ABSTRACT: The long-term quality of life of adolescents with chronic rhinosinusitis improved significantly after functional endoscopic sinus surgery (FESS), and endoscopic sinus surgery showed certain benefits for adolescent patients. The selection of appropriate patients may further improve the surgical outcomes and quality of life.
To analyze the long-term quality of life in adolescents (12-18 years) with chronic rhinosinusitis after endoscopic sinus surgery, and to evaluate the value of endoscopic sinus surgery in adolescent patients.
From 2003 to 2008, 729 adolescents with chronic sinusitis underwent endoscopic sinus surgery in our department; 270 of these patients were included in the study. Their quality of life was assessed before and within 3-8 years after the surgery using the SNOT-20 scale and was compared with that of healthy individuals of the same age.
The SNOT-20-based assessment showed that the overall quality of life differed significantly before and after surgery (p = 0.000) and that some symptoms (dizziness, sense of facial oppression, sleep difficulty, embarrassment, and fatigue) had no significant differences before and after surgery (p > 0.05). Preoperative and postoperative symptoms (dizziness, sense of facial oppression, sleep difficulty, embarrassment, and fatigue) showed no significant differences between the healthy population and treated patients (p > 0.05).
Acta oto-laryngologica 06/2012; 132(8):798-802. · 0.98 Impact Factor
ABSTRACT: To address the question if apurinic/apyrimidinic endonuclease/redox factor 1 (APE/Ref-1) involved in preventing spiral ganglion cells oxidative damage after oxidative stress.
Primary cultured rat spiral ganglion cells were infected with the adenovirus containing APE/Ref-1 for 48 h, then treated with H2O2 (0, 10, 25, 50, 100, 300 micromol/L) for 1 h, and finally changed back into normal medium. Western blot were used to detect the level of APE/Ref-1 protein in the infected cells to ensure APE/Ref-1 over expression as a result of adenovirus infection. The cell viability was determined by MTT and the apoptosis of spiral ganglion cells was determined by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).
Western blot showed that infection of adenovirus resulted in APE/Ref-1 over expression in the spiral ganglion cells. Over expression of APE/Ref-1 significantly improved cell viability in cultures treated with different concentration H2O2 from 50 to 300 micromol/L However, the apoptosis of cells was significantly inhibited.
Over expression of APE/Ref-1 could protect spiral ganglion cells from oxidative damage.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 11/2008; 43(10):773-7.
ABSTRACT: To observe the effects of different shear stresses on the morphological changes in cochlear microvascular endothelial cells cultured in vitro so as to enrich the mechanism of the blood-labyrinth barrier.
The morphological photos of cochlear microvascular endothelial monolayer cells were obtained with the hydrodynamic system design and the morphological parameters such as Pyx and Q were detected.
Through digestion with collagenase type I, the monolayer cells of cochlear microvascular endothelial monolayer were obtained. As to cochlear microvascular endothelial cells in guinea pigs, no morphological changes of the cells were found when shear stresses of 0. 0883 Pa acting for 24 h. When shear stress was 0.1184 Pa acting for 8 h, compliant changes from former disorderliness into orderliness happened following the direction of the flowing liquid of the cellular morphology. The changing tendency was in a time-dependent manner.
Cochlear microvascular endothelial cells in guinea pigs after the effect of shear stress are morphologically different from statically cultured endothelial cells. Range of the shear stress that cochlear microvascular endothelial cells can tolerate is little.
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery 03/2007; 42(2):135-7.
ABSTRACT: To study the composition of microecosystem in ostiomeatal complex on adults and its changes via functional endoscopic sinus surgery.
Two hundred adults were grouped based on pre- or postoperation as well as its type of sinusitis. Swab speciemens were taken from three different sites of ostiomeatal complex in each individual, cultivations and identifications of aerobes, anaerobes and fungi were performed simultaneously; the microecosystem of OMC was analysesed.
All aerobes belonged to thirteen genera thirty species, anaerobes were three species and three major species of fungus were cultured, most of them have none or less virulence; statistically, none significance were shown between differences of type I and II (P > 0.05); as to aerobes, difference between preoperative group and control group had significance (P < 0.01); culture rate descended postoperatively while the difference had no significance compared to preoperative group (P > 0.05); difference of rate of same aerobic culture from three sites (RSAC) between preoperative and control group was significant (P < 0.01), while wasn't between postoperative group and control one (P > 0.05). Taking fungi into account, differences among all groups had no significance (P > 0.05); anaerobes were rare in all groups.
Being a microecosystem under normal condition, ostiomeatal complex is relatively clean and exists as a whole space; while chronic inflammation separate different parts of which from each other with multimicrobial existence; FESS reverses the separation of the microecosystem in OMC and rebuild it as a whole again, thus promote the recovery.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 05/2005; 19(8):358-60.