Wendy Tai

Western University of Health Sciences, Pomona, California, United States

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Publications (2)5.57 Total impact

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    ABSTRACT: The Mycobacterium tuberculosis gene lipF, Rv3487c, is transcriptionally upregulated by exposure to acidic growth media. We previously identified a 477 base pair (bp) region of DNA 147 bp upstream of lipF that is transcriptionally upregulated by exposure to growth media at pH 4.5 [Saviola, B., Woolwine, S., Bishai, W. R., 2003. Isolation of acid-inducible genes of Mycobacterium tuberculosis with the use of recombinase-based in vivo expression technology. Infect. Immun. 71, 1379-1388]. In this study we truncate the lipF promoter region first from the 3' DNA end and then from the 5' DNA end. The truncated promoter regions were placed upstream of the gene for the green fluorescent protein (gfp) and each promoter region was analyzed in Mycobacterium smegmatis for its ability to undergo transcriptional upregulation in response to acid stress. A minimal acid-inducible promoter region was identified and is located between -515 bp and -573 bp with respect to the start site of translation of lipF. The 59 bp minimal promoter region is a defined DNA sequence that confers full promoter activity that is transcriptionally upregulated in response to acid stress. Primer extension analysis was performed on acid-induced M. smegmatis bearing the minimal promoter region fused to gfp and revealed a start site of transcription specifically upregulated by acid stress corresponding to -511 bp upstream of lipF with respect to the start of translation.
    Gene 07/2007; 395(1-2):22-8. DOI:10.1016/j.gene.2006.12.037 · 2.08 Impact Factor
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    ABSTRACT: The recent emergence of multiple avian influenza A subtypes that cause human disease (i.e., H5N1, H9N2 and H7N7), coupled with the fear that one of these strains might precipitate a new pandemic, underscores the need to develop new technological approaches to immunization which elicit protective immune responses against multiple subtypes of influenza A. In response to this demand, several matrix 2 protein ectodomain segments (M2eA) corresponding to the H1N1, H5N1 and H9N2 influenza strains were formulated using a novel liposome-based vaccine technology and evaluated as potential immunogens for developing a "universal" influenza vaccine. Mice immunized with liposomal M2eA survived homologous challenges with H1N1 (100% survival) or H9N2 (80% survival) influenza strains. There were significant reductions in their lung viral load as well as in immunized mice challenged with the H5N1 subtype. The mice vaccinated with an M2eA segment corresponding to the H1N1 and H6N2 (a reassortant influenza A virus carrying the M2eA from PR8/34) strains elicited elevated IgG ELISA antibody titers to this M2eA epitope segment and antiserum from these immunized mice provided passive protection (100% survival) to naïve mice receiving a lethal dose of H6N2 influenza virus. These results provide the first evidence that recombinant M2eA epitopes to multiple subtypes elicited immune protection against a homologous challenge and provides further evidence in favor of the development of a "universal" influenza vaccine based on M2eA.
    Vaccine 07/2006; 24(24):5158-68. DOI:10.1016/j.vaccine.2006.04.008 · 3.49 Impact Factor

Publication Stats

102 Citations
5.57 Total Impact Points

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Institutions

  • 2007
    • Western University of Health Sciences
      • Department of Basic Medical Sciences
      Pomona, California, United States
  • 2006
    • California State Polytechnic University, Pomona
      Pomona, California, United States