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ABSTRACT: Pet-rat bite fever is a relatively rare disease consecutive to a rat bite or scratch. The authors report a case of septic arthritis following a pet rat bite. Streptobacillus moniliformis was identified in the knee synovial fluid and identified by 16S rRNA sequencing. This is a rapid and efficient tool for identification of fastidious bacterium. The patient was cured by an amoxicillin treatment.
Médecine et Maladies Infectieuses 06/2007; 37(5):293-4. · 0.72 Impact Factor
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ABSTRACT: Accurate identification of bacterial isolates is an essential task in clinical microbiology. Phenotypic methods are time-consuming and either fail to identify some bacteria such as Gram-positive rods entirely or at least fail to do so in some clinical situations. 16S rDNA sequencing is a recent method of identification which offers a useful alternative. In this study, we investigate the usefulness of this method for identifying a range of bacteria in a clinical laboratory under routine conditions. Over a period of 30 months, 683 isolates were obtained from clinical specimens, sequenced and analysed. For 568 of these isolates (83.1%), the sequence provided species level identification. For 108 isolates (15.8%), the identification was limited to the genus level, and for 7 isolates (1%), the sequence remained unidentifiable by 16S rDNA sequence analysis. For the isolates identified only to the genus level, the 16S rDNA approach failed to identify bacteria to the taxonomic level for 3 reasons: failure to differentiate between species in 72 isolates (66%), the lack of any closely related sequence in the database for 15 isolates (13.8%) and the presence of more than 1% of undetermined position in the sequence for 13 isolates (12%).
Journal of Microbiological Methods 01/2007; 67(3):574-81. · 2.09 Impact Factor
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ABSTRACT: We report here the case of a 30-year-old woman presenting a disturbed lipid profile since her childhood. The rarity of this pathology (Anderson' disease or chylomicron retention disease) and its recent recognition explain its late detection in this case. The description of the biochemical profile is interesting especially the low level of vitamin A and E which explains the severity of the disease. A vitamin enriched-diet may be useful.
Annales de biologie clinique 62(3):330-3. · 0.34 Impact Factor
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S Mignard,
P-Y Donnio,
J Vaucel,
M-L Abalain,
M Anguill,
J-P Aubry,
L Desbordes,
M-J Dufour,
J-Y Esvant,
F Gangneux, [......],
A LeMeur,
J Minet,
O Morvan,
P Plessis,
P Pouedras,
J Rome,
D Tandé,
A Valogne,
C Voisine,
J-F Ygout
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ABSTRACT: At this time, many antibiotics have decreased activity against Streptococcus pneumoniae, a major agent of infectious disease. In this study, we evaluated antibiotic susceptibility and serogroups of strains isolated from bacteraemia, meningitis and acute otitis media in adults and children over the 1997-2003 period in Brittany, France. In 2003, 62% of the isolates were not susceptible to penicillin and 11% were fully resistant. The prevalence of erythromycin resistance was 63%. Resistance rates were higher among isolates recovered from children than adults. Serogroups 19 and 14 were the most frequently isolated, especially the 19 one among children. The emergence of this serogroup might be a consequence of the use of heptavalent conjugate vaccine introduced in 2001 in France. Future surveillance after vaccination will be needed to detect emerging serogroups and resistance among S. pneumoniae.
Pathologie Biologie 53(8-9):457-62. · 1.53 Impact Factor
