Linda Giudice

Publications (2)8.6 Total impact

• Article: High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples.

  Richard E Leach, Philip Jessmon, Christos Coutifaris, Michael Kruger, Evan R Myers, Rouba Ali-Fehmi, Sandra A Carson, Richard S Legro, William D Schlaff, Bruce R Carr, Michael P Steinkampf, Susan Silva, Phyllis C Leppert, Linda Giudice, Michael P Diamond, D Randall Armant
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  ABSTRACT: Although histological dating of endometrial biopsies provides little help for prediction or diagnosis of infertility, analysis of individual endometrial proteins, proteomic profiling and transcriptome analysis have suggested several biomarkers with altered expression arising from intrinsic abnormalities, inadequate stimulation by or in response to gonadal steroids or altered function due to systemic disorders. The objective of this study was to delineate the developmental dynamics of potentially important proteins in the secretory phase of the menstrual cycle, utilizing a collection of endometrial biopsies from women of fertile (n = 89) and infertile (n = 89) couples. Progesterone receptor-B (PGR-B), leukemia inhibitory factor, glycodelin/progestagen-associated endometrial protein (PAEP), homeobox A10, heparin-binding EGF-like growth factor, calcitonin and chemokine ligand 14 (CXCL14) were measured using a high-throughput, quantitative immunohistochemical method. Significant cyclic and tissue-specific regulation was documented for each protein, as well as their dysregulation in women of infertile couples. Infertile patients demonstrated a delay early in the secretory phase in the decline of PGR-B (P < 0.05) and premature mid-secretory increases in PAEP (P < 0.05) and CXCL14 (P < 0.05), suggesting that the implantation interval could be closing early. Correlation analysis identified potential interactions among certain proteins that were disrupted by infertility. This approach overcomes the limitations of a small sample number. Protein expression and localization provided important insights into the potential roles of these proteins in normal and pathological development of the endometrium that is not attainable from transcriptome analysis, establishing a basis for biomarker, diagnostic and targeted drug development for women with infertility.
  Human Reproduction 01/2012; 27(3):814-28. · 4.47 Impact Factor
• Article: A twenty-first century research agenda for polycystic ovary syndrome.

  Richard S Legro, Ricardo Azziz, Linda Giudice
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  ABSTRACT: Notwithstanding the significant progress made in our understanding of polycystic ovary syndrome (PCOS), the field remains ripe for further discovery and more intensive translational research. Immediate priorities include developing evidence-based criteria for diagnosing PCOS and for assessing the response to the various treatments available. The basis for the identification of PCOS remains mainly expert opinion, and the lack of universally accepted evidence-based criteria limits the generalizability of research studies on PCOS. Additional important areas requiring intensive investigation include the natural history and etiology and the long-term sequelae and prevention of the disorder. Overall, this disorder is a prototype for the benefits of translational science and a transdisciplinary approach to understanding the pathophysiology and therapy for anovulatory infertility.
  Bailli&egrave re s Best Practice and Research in Clinical Endocrinology and Metabolism 07/2006; 20(2):331-6. · 4.12 Impact Factor