[Show abstract][Hide abstract] ABSTRACT: This study aimed to investigate genetic features of CCV strains detected in Korea. The sequences of M gene obtained from 22 dogs with enteritis over the 5 year-period were evaluated. Sequence comparison study revealed 87.2 to 100% nucleotide homology among the 22 Korean CCV strains. Comparing with typical reference CCV strains (type II), the nucleotide sequence homology was ranged from 86.3% to 98.3% (89.1% to 99.2% in amino acid), while it was ranged from 87.7% to 97.8% (92.4% to 100% in amino acid) comparing with FCoV-like CCV strains (type I). Three amino acid variations in M gene were characteristic in Korean CCV strains. Phylogenetic study found that the 22 Korean CCV strains belonged to 4 typical CCV clusters (i.e. unique Korean CCV cluster, type II and TGEV cluster, intermediate cluster between type I and II, and type I cluster). This study was the first investigation identified genetic differences of M gene from Korean CCV strains and provided a platform for molecular identification of different Korean CCV strains.
[Show abstract][Hide abstract] ABSTRACT: The purpose of this study was to genetically characterize CPV isolates from Korea. The VP2 gene of 31 isolates was characterized by DNA sequencing and their phylogeny. Among the 31 field CPV isolates, 28 isolates were classified as type 2a and other 3 isolates as type 2b. The isolates in 2a-I, II and III subclusters have unique mutations. The isolates in 2a-IV and V subclusters had similar amino acid sequences to type 2a isolates from other parts of the world. The isolates in type 2b had similar amino acid sequences to type 2b isolates from Asia, Italy, and U.S.A. The molecular analysis of VP2 gene of CPV provided the useful information for the identification of CPV types and the understanding of their genetic relationship.
Journal of Veterinary Medical Science 08/2008; 70(7):719-22. DOI:10.1292/jvms.70.719 · 0.88 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The clinical utility of various specimens was examined for the early diagnosis of canine distemper (CD). Seven healthy dogs at 17 weeks of age were experimentally infected with a field isolate of canine distemper virus. The RT-PCR was carried out to detect CDV NP gene. Dogs showed mild fever and leukopenia, however, typical clinical signs of CD were not seen through the experimental period. CDV amplicons were detected more, earlier and for longer period in the conjunctival swabs than in the other samples employed. These results suggested that conjunctival swab samples, which are easy to obtain and non-invasive, would be the most suitable and practical specimen for the early antemortem diagnosis of CDV infection.
Journal of Veterinary Medical Science 09/2006; 68(8):877-9. DOI:10.1292/jvms.68.877 · 0.88 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: To draw inferences about the sensitivity and specificity of the newly developed ELISA test for bovine paratuberculosis (PTB) diagnosis and posterior distribution on the prevalence of PTB in a province of Korea, we applied Bayesian approach with Gibbs sampler to the data extracted from the prevalence study in 1999. The data were from a single test results without a designated gold test. The prevalence estimates for PTB in study population ranged 3.2-5.3% for conservative and 6.7-7.1% for liberal, depending on the priors used. The simulated specificities of the ELISA close to one another, ranging 84.7-90.6%, whereas the sensitivity was somewhat spread out depending largely on the priors with a range of 46.4-88.2%. Our findings indicate that the ELISA method appeared useful as a screening tool at a minimum level in comparison to other diagnostic tests available for this disease in terms of sensitivity. However, this advantage comes at a cost of having low specificity of the test.
Journal of Veterinary Science 05/2003; 4(1):51-6. · 1.14 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study was conducted to evaluate the antibody levels of alpha-toxin, capsular polysaccharides (CPS) and fibronectin-binding protein (FnBP) in rabbits immunized with an experimental vaccine against Staphylococcus aureus and to develop the bovine mastitis subunit vaccine in the future. Enzyme immunoassay was used for detection of IgG antibodies against staphylococcal CPS, alpha-toxin and FnBP. The levels of specific antibodies against CPS, alpha-toxin and FnBP in immunized rabbits were significantly increased after first immunization compared with control animals (p<0.05). Of three antigen used in vaccine, immunogenicity of CPS was relatively lower, compared with those of alpha toxin and fibronectin binding protein. Numbers of S. aureus in blood of immunized groups were lower than those of control group after bacterial challenge. But the bacterial numbers among immunized groups were not significantly different. S. aureus counts in excised organs were significantly lower in all immunized rabbits than in PBS-control group (p<0.05). The present study showed that alpha-toxin, capsular polysaccharide and fibronectin binding protein included in a subunit vaccine were protective.
Journal of Veterinary Medical Science 10/1999; 61(9):995-1000. DOI:10.1292/jvms.61.995 · 0.88 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We evaluated the patterns of serology and fecal viral shedding for any differences by hemagglutination inhibition (HI) and real-time PCR on Korean CPV-2 isolates (CPV-2a-I, CPV-2a-V and CPV-2b). We successfully detected fecal viral shedding from samples extracted 2-3 d.p.i., regardless of the onset of clinical signs. In addition, the pattern of viral shedding differed depending on the CPV-2 isolates used for inoculation. We also observed differences in the serological pattern that was also depended on the CPV-2 isolates inoculated. The onset and amount of fecal viral shedding were not correlated with the level of antibody titers in this study. Our study is a valuable resource for understanding the different pathobiology of the CPV-2 isolates and the correlation between the patterns of serum antibody titer and fecal viral shedding.