Jingjing Hu

Publications (3)8.74 Total impact

• Article: Sensitive and rapid LC-ESI-MS method for the determination of trimetazidine in human plasma.

  Li Ding, Bin Gong, Xiaoxiang Chu, Jingjing Hu, Hen Zheng
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  ABSTRACT: A sensitive method, based on liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS), was developed for the determination of trimetazidine in human plasma. Buflomedil was used as the internal standard (IS). Plasma samples were extracted with a mixture of cyclohexane-diethyl ether (1:1, v/v) and the analytes were chromatographically separated on a phenomenex Luna 5 mu C18 (2) 100A HPLC column with a mobile phase of 10 mM ammonium acetate buffer solution containing 0.1% acetic acid-methanol (45:55, v/v). The electrospray ionization was employed in a single quadrupole mass spectrometer for the analytical determination. The lower limit of quantification (LLOQ) was 0.5 ng/ml for trimetazidine and the measuring ranges were from 0.5 to 200 ng/ml. The intra- and inter-run standard deviation was less than 4.1% and 7.8%, respectively. The method was successfully applied to study the pharmacokinetics of trimetazidine in healthy Chinese volunteers.
  Journal of Pharmaceutical and Biomedical Analysis 07/2007; 44(2):526-31. · 2.97 Impact Factor
• Article: Determination of raltitrexed in human plasma by high performance liquid chromatography-electrospray ionization-mass spectrometry.

  Jingjing Hu, Li Ding, Qinxin Song, Ying Gao, Shukui Qing
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  ABSTRACT: A sensitive high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) assay was developed to determine raltitrexed in human plasma. After addition of benazeprilat as the internal standard (IS), methanol was used to produce a protein-free extract. Chromatographic separation was achieved with a Zorbax SB-C18 column (Narrow-Bore 2.1 mmx150 mm, 5-microm) using a mobile phase of acetonitrile-water containing 0.1% formic acid and 2% methanol (21.9:78.1, v/v). Raltitrexed was determined with electrospray ionization-mass spectrometry. HPLC-ESI-MS was performed in the selected ion monitoring (SIM) mode using target ions at [M+H]+ m/z 459.1 for raltitrexed and [M+H]+ m/z 397.1 for IS. Calibration curves were linear over the range of 2.0-3000 ng/ml. The lower limit of quantification was 2.0 ng/ml. The intra- and inter-batch variability values were less than 6.7% and 10.3%, respectively. The mean plasma extraction recovery of raltitrexed was in the range of 85.2-91.1%. The method was successfully applied to determine the plasma concentrations of raltitrexed in eight Chinese colorectal cancer patients.
  Journal of Chromatography B 07/2007; 853(1-2):147-53. · 2.89 Impact Factor
• Article: Sensitive HPLC-APCI-MS method for the determination of cyclovirobuxine D in human plasma.

  Li Ding, Jingjing Hu, Meng Jiang, Ningning Xiong
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  ABSTRACT: A sensitive high performance liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry (HPLC-APCI-MS) assay for determination of cyclovirobuxine D (CVB-D) in human plasma using mirtazapine as internal standard (I.S.) was established. After adjustment to a basic pH with sodium hydroxide, plasma was extracted by ethyl acetate and separated by high performance liquid chromatography (HPLC) on a reversed-phase C(18) column with a mobile phase of 30 mM ammonium acetate buffer solution containing 1% formic acid-methanol (48:52, v/v). CVB-D was determined with atmospheric pressure chemical ionisation-mass spectrometry (APCI-MS). HPLC-APCI-MS was performed in the selected-ion monitoring (SIM) mode using target ions at [M+H](+)m/z 403.4 for CVB-D and [M+H](+)m/z 266.2 for I.S. Calibration curves were linear over the range 10.11-4044 pg/ml. The lower limit of quantification was 10.11 pg/ml. The intra- and inter-run variability values were less than 9.5 and 12.4%, respectively. The mean plasma extraction recovery of CVB-D was in the range of 85.3-92.8%. The method was successfully applied to determine the plasma concentrations of CVB-D in Chinese volunteers.
  Journal of Chromatography B 11/2006; 843(1):78-83. · 2.89 Impact Factor