Chen-Yang Huang

Chinese Academy of Agricultural Sciences, Peping, Beijing, China

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Publications (4)3.03 Total impact

  • Wei-Wei Kong · Chen-Yang Huang · Qiang Chen · Ya-Jie Zou · Meng-Ran Zhao · Jin-Xia Zhang ·
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    ABSTRACT: Little is known about the mechanism of how trehalose responds to various abiotic stresses although trehalose is considered as an important protectant in fungi. We investigated the role of nitric oxide (NO) in regulating trehalose accumulation during heat stress in Pleurotus eryngii var. tuoliensis. The addition of 100 or 200 g trehalose/l significantly inhibited the production of thiobarbituric acid-reactive substance under heat stress in mycelial cells. High temperature induced endogenous trehalose accumulation and sodium nitroprusside, a NO donor, further enhanced trehalose accumulation. Finally, heat-induced trehalose accumulation could be arrested by the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-1-oxyl-3-oxide, at 250 μM by inhibiting the transcription of trehalose phosphate synthase gene. Thus NO plays an important role in the regulation of trehalose accumulation during abiotic stresses in P. eryngii var. tuoliensis.
    Biotechnology Letters 07/2012; DOI:10.1007/s10529-012-0988-2 · 1.59 Impact Factor
  • Ya-Jie Zou · He-Xiang Wang · Tzi-Bun Ng · Chen-Yang Huang · Jin-Xia Zhang ·
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    ABSTRACT: A novel laccase from the edible mushroom Hericium coralloides was purified by ion exchange chromatography on diethylaminoethyl (DEAE) cellulose, carboxymethyl (CM) cellulose, and Q-Sepharose columns followed by fast protein liquid chromatography gel filtration on a Superdex 75 column. Analysis by gel filtration and SDS-PAGE indicated that the protein is a monomer in solution with a molecular mass of 65 kDa. Its N-terminal amino acid sequence was AVGDDTPQLY, which exhibits partial sequence homology to previously isolated laccases. Optimum activity was observed at pH 2.2 and at 40°C. The enzyme showed activity toward a variety of substrates, the most sensitive of which was 2,2'-azinobis [3-ethylbenzothiazolone-6-sulfonic acid] diammonium salt (ABTS). The degradation activity toward substrates was ABTS > N,N-dimethyl-1,4-phenylenediamine > catechol > 2-methylcatechol > pyrogallol. The laccase did not exert any antiproliferative activity against Hep G2 or MCF 7 tumor cell lines at a concentration of 60 μM, unlike some previously reported mushroom proteins, but showed significant activity toward human immunodeficiency virus-1 (HIV-1) reverse transcriptase with an IC(50) of 0.06 μM.
    The Journal of Microbiology 02/2012; 50(1):72-8. DOI:10.1007/s12275-012-1372-6 · 1.44 Impact Factor
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    Jin-Xia ZHANG · Chen-Yang HUANG · Jiang CHEN · Wang-Qiu DENG · Tai-Hui LI · Wei GAO ·
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    ABSTRACT: A wild A-Wei-Mo specimen collected from Xinjiang was identificated with morphological methods. The results showed that it was consistent with the morphological characteristics of Pleurotus eryngii var. tuoliensis, but different from those of Pleurotus nebrodensis reported in Europe. In addition, the strain CCMSSC 02514 isolated from the wild mushroom specimen was analyzed with molecular menthods. Based on rDNA ITS sequences from the phylogenetic tree, the relationship among Bai-Ling-Gu in China, Pleurotus nebrodensis and Pleurotus eryngii var. ferulae in Italy, and Pleurotus eryngii are analyzed. The rDNA ITS sequences of CCMSSC 02514 and Pleurotus eryngii var. tuoliensis AFRL 6022 are identical. Combined with morphology and rDNA ITS sequence analysis, we considered that Bai-Ling-Gu in China is different from the European Pleurotus nebrodensis, and the former is a branch of Pleurotus eryngii evolved independently in China. The scientific name for the Chinese Bai-Ling-Gu in China should be Pleurotus eryngii var. tuoliensis.
    01/2011; 12(5).
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    Jin-Xia ZHANG · Chen-Yang HUANG · Jiang CHEN · Wei GAO · Su-Yue ZHENG ·
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    ABSTRACT: There are rich in wild germplasm resources of edible mushrooms and cultivable 93 species arise in China, which belong to 33 genera. Among them, 70 species were domesticated successfully and 50 species were cultivated in various scale; 33 species were produced commercially, which belong to 5 orders, 12 families, 18 genera. Biosynthesis of edible mushrooms is different from autotrophic green plants which synthesize organic compounds by photosynthesis. And they are heterotrophic organisms which break down the photosynthate of plants and construct themselves by bodies absorption of nourishment penetration. This biosynthesis property results in difference of evaluation methods from green plants for wild edible mushroom germplasm. Compared with green plants, diversities of their fruiting bodies are simple in morphology and they are prone to be changed with influenced of environment conditions. It often results in confusion for the taxonomy identification based on morphology. On the other hand, Taxonomy to species is more difficult based on morphology in cultivable mushrooms which have multiple relative species in the same genus, for example, Pleurotus, Auricularia and Armillaria. Therefore, ITS sequencing generally was used to identify biological species for the sample and isolates in the wide germplasm evaluation of cultivable mushrooms. RAPD(Random Amplified Polymorphic DNA), ISSR(Inter-Simple Sequence Repeats) and ITSInternal Transcribed Spacerssequencing were used as routine tools to identify isolates genuineness because the isolates are usually contaminated by other fungi similar in colony character. The spores spreading with air flow makes mushrooms to distribute in widely geographical region, and it further generates differences among the individuals or population in one species. The difference of available cultivation characters is formed in the long-term evolution with various climate and ecological conditions. Cultivation characters are evaluated in the fruiting test, which are related in growth and temperature reaction for mycelia, fruiting character, yield, resistance, commodity shape and endurance for transportation. In order to take the distant advantages, the genetic distance will be analyzed for the germplasm materials compared with cultivated strains based on the test of biochemical or molecular biology.
    01/2010; 11(2).