Wenlong Luo

Publications (4)2.18 Total impact

• Article: Betulinic acid induces Bax/Bak-independent cytochrome c release in human nasopharyngeal carcinoma cells.

  Yang Liu, Wenlong Luo
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  ABSTRACT: Betulinic acid (BetA) is an effective and potential anticancer chemical derived from plants. BetA can kill a broad range of tumor cell lines, but has no effect on untransformed cells. The chemical also kills melanoma, leukemia, lung, colon, breast, prostate and ovarian cancer cells via induction of apoptosis, which depends on caspase activation. However, no reports are yet available about the effects of BetA on nasopharyngeal carcinoma (NPC), a widely spread malignancy in the world, especially in East Asia. In this study, we first showed that BetA can effectively kill CNE2 cells, a cell line derived from NPC. BetA-induced CNE2 apoptosis was characterized by typical apoptosis hallmarks: caspase activation, DNA fragmentation, and cytochrome c release. Overexpression of Bcl-2 and Bcl-xL could partially prevent apoptosis caused by BetA. Moreover, Bax was not activated during the induction of apoptosis. Bax/Bak knockdown and wild-type CNE2 cells showed the same kinetics of cytochrome c release. We then showed that BetA may impair mitochondrial permeability transition pores (mPTPs), which may partially contribute to cytochrome c release. These observations suggest that BetA may serve as a potent and effective anticancer agent in NPC treatment. Further exploration of the mechanism of action of BetA could yield novel breakthroughs in anti-cancer drug discovery.
  Molecules and Cells 04/2012; 33(5):517-24. · 2.18 Impact Factor
• Article: [Adenoid cystic carcinoma of ENT associated with hard lump: a report of 9 cases].

  Leiji Li, Wenlong Luo, Xiuni Gan, Lisha Su, Weidong Zeng
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  ABSTRACT: To assess the clinical and pathological features of the patients with ENT adenoid cystic carcinoma (ENTACC) who presented with hard lump, and to detect its prognosis. A retrospective analysis was performed on 9 patients with ENTACC by their clinical data. The period from the occurrence of primary symptoms to definite diagnosis was 4 years and 3 months on average. All patients were followed up from 6 months to 15 years. All of the cases relapsed after operation and radiotherapy. Five patients died, among which 3 died with lung metastases, 2 with local lymphatic metastases, 1 with bone metastases and 1 with encephalic diffusion. The other 4 cases still survive. The longest survival period with tumor was 6 years and 3 months. The pathological result of these 9 cases were all salivary gland derived adenoid cystic carcinoma of solid type. It was mainly characterized by the development of ENT hard lump. All of the patients should be diagnosed in time. Extensive excision and postoperative radiotherapy are needed at the first time. The tumor of solid histological pattern appears with much poorer prognosis compared with that of other histological pattern.
  Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 11/2006; 20(19):875-7.
• Article: [Preliminary clinical study of application of cyntridini triphosphatis and dexamethasone in treatment of sudden deafness].

  Wenlong Luo, Shixi Liu, Chuanyu Liang, Yang Liu, Cuiying Zhou, Qian Cao, Junbo Su
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  ABSTRACT: To investigate the effect of application of cytridini triphosphatis (CTP) combined with dexamethasone on sudden deafness. Two-hundred and seven patients (312 ears) with sudden deafness were randomly divided into CTP group (n = 159 ears) and control group (n = 153 ears). Besides intravenous administration of CTP, another treatments in CTP group were the same as that in control group including hyperbaric oxygen, vasodilator, energy preparation such as ATP, CoA and dexamethasone (DXM). The hearing threshold was (75.56 +/- 30.24) dB HL in CTP group and (72.50 +/- 40.50)dB HL in control group (P > 0.05) before treatment. The average value of the hearing threshold after treatment was decreased by (50.08 +/- 21.47) dB HL in CTP group and (19.45 +/- 19.12) dB in control group(P < 0.05), respectively. The application of CTP combined with DXM can significantly improve the effect of treatment on sudden deafness and CTP may enhance the recovery rate in the patients with sudden deafness, which was much higher than that treated by the traditional methods. However, the mechanism of the effect of CTP coupled with DXM is still unknown and further study is necessary.
  Zhonghua er bi yan hou ke za zhi 08/2002; 37(4):277-9.
• Conference Proceeding: Application of 3D-FDTD to the analysis of microstrip circuits including active nonlinear devices

  Wenlong Luo, Donglin Su, Shannwei Lu
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  ABSTRACT: In this paper, a 3D-FDTD method for the analysis of microwave circuits including active nonlinear devices is proposed. The relationship between electromagnetic fields and voltage sources is described according to Faraday's law, and then ports of microstrips are modeled by equivalent voltage sources. In order to connect active nonlinear devices to the voltage sources, equivalent circuits of the devices are employed. An example with a diode was analyzed and some reasonable time-domain results are obtained. The experimental studies are on going.
  Computational Electromagnetics and Its Applications, 1999. Proceedings. (ICCEA '99) 1999 International Conference on; 02/1999