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ABSTRACT: We have demonstrated a channel-spacing- and wavelength-tunable multiwavelength fiber ring laser based on a semiconductor optical amplifier and a tunable comb filter. By employing the comb filter, multiwavelength operation of up to 23 laser lines with the signal-to-noise ratio over 25 dB and 0.8-nm wavelength spacing was demonstrated at room temperature. The results show that the multiwavelength operation is even and stable. Furthermore, the shift of lasing wavelength and wavelength spacing were continuously controllable in this system
IEEE Photonics Technology Letters 12/2006; · 2.19 Impact Factor
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J.H. Chien,
D.S. Lee,
W.P. Chou,
P.Y. Wang,
C.R. Yang,
M.H. Wu,
C.Y. Tsai,
T.L. Chang, Y.W. Lee,
Y.T. Cheng,
P.H. Chen
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ABSTRACT: The current real-time PCR (polymerase chain reaction) platforms, which can detect and quantify several target DNA simultaneously, are equipped with discrete optics and detectors for different fluorescence wavelengths. However, the optical loss, due to the different lengths of the channels for several dyes, lowers the performance of fluorescence detection. Especially on the PCR platforms of lab-on-a-chip system, for the dispersion of the fluorescence in the micro fluidic channels, the received fluorescence is much lower than the emitted. To enhance the received intensity on the detection system is therefore a critical issue. The proposed fluorescence detection system, composing of an ultra-sensitive spectrometer, can provide continuous wavelength detection and can be employed for multiple DNA quantification and genotyping in a single reaction. For the tests to the genotyping ability, the melting temperatures of B type HBV and C type HBV can be distinguished by the difference of 1.1degC.The test results in this research show the same degree of sensitivity for DNA quantification and reproducibility within five intra assay samples as compared with a commercial one
Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on; 02/2006
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J.H. Chien,
C.Y. Tsai,
Y.T. Cheng,
C.R. Yang,
P.Y. Wang,
T.L. Chang,
D.S. Lee,
C.W. Chang,
W.P. Chou,
C.C. Chiang, Y.W. Lee,
P.H. Chen
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ABSTRACT: Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the microcapacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radiofrequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection
Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on; 02/2006
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ABSTRACT: This study employs a computational fluid dynamics (CFD) software package to obtain a two-phase three-dimensional flow field and conjugate heat transfer results for a disk-shape miniature heat pipe (DMHP) that is used to dissipate heat from a laser diode. A mixture model is introduced into the governing equations for calculating the quality of mixture in the two-phase flow field. Predicted thermal resistances are presented at three different fluid charge volumes of 18%, 55%, and 92% at different heating powers of the laser diode. The predicted thermal resistance of the DMHP agrees well with measured results given in our previous study [1].
Numerical Heat Transfer Part a-Applications. 01/2006; 49(1):25-45.
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ABSTRACT: The poloidal field magnet system of the KSTAR consists of three pairs of coils using the cable-in-conduit conductors cooled by forced super-critical helium. The conductor of PF5 is Nb<sub>3</sub>Sn superconductor with Incoloy 908 conduit, and that of PF6 and PF7 is NbTi superconductor with SS 316LN. The PF coils are self-supporting with regard to radial loads, and they are arranged symmetrically with respect to the equatorial plane. PF5 coil is connected to the TF coil structure at eight points by hinges, while PF6 and PF7 coils are connected at sixteen points by flexible plates to allow relative radial movements. In order to investigate the structural integrity, structural analysis has been conducted including buckling and fatigue analyses. The major design loads are dead weight, assembly loads, thermal load due to cool down, and electromagnetic loads under the critical load conditions. It is found that the PF magnet structures can safely withstand all operating conditions. In addition, AISI 316LN can be substituted for JJ1 as the material of the PF coil structures.
IEEE Transactions on Appiled Superconductivity 07/2004; · 1.04 Impact Factor
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ABSTRACT: The pump source was a continuous-wave external-cavity diode laser followed by an erbium-doped fiber amplifier. The pump wavelength was tunable from 1530 nm to 1560 nm, and the pump power was 26 mW. We focused the pump beam to the center of the LiNbO<sub>3</sub> aperiodic optical superlattice.
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the; 02/2002
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J H Chien,
C Y Tsai,
Y T Cheng,
C R Yang,
P Y Wang,
T L Chang,
D S Lee,
C W Chang,
W P Chou,
C C Chiang, Y W Lee,
P H Chen
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ABSTRACT: Different from traditional detection methods including polymerase chain reaction (PCR) based assays and enzyme-linked immunosorbent assays (ELISA) which are expensive and time consuming for biochemical analysis, in our study, a DNA chip assay using gold nanoparticle (AuNP) immunological amplification method and RFID communication platform were applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the capacitor locates. Then the target DNA (tDNA) and probe DNA (pDNA) with a AuNP at the 3' end were added to form several layers of AuNP linked structures. Since the permittivity of the medium in which the capacitor is located changed due to the AuNP-linking structures between the gaps of the micro capacitor, the resonant frequency of series LC circuit shifted immediately. The lab-on-a-chip proposed in this study was power supplied by the radio frequency identification (RFID) system. The shift of the LC resonant frequency determined whether the tDNA existed. Thus the detection chip needed no any other internal power supply or expensive instruments for electrical signal measurement. The study provides a simple, fast and convenient platform for biochemical detection.
