[Show abstract][Hide abstract] ABSTRACT: Dead insects infected with fungi were collected from mountains and islands in several locations in Korea from 2003 to 2007. Morphological characteristics of the fungal fruiting bodies produced on the insect samples were examined. A total of 542 entomopathogenic fungal isolates were obtained from the insect samples and identified based on their morphological and cultural characteristics. Three species of Beauveria, eight species of Cordyceps and four species of Isaria were mainly isolated from the insect samples. The other entomopathogenic fungi isolated from the insect samples were Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces sp. and Verticillium sp.
[Show abstract][Hide abstract] ABSTRACT: Fungal floras were investigated for the lysimeter soil treated with municipal sewage sludge (MSS), pig manure compost (PMC), industrial sewage sludge (ISS), leather processing sludge (LS), and alcohol fermentation processing sludge (FS). Fungal populations were higher in the FS, ISS, LS, or MSS-treated soil than in the chemical fertilizer-treated soil. Isolated fungi from the sewage sludge were identified as Penicillium spp., Gliocladium spp., Acremonium spp., Trichoderma spp., Aspergillus spp., Blastomyces spp., and Phoma spp.
[Show abstract][Hide abstract] ABSTRACT: Mycelial growth of Pleurotus ostreatus isolates was examined on PDA media added with heavy metals to reveal effects of heavy metals on mycelial growth of the fungus. Cd and Cu strongly inhibited mycelial growth of three isolates of fungus tested. However, addition of Pb to growing media of white color mutant of fungus resulted in increase of the fungal growth rate. Addition of 2 mM Cr to the media resulted in increase of growth rate of the white color mutant and the dark color mutant of fungus. Mycelial growth rate of the white color mutant was relatively better than the other isolates on media added with Cr, Pb, Cu, or Mn, respectly. Tolerance of the white color mutans to heavy metals was higher than that of the black color mutant. It is suggested that tolerance of the white color mutant to heavy metals was not controled by color-related substance of the fungus.
[Show abstract][Hide abstract] ABSTRACT: A mesophilic bacterium, strain 15-52(T), was isolated from the rhizosphere of Chinese cabbage (Brassica campestris). On the basis of phenotypic and genotypic characteristics, the bacterium was identified as representing a novel species belonging to the genus Pedobacter. The strain is non-flagellated, non-spore-forming and grows at temperatures in the range 1-37 degrees C. Physiological tests of the strain showed the presence of oxidase, catalase, protease (gelatin and casein hydrolysis), beta-glucosidase and beta-galactosidase activities. The highest levels of 16S rRNA gene sequence similarity were found with respect to Pedobacter roseus CL-GP80(T) (97.3 %) and Pedobacter sandarakinus DS-27(T) (97.2 %). A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 15-52(T) is a member of the genus Pedobacter. DNA-DNA hybridization analysis revealed low levels of relatedness (<42.3 %) between the isolate and two phylogenetically related type strains, P. roseus KCCM 42272(T) and P. sandarakinus KCTC 12559(T). The DNA G+C content is 44.2 mol% and the predominant fatty acids are iso-C(15 : 0) (35.4 %), iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c (27.8 %) and iso-C(17 : 0) 3-OH (15.8 %). On the basis of these data, strain 15-52(T) represents a novel species of the genus Pedobacter, for which the name Pedobacter suwonensis sp. nov. is proposed. The type strain is 15-52(T) (=KACC 11317(T)=DSM 18130(T)).
International journal of systematic and evolutionary microbiology 03/2007; 57(Pt 3):480-4. · 2.11 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study was carried out to investigate the physiological activities of the ethanol extract from Gymnopilus spectabilis mycelium (EGM) and of the supernatant obtained from fermentation broth (SGB). The contents of polysaccharides, phenol compounds and total of EGM were found to be 80.14%, 3.5 mg/ml and 5.91%, respectively and those for SGB were 78.68%, 3.32 mg/ml and 3.28%, respectively. Both EGM and SGB exhibited dose-dependent nitrate-scavenging abilities at pH 1.2. In addition, both EGM and SGB on the autoxidation rate of the linoleic acid demonstrated powerful antioxidant activities at 1 mg/ml level. With respect to fibrolytic activity, EGM showed 1,180 unit/g, which was the same activity as streptokinase, while SGB was 1,011 unit/g. The angiotensin converting enzyme inhibition activity of EMG determined by both the normal and pretreatment methods were estimated to be 8.2% and 10.2%, respectively. However, SGB showed no corresponding activity. The growth inhibitory effects of EGM on AGS, A549, HeLa and NCTC cells were over 58.88%, respectively. And the growth inhibitory effects of the SGB on HeLa and NCTC cells were 44.92 and 76.76%, respectively. Also, EGM and SGB activated the components of the alternative complement pathway from 51 and 62% at the concentration of 100 mg/ml, The xanthine oxidase inhibition activities of EGM and SGB (1 mg/ml) were 9.53 and 16.92%, respectively.
[Show abstract][Hide abstract] ABSTRACT: A novel strain, R2A16-10(T), was isolated from greenhouse soil in Yeoju, Korea. The taxonomy of strain R2A16-10(T) was studied by using polyphasic methods. On the basis of 16S rRNA gene sequence analyses, strain R2A16-10(T) was found to be phylogenetically related to type strains of Dyella species (96.7-96.9 %), Frateuria aurantia DSM 6220(T) (96.5 %), Fulvimonas soli LMG 19981(T) (96.3 %) and Rhodanobacter species (94.9-95.7 %). Strain R2A16-10(T), which produced yellow-coloured colonies, was Gram-negative, rod-shaped (0.3-0.4x1.5-3.5 mum) and motile. The predominant fatty acids were 17 : 1 iso omega9c (25.5 %), 15 : 0 iso (18.7 %) and 17 : 0 iso (14.6 %), and the major hydroxy fatty acids were 11 : 0 iso 3-OH (5.0 %), 13 : 0 iso 3-OH (3.4 %) and 17 : 0 iso 3-OH (1.0 %). The major isoprenoid quinone was Q-8. The G+C content of the DNA of the type strain was 63.0 mol%. On the basis of the data from this study, strain R2A16-10(T) represents a novel species of the genus Dyella, for which the name Dyella yeojuensis sp. nov. is proposed. The type strain is R2A16-10(T) (=KACC 11405(T)=DSM 17673(T)).
International journal of systematic and evolutionary microbiology 10/2006; 56(Pt 9):2079-82. · 2.11 Impact Factor