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ABSTRACT: To study the effects of transforming growth factor-β1 (TGF-β1) on the gene expression of connective tissue growth factor (CTGF) in cultured lung fibroblasts of embryonic rats in vitro.
Wistar rats of embryonic 19 days were used for primary culture of lung fibroblasts (LFs). The cells in the experimental group were treated by different concentrations (1, 5 or 10 ng/mL) and different durations (12, 24 or 48 hrs) of TGF-β1 to stimulate the LFs. The cells in the control group were cultured in serum-free medium. RT-PCR method was applied to detect CTGF mRNA expression in LFs.
Compared with the control group, the levels of CTGF mRNA in LFs in the experimental group increased significantly (P<0.05). CTGF mRNA expression gradually increased with increasing concentration and duration of TGF-β1 treatment (P<0.05).
TGF-β1 can stimulate CTGF gene expression in LFs and increase CTGF gene expression in a dose-and time-dependent manner.
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 01/2011; 13(1):36-9.
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ABSTRACT: Recent study found psychosocial factors play some important roles in carcinogenesis and development of malignant tumors, but its mechanisms remain unclear. This study was to investigate the impact of chronic restraint stress on splenocyte immunity and growth of mouse forestomach carcinoma (MFC) xenografts in Kunming mice, and provide evidences for exploring the mechanisms of psychosocial factors function on malignant tumors.
A total of 60 Kunming mice were randomized into normal control group, restraint stress group, tumor-bearing group and tumor plus restraint stress group; each group contained 15 mice. Chronic restraint stress models were established in restraint stress group and tumor plus restraint stress group; MFC xenograft models were established in tumor-bearing group and tumor plus restraint stress group 4 weeks later. Mice were killed 10 days after inoculation of MFC cells. The weight of MFC xenografts were measured. The proliferation and cytotoxicity of splenocytes were detected by MTT assay. The level of interleukin-2 (IL-2) in splenocyte culture supernants was detected by enzyme-linked immunoabsorbent assay (ELISA).
The weight of MFC xenografts was (1.39+/-0.39) g in tumor-bearing group and (2.10+/-0.52) g in tumor plus restraint stress group; MFC xenografts grew faster in tumor plus restraint stress group than in tumor-bearing group (P<0.01), with a tumor growth rate of 51.08%. In normal control group, restraint stress group, tumor-bearing group, and tumor plus restraint stress group, the stimulus indexes (SI) of T lymphocytes were 1.77+/-0.22, 1.70+/-0.17, 1.69+/-0.18, and 1.22+/-0.15, respectively; the SI of B lymphocytes were 1.73+/-0.14, 1.65+/-0.17, 1.64+/-0.21, and 1.33+/-0.11, respectively; the inhibition rate of MFC cell proliferation were (23.01+/-4.76)%, (19.47+/-3.70)%, (16.81+/-3.68)%, and (7.14+/-5.00)%, respectively, when the effector/target ratio was 5:1 and (33.03+/-3.91)%, (28.34+/-4.58)%, (24.94+/-2.97)%, and (13.49+/-7.94)%, respectively, when the effector/target ratio was 10:1; the levels of IL-2 in splenocyte culture supernatants were (260.03+/-14.96) pg/mL, (239.78+/-10.93) pg/mL, (238.11+/-13.50) pg/mL, and (186.34+/-10.42) pg/mL, respectively. Both chronic restraint stress and MFC xenografts impaired the proliferation, cytotoxicity, and IL-2 secretion of splenocytes; the two factors showed interactive effect (P<0.01), but the effect of chronic restraint stress was much more obvious.
Chronic restraint stress may impair the immune function and promote the growth of MFC xenografts in mice.
Ai zheng = Aizheng = Chinese journal of cancer 05/2008; 27(5):471-5.
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ABSTRACT: Suppressor of cytokine signaling (SOCS) is a new family of proteins produced in cells. It may play an important role in classic negative feedback loop to regulate cytokine signal transduction. SOCS-1 was observed and confirmed firstly. Expression of SOCS-1 can inhibit cytokine signal transduction of some cytokines, such as IL-6, LIF, OSM, INF-gamma, GH, and so on, many immune responses are regulated by them in vivo. Abnormal expression of SOCS-1 is closely related to some human diseases. It plays an important role in the development of leukemia, rheumatoid arthritis, liver cirrhosis and liver cancer. In this review, the advances of research on the relationship between SOCS-1 and cytokine, and its correlation with some diseases were summarized.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology 05/2007; 15(2):437-40.
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ABSTRACT: In order to investigate the suppressor of cytokine signaling-1 (SOCS-1) expression in peripheral blood mononuclear cells (PBMNC) of patients with acute and chronic myeloid leukemia and analyze its clinical significance, RT-PCR method was used for detecting SOCS-1 mRNA expression in PBMNC of 50 newly diagnosed patients. The result showed that positive expressions of SOCS-1 were observed in 4 of 25 patients with AML (16.00%), in 11 of 25 patients with CML (44.00%) and none in 10 normal controls. The differences between patients with AML and normal controls, and between patients with CML and normal controls were statistically significant. In CML group, 2 out of 12 cases with non-progression (chronic phase), 9 of 13 cases with progression showed the positive expression, the difference between two subgroups was statistically significant. Those CML patients with SOCS-1 mRNA expression had poor response to IFN-alpha. When they transformed into accelerated phase, SOCS-1 mRNA expression was more persistently and frequently observed, and no response to IFN-alpha was observed. Most of them had very poor prognosis. It is concluded that the SOCS-1 mRNA can be detected in the PBMNC of the patients with acute and chronic myeloid leukemia. The SOCS-1 mRNA expression in the patients with CML is higher than that in patients with AML, and it is higher in accelerated phase and blast crisis significantly. This phenomenon is highly related to the reaction of IFN-alpha and prognosis.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology 09/2006; 14(4):677-80.
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ABSTRACT: In order to study whether plasma can affect the structure and function of red blood cells during their storage period, the differences of pH value, concentration of K(+), Na(+), osmotic fragility, plasma hemoglobin, AchE, ATP, 2.3-DPG, P50 in suspended RBC, washed RBC, and RBC with various plasma volume at different storage times were compared. The results showed that plasma helped the blood to keep the RBC at high pH value, low K(+), high Na(+) and maintain RBC-ATP, oxygen carry capacity and deformability, but no effect on maintenance of osmotic fragility, and levels of plasma hemoglobin, AchE, ATP and 2.3-DPG was found in preservated blood. In conclusion, human plasma may be in favour of the preservation of red blood cells.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology 09/2005; 13(4):692-7.
