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ABSTRACT: BACKGROUND: Recent studies indicate that microalgal cultivation using organic carbon sources has the potential to provide high yields. Haematococcus pluvialis and Chlorella zofingiensis, two important carotenoid producers, were selected for co-culture cultivations to utilize the unique advantages of both organisms. A co-culture production process was investigated in terms of the effects of organic carbon source, co-cultivation method, and light intensity on carotenoid production.RESULTS: The addition of 5 g L−1 glucose resulted in a growth rate of 0.60 day−1 for H. pluvialis and 0.59 day−1 for C. zofingiensis, which were higher than those for other carbon sources tested and the control group. Incremental increase of light intensity instead of direct increase to 170 µE m−2s− prevented cell loss in both cultures. Co-cultivation based on cell numbers (60% H. pluvialis and 40% C. zofingiensis) prevented population domination of one microalgae over the other. The biomass production rate of the co-culture was higher (0.61 g L−1 day−1) in glucose-enriched medium. The total carotenoid content of the co-culture in the control culture was higher (0.83 mg total carotenoids g−1 cell) than that obtained in glucose-enriched medium (0.54 mg total carotenoids g−1 cell) but not as high as the amounts reached in mono-cultures.CONCLUSION: Total carotenoid content of the mono-cultures gave higher yields in standard bold basal medium (BBM). Preliminary high performance liquid chromatography (HPLC) studies indicated a variation in the amounts of astaxanthin isomers produced. Further studies are in progress to determine the effects of carbon-enriched media and co-cultivation on the type of isomers and caretenoids produced. Copyright © 2010 Society of Chemical Industry
Journal of Chemical Technology & Biotechnology 02/2011; 86(3):414 - 420. · 2.17 Impact Factor
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ABSTRACT: Haematococcus pluvialis Flotow is used in the aquaculture, pharmaceutical and cosmetic industries. The aim of this study was to compare the effect of various stress media and high light intensities on astaxanthin accumulation. The experimental design was achieved by four different stress media and two different light intensities for 14 days of induction period. The astaxanthin concentrations of 29.62 mg g(-1) and 30.07 mg g(-1) were obtained in distilled water with CO(2) and N-free medium, respectively, with no significant difference between them at 546 micromol photons m(-2)s(-1). Because of the morphological changes of H. pluvialis, microscopic observation was considered during the induction period to facilitate the selection of stress medium. It was clear that the rate of astaxanthin accumulation was much faster in distilled water with the addition of CO(2). The main point is that, this medium is more economical than others, especially for the large-scale productions.
New Biotechnology 09/2009; 26(3-4):199-204. · 2.76 Impact Factor
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ABSTRACT: The objectives of the present study on the growth of Haematococcus pluvialis were to indicate the effects of a long-term semi-continuous cultivation, sterilization, carbon dioxide, and different culture media by using artesian well water. This investigation was an enterprise in order to commercialize the production economically. When the effect of CO(2) was investigated in basal culture medium, the influence of sterilization was also researched in Rudic's culture medium in vertical panel-type photobioreactors for 31 days of semi-continuous cultivation. The maximum cell concentration of 10.55 x 10(5) cells ml(-1), which corresponds to the growth rate of 0.271 day(-1) with the areal productivity of 3.531 g m(-2) day(-1), was found in non-sterilized RM medium on the 24th day of the third run of semi-continuous cultivation at a renewal rate of 50% in a vertical panel-type photobioreactor.
Applied biochemistry and biotechnology 05/2009; 160(3):764-72. · 1.94 Impact Factor
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ABSTRACT: A Haematococcus pluvialis strain isolated from the ruins of Ephesus in Turkey was investigated as regards its adaptation to laboratory conditions and maximum growth rate. In the first stage of the experiment, the growth of H. pluvialis was compared in common culture media. Furthermore, in an effort to minimize the culture costs, the second stage of the experiment compared the growth rate in the culture medium selected in the first stage with that in commercial plant fertilizers. The results demonstrated that the maximum cell concentration of 0.90 g/l, corresponding to a growth rate of 0.150 d(-1), was found with an N-P-K 20:20:20 fertilizer under a light intensity of 75 micromol photons m(-2) s(-1) on the 12th day of cultivation.
Journal of Microbiology and Biotechnology 04/2007; 17(3):393-7. · 1.38 Impact Factor
