SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2 and an apicoplast locus Apico. Four genotypes were revealed, including three previously reported and one new genotype. Three isolates belong to the clonal Type I lineage, one isolate belongs to the clonal Type II lineage, and the rest 13 isolates are grouped into two genotypes. This is the first report of genetic typing of T. gondii isolates from different hosts and geographical locations in China using a number of genetic markers, which has implications for the studies of population genetic structures of T. gondii, as well as for the prevention and control of T. gondii infections in humans and animals in China.
  Parasitology International 02/2009; 58(2):193-5. · 2.13 Impact Factor
• Article: [Update on host cell invasion by Toxoplasma gondii].

  Xue-Rui Li, Yan-Hua Wang, Xiang-Zhong Zhao, De-Lin Zhang
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  ABSTRACT: This paper reviewed the importance of micronemes, dense granules, rhoptry and major surface proteins of Toxoplasma gondii, and of calcium during the host cell invasion as well as the role of T. gondii proteases in the folding and processing of these proteins.
  Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 05/2007; 25(2):154-7.
• Article: [Prevalence and drug tolerance of mycoplasma in patients with urogenital inflammation].

  Zi-He Yan, Min Zhou, Wen Zhang, De-Lin Zhang
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  ABSTRACT: To observe the prevalence and drug tolerance of mycoplasma(Ureaplasma urealyticum and Mycoplasma hominis) in patients with urogenital inflammation. Three thousand and fifty-five patients with urogenital inflammation such as non-gonococcal urethritis(NGU), chronic prostatitis or pelvic inflammation from 1999 to 2003 were included. The results of mycoplasma culture and drug sensitivity test were analyzed. A total of 992(32.5%) cases were mycoplasma positive in the 3,055 patients, and there was no significant difference in the yearly positive percentage in the 5 years (P < 0.05). Among them, 701(70.7%) were infected with Ureaplasma urealyticum, 44(4.4%) with Mycoplasma hominis, and 247(24.9%) with both Ureaplasma urealyticum and Mycoplasma hominis, the Ureaplasma urealyticum infection rate being much higher than that of Mycoplasma hominis and mixed infection (P < 0.01). The high colony counting(> or = 10(4) cfu/ml) in Ureaplasma urealyticum infection patients accounted for 76.7%, while Mycoplasma hominis infection represented only 18.2%. The results of drug tolerance test showed a higher sensitivity to doxycycline, pristinamycin, josamycin and tetracycline (94.3%, 96.6%, 86.5% and 97.4% respectively), and a lower sensitivity to erythromycin and ofloxacin (54.8% and 29.4% respectively). Ureaplasma urealyticum and Mycoplasma hominis should be detected simultaneously and the drug tolerance test is needed for the selection of appropriate antibiotics.
  Zhonghua nan ke xue = National journal of andrology 12/2003; 9(8):599-600, 603.