Yuko Takayama

Publications (2)3.58 Total impact

• Article: Mice lacking alpha1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors.

  Takashi Kudo, Takashi Fujii, Shiro Ikegami, Kaoru Inokuchi, Yuko Takayama, Yuzuru Ikehara, Shoko Nishihara, Akira Togayachi, Satoru Takahashi, Kouichi Tachibana, Shigeki Yuasa, Hisashi Narimatsu
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  ABSTRACT: The 3-fucosyl-N-acetyllactosamine [Lewis x (Le(x)), CD15, SSEA-1] carbohydrate structure is expressed on several glycolipids, glycoproteins, and proteoglycans of the nervous system and has been implicated in cell-cell recognition, neurite outgrowth, and neuronal migration during development. To characterize the functional role of Le(x) carbohydrate structure in vivo, we have generated mutant mice that lack alpha1,3-fucosyltransferase IX (Fut9(-/-)). Fut9(-/-) mice were unable to synthesize the Le(x) structure carried on glycoproteins and glycolipids in embryonic and adult brain. However, no obvious pathological differences between wild-type and Fut9(-/-) mice were found in brain. In behavioral tests, Fut9(-/-) mice exhibited increased anxiety-like responses in dark-light preference and in elevated plus maze tests. Immunohistochemical analysis showed that the number of calbindin-positive neurons was decreased in the basolateral amygdala in Fut9(-/-) mice. These observations indicated that the carbohydrates synthesized by Fut9 play critical roles in functional regulations of interneurons in the amygdalar subdivisions and suggested a role for the Le(x) structure in some aspects of emotional behavior in mice.
  Glycobiology 02/2007; 17(1):1-9. · 3.58 Impact Factor
• Article: Mice lacking {alpha}1,3-fucosyltransferase IX demonstrate disappearance of Lewis x structure in brain and increased anxiety-like behaviors

  Takashi Kudo, Takashi Fujii, Shiro Ikegami, Kaoru Inokuchi, Yuko Takayama, Yuzuru Ikehara, Shoko Nishihara, Akira Togayachi, Satoru Takahashi, Kouichi Tachibana, Shigeki Yuasa, Hisashi Narimatsu
  [show abstract] [hide abstract]
  ABSTRACT: The 3-fucosyl- N -acetyllactosamine (Lewis x (Lex), CD15, SSEA-1) carbohydrate structure is expressed on several glycolipids, glycoproteins, and proteoglycans of the nervous system and has been implicated in cell-cell recognition, neurite outgrowth, and neuronal migration during development. To characterize the functional role of Lex carbohydrate structure in vivo , we have generated mutant mice that lack α1,3-fucosyltransferase IX (Fut9-/-). Fut9-/- mice were unable to synthesize the Lex structure carried on glycoproteins and glycolipids in embryonic and adult brain. However, no obvious pathological differences between wild-type and Fut9-/- mice were found in brain. In behavioral tests, Fut9-/- mice exhibited increased anxiety-like responses in dark-light preference and in elevated + maze tests. Immunohistochemical analysis showed that the number of calbindin-positive neurons was decreased in the basolateral amygdala in Fut9-/- mice. These observations indicated that the carbohydrates synthesized by Fut9 play critical roles in functional regulations of interneurons in the amygdalar subdivisions and suggested a role for the Lex structure in some aspects of emotional behavior in mice.