D Debnath

Publications (5)3.45 Total impact

• Article: Haemato-biochemical responses and induction of HSP70 to dietary phosphorus in Catla catla (Hamilton) fingerlings.

  K Sukumaran, A K Pal, N P Sahu, R S Dalvi, D Debnath
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  ABSTRACT: A feeding trial of 120 days was conducted to study the effect of graded levels of dietary phosphorus on haematology, serum protein concentrations and HSP70 expression in fingerlings of the Indian major carp, Catla (Catla catla). Eight isonitrogenous and isoenergetic purified diets were formulated to contain graded levels of dietary phosphorus (dP), i.e., T(1), 0.1%; T(2), 0.3%; T(3), 0.5%; T(4), 0.7%; T(5), 0.9%; T(6), 1.1%; T(7), 1.3%; or T(8), 1.5%. Four hundred and eighty fish (average weight 4.23 +/- 0.016 g) were equally distributed into 24 tanks forming eight treatments with three replicates each. The fish were fed daily at the rate of 3.5% body weight in two instalments. At the end of feeding trial fish were sampled to study total RBC and WBC count, haemoglobin, serum lysozyme activity, serum total protein, albumin (A), globulin (G) concentration and HSP70 expression. Total RBC count, haemoglobin concentration and serum lysozyme activity did not vary significantly in response to different dietary phosphorus concentrations. Total WBC count was found to be significantly (P < 0.05) higher in T(1 )relative to all other treatments. Serum albumin and A/G ratio was found to be significantly lower in fish of T(1) and T(2) in relation to T(7) group (P < 0.05). Serum globulin and total protein levels remained unaffected by variations in dietary phosphorus. HSP70 expression was observed in T(1) group (0.1% dP) in gills and brain tissue, but not in liver and muscle tissues. No HSP70 expression was observed in fish of T(4) (0.7% dP) and T(8) (1.5% dP) treatments. These prima facie results suggest that dietary phosphorus had only minor influence on the haemato-biochemical parameters studied; however dietary phosphorus deficiency caused organ specific induction of HSP70 in catla fingerlings.
  Fish Physiology and Biochemistry 12/2008; 34(4):299-306. · 1.53 Impact Factor
• Article: Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels.

  D Debnath, A K Pal, N P Sahu, S Yengkokpam, K Baruah, D Choudhury, G Venkateshwarlu
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  ABSTRACT: Labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in India. Digestive enzyme response and metabolic profile of fingerling L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. After 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. Amylase, lipase and alkaline phosphatase (ALP) activities were not influenced by the dietary protein, but proteolytic and acid phosphatase (ACP) activities varied (P<0.05) between the treatments. Proteolytic activity showed a second order polynomial relationship with dietary crude protein (CP) as Y = 0.0734X(2) + 4.937X - 68.37, r(2)=0.97. A positive correlation was observed between dietary CP and amylase (r(2)=0.78). All the metabolites except muscle glucose showed significant change corresponding to the dietary protein levels. Glucose and glycogen levels corresponded to the dietary carbohydrate levels. Muscle and plasma pyruvic acid increased as the crude protein in the diet increased, whereas liver pyruvic acid showed the opposite trend. Muscle protein content was not affected by dietary CP. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP fed group. It is concluded that proteolytic activity and ACP are the major digestive enzymes responsive to dietary CP in L. rohita fingerlings. Considering the cost effectiveness of the diet, and based on liver and plasma free amino acid levels and plasma protein fractions, 30% crude protein is recommended as the optimal dietary protein for L. rohita fingerlings.
  Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology 01/2007; 146(1):107-14. · 1.92 Impact Factor
• Article: Thermal tolerance and metabolic activity of yellowtail catfish Pangasius pangasius (Hamilton) advanced fingerlings with emphasis on their culture potential

  D. Debnath, A.K. Pal, N.P. Sahu, K. Baruah, S. Yengkokpam, T. Das, S.M. Manush
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  ABSTRACT: Thermal tolerance and metabolic activity of Pangasius pangasius fingerlings (22.7 ± 1.5 g) was evaluated in terms of critical thermal maximum (CTmax), critical thermal minimum (CTmin), lethal thermal maximum (LTmax), lethal thermal minimum (LTmin) and rate of oxygen consumption after acclimating the fingerlings at 30, 34 and 38 °C for 30 days. CTmax (mean ± S.E.) (42.68 ± 0.03, 43.67 ± 0.05, 44.05 ± 0.12), CTmin (12.37 ± 0.03, 14.48 ± 0.03, 17.22 ± 0.09), LTmax (42.95 ± 0.02, 44.35 ± 0.02, 44.53 ± 0.03) and LTmin (11.75 ± 0.02, 12.52 ± 0.03, 14.35 ± 0.02) increased significantly (p < 0.05) with increasing acclimation temperatures. Oxygen consumption rate increased significantly (p < 0.05) with increasing acclimation temperature from 30 to 34 °C, but the change was not significant from 34 to 38 °C. Temperature quotient (Q10) of acclimated fish (2.47) was lesser than the non-acclimated fish (3.27). The area of thermal polygon over the range of acclimation temperatures (30–38 °C) of P. pangasius was calculated as 231 °C2. Overall results indicate that thermal tolerance and metabolic activity in P. pangasius is dependent on acclimation temperature and is comparable to Indian major carps, suggesting their culture potential in tropical region.
  Aquaculture. 258:606-610.
• Source

  Available from: Kartik Baruah

  Book: Dietary Phytase: an ideal approach for a cost effective and low-polluting aquafeed

  K. Baruah, N.P. Sahu, A.K Pal, D. Debnath
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  ABSTRACT: Global fishmeal production from wild-catch sources cannot continue to increase indefinitely; suitable alternatives have to be found for sustainable aquaculture. Plant-based aquafeed seems to be the ideal alternative to this, but has its own limitations. Plant ingredients are rich in phytic acid, which reduces the bioavailability of nutrients like minerals and protein to the fish, thereby causing aquaculture pollution. Dietary phytase treatment reduces the aquaculture pollution by improving the bioavailability of nutrients, and reduces the feed cost as evident from poultry and piggery. Phytase activity is highly dependent upon the pH of the gut. Unlike mammals, fish are either gastric or agastric, and hence, the action of dietary phytase varies from species to species. In this article, the authors attempt to summarise various effects of phytase on nutrient utilization, growth of fish and aquatic pollution.
• Article: Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels

  D. Debnath, A.K. Pal, N.P. Sahu, S. Yengkokpam, K. Baruah, D. Choudhury, G. Venkateshwarlu
  [show abstract] [hide abstract]
  ABSTRACT: Labeo rohita, commonly called rohu is one of the most important fish species for aquaculture in India. Digestive enzyme response and metabolic profile of fingerling L. rohita to different dietary crude protein (CP) levels (viz. 25, 30, 35 and 40%) were studied in an attempt to optimize a practical diet formulation for this species. After 45 days of feeding, activity of digestive enzymes and metabolite concentrations were assayed. Amylase, lipase and alkaline phosphatase (ALP) activities were not influenced by the dietary protein, but proteolytic and acid phosphatase (ACP) activities varied (P < 0.05) between the treatments. Proteolytic activity showed a second order polynomial relationship with dietary crude protein (CP) as Y = 0.0734X2 + 4.937X − 68.37, r2 = 0.97. A positive correlation was observed between dietary CP and amylase (r2 = 0.78). All the metabolites except muscle glucose showed significant change corresponding to the dietary protein levels. Glucose and glycogen levels corresponded to the dietary carbohydrate levels. Muscle and plasma pyruvic acid increased as the crude protein in the diet increased, whereas liver pyruvic acid showed the opposite trend. Muscle protein content was not affected by dietary CP. Protein fractions in plasma (total protein, albumin and globulin) showed maximum values in 30% CP fed group. It is concluded that proteolytic activity and ACP are the major digestive enzymes responsive to dietary CP in L. rohita fingerlings. Considering the cost effectiveness of the diet, and based on liver and plasma free amino acid levels and plasma protein fractions, 30% crude protein is recommended as the optimal dietary protein for L. rohita fingerlings.
  Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology.