Xiao-Hong Gao

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (4)9.79 Total impact

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    ABSTRACT: To compare the estradiol production and P450 aromatase messenger RNA (mRNA) expression of cultured luteinized granulosa cells and the effect of letrozole on these parameters between women with and without endometriosis. In vitro assays. Reproductive medical center. Patients undergoing in vitro fertilization (IVF): 23 patients with endometriosis and 19 controls without endometriosis. Luteinized granulosa cells examined for estradiol levels and P450 aromatase mRNA expression in conditioned media at different letrozole concentrations of 0.0, 0.1, 1.0, and 10.0 μmol/L. Estradiol levels of the conditioned medium measured with an enzyme-linked immunosorbent assay (ELISA) kit and P450 aromatase mRNA expression determined by quantitative reverse transcription-polymerase chain reaction (QT RT-PCR). The estradiol concentration of the conditioned media and P450 aromatase mRNA expression in the endometriosis group were statistically significantly lower than that of the control group, irrespective of letrozole concentrations. A statistically significant reduction of these two parameters was observed in both endometriosis and control groups at letrozole concentrations of 1 μmol/L and 10 μmol/L, but there were no statistically significant differences between letrozole concentrations of 1 μmol/L and 10 μmol/L. The number of high-quality embryos in the endometriosis group was statistically significantly lower than that of the control group. Lower estradiol production and P450 aromatase mRNA expression of cultured granulosa cells were found in women with endometriosis. Letrozole in the conditioned media further reduced these parameters.
    Fertility and sterility 05/2012; 98(1):131-5. · 3.97 Impact Factor
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    ABSTRACT: To investigate the effects of ovarian stimulation on endometrial receptivity in the peri-implantation phase. Comparison of integrin beta3 and leukemia inhibitory factor (LIF) expression in endometrial biopsies from moderate responders and high responders with temporally matched natural cycles. Clinical and experimental research. Patients with cancelled embryo transfer were evaluated. Moderate responders were defined as E2 5,000-15,000 pmol/L on the day of hCG administration (group B, n = 7). High responders were defined as E2 >15,000 pmol/L on the day of hCG administration (group C, n = 8). Healthy and fertile volunteers were used as natural controls (group A, n = 10). Endometrial biopsy on the day of LH+7/hCG+7. Immunohistochemistry of integrin beta3 and LIF and imaging analysis. Endometrial integrin beta3 and LIF expressions in the peri-implantation phase were significantly lower in stimulated cycles (including both moderate and high responders) compared to natural controls. Expression of LIF in glandular epithelium in high responders was lower than that in moderate responders. These data demonstrated that suggested markers of endometrial receptivity were reduced in stimulated cycles compared with natural cycles, and more so in high response cycles compared with moderate response cycles.
    Fertility and sterility 05/2008; 89(5 Suppl):1357-63. · 3.97 Impact Factor
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    ABSTRACT: The study was aimed at investigating the effects of ovarian high response during controlled ovarian hyperstimulation (COH) on implantation and pregnancy outcome in fresh IVF/ICSI cycles, and subsequent frozen-thawed embryo transfer (FET) cycles. An analysis of 1,196 cycles using a long protocol with GnRHa and rFSH was performed. A serum oestrial level (peak E(2)) was obtained on the day of hCG administration, and patients were grouped by peak E(2) percentile distribution into 3 groups. Normal responder was set as cut-off concentrations between percentile (P)25 and P75 (Group A: 1,199-3,047 pg/ml, 595 cycles). Moderate high responders were classified as peak E(2) between P75-P90 (Group B: 3,048-4,127 pg/ml, 180 cycles). For the high response group, the E(2) cut-off concentration was set as P90 and above (Group C: >or=4,128 pg/ml, 119 cycles). Oocyte/embryo parameters and clinical outcomes were compared among the 3 groups in fresh cycles and subsequent FET cycles. Comparisons between groups revealed no difference in the quality of oocyte retrieved and in fertilisation rates. Group C showed decreased trends in implantation and pregnancy rates compared with Group A, but statistical significance was reached only for the difference in implantation rates. Implantation and pregnancy rates in FET cycles were similar among the 3 groups. High serum estrogen levels were detrimental to implantation, but not to the quality of oocytes, which may be due to an adverse effect on endometrial receptivity in COH cycles.
    Acta Obstetricia Et Gynecologica Scandinavica 02/2007; 86(7):849-54. · 1.85 Impact Factor
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    ABSTRACT: ObjectiveTo investigate effects of ovarian high response on endometrial mucin-1 (MUC1) and pinopode in peri-implantation phase in controlled ovarian hyperstimulation (COH) cycles.MethodsOvarian high response was defined as serum E2 > 15 000 pmol/L on the day of hCG administration in COH cycle using GnRH agonist and recombinant FSH (n=8). Healthy and fertile women were used as the natural control (n=10). Endometrial biopsies were performed on the day of LH+7/hCG+7. Pinopode formation was observed by scanning electron microscope. Expression of MUC1 was detected with quantitative Real-time PCR and immunohistochemistry.ResultsIn high response group, the lumen surface was covered with variant pinopodes and microvillous. The expression of MUC1 mRNA in high response group was lower than that in the natural control (P < 0.05). Immunostaining for MUC1 protein in glandular and luminal epithelium in high response group was lower than that in the natural control (P < 0.05).ConclusionAsynchronized pinopode appearance and lower expression of MUC1 during peri-implantation period were the characteristics of endometrium in high response group, which may provide a clue of decreased endometrial receptivity in the supraphysiological hormone milieu.
    Journal of Reproduction and Contraception. 01/2007;

Publication Stats

27 Citations
9.79 Total Impact Points

Institutions

  • 2007
    • Shanghai Jiao Tong University
      Shanghai, Shanghai Shi, China