Carine Robert

Publications (9)50.25 Total impact

• Article: Supported neodymium catalysts for isoprene and rac-β-butyrolactone polymerization: modulation of reactivity by controlled grafting.

  Michael Terrier, Emilie Brulé, Marta J Vitorino, Noureddine Ajellal, Carine Robert, Régis M Gauvin, Christophe M Thomas
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  ABSTRACT: A series of hybrid materials, bearing neodymium silylamide initiating groups, have been shown to mediate isoprene polymerization when combined with alkyl aluminum activators [methylaluminoxane, AlEt(2)Cl, Al(iBu)(3)]. The surface species nature and relative distribution were correlated with isoprene polymerization activity and selectivity. This approach to stereocontrol modulation has been extended to racemic β-butyrolactone isoselective ring opening polymerization.
  Macromolecular Rapid Communications 01/2011; 32(2):215-9. · 4.60 Impact Factor
• Source

  Available from: Christophe M. Thomas

  Article: Tandem synthesis of alternating polyesters from renewable resources.

  Carine Robert, Frédéric de Montigny, Christophe M Thomas
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  ABSTRACT: The vast majority of commodity materials are obtained from petrochemical feedstocks. These resources will plausibly be depleted within the next 100 years, and the peak in global oil production is estimated to occur within the next few decades. In this regard, biomass represents an abundant carbon-neutral renewable resource for the production of polymers. Here we report a new strategy, based on tandem catalysis, to obtain renewable materials. Commercially available complexes are found to be efficient catalysts for alternating polyesters from the cyclization of dicarboxylic acids followed by alternating copolymerization of the resulting anhydrides with epoxides. This operationally simple method is an attractive strategy for the production of new biodegradable polyesters.
  Nature Communications 01/2011; 2:586. · 7.40 Impact Factor
• Article: Suzuki-Miyaura coupling of NHC-boranes: a new addition to the C-C coupling toolbox.

  Julien Monot, Malika Makhlouf Brahmi, Shau-Hua Ueng, Carine Robert, Marine Desage-El Murr, Dennis P Curran, Max Malacria, Louis Fensterbank, Emmanuel Lacôte
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  ABSTRACT: Complexes of triaryl- and trialkylboranes with N-heterocyclic carbenes (NHCs) participate in Suzuki-Miyaura cross-coupling reactions and provide coupled products in good yields under base-free conditions. The reaction can be applied to Csp(2)-Csp(2) and Csp(2)-Csp(3) carbon-carbon bond formation with triflates, iodides, bromides, and chlorides. These results enrich the utility of NHC-borane complexes, which can be added to the toolkit of Suzuki-Miyaura cross-couplings, along with boronic acids and organotrifluoroborates.
  Organic Letters 10/2009; 11(21):4914-7. · 5.86 Impact Factor
• Article: Matching the chirality of monodentate N-heterocyclic carbene ligands: a case study on well-defined palladium complexes for the asymmetric alpha-arylation of amides.

  Xinjun Luan, Ronaldo Mariz, Carine Robert, Michele Gatti, Sascha Blumentritt, Anthony Linden, Reto Dorta
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  ABSTRACT: N-Heterocyclic carbene ligands derived from C(2)-symmetric diamines with naphthyl side chains are introduced as chiral monodentate ligands, and their palladium complexes (NHC)Pd(cin)Cl are prepared. These compounds exist as a mixture of diastereomers, and the palladium complexes can be successfully separated and their absolute stereochemistry assigned. When used in the asymmetric intramolecular alpha-arylation of amides, oxindoles with quaternary carbon centers can be obtained in high yield and selectivity when correctly matching the chirality of the NHC complexes.
  Organic Letters 12/2008; 10(24):5569-72. · 5.86 Impact Factor
• Article: All-trans-retinoic acid enhances apoptosis induction by tyrosine kinase inhibitors in the eosinophilic leukemia-derived EoL-1 cell line.

  Carine Robert, Agota Apàti, Christine Chomienne, Béla Papp
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  ABSTRACT: Imatinib and retinoids induce apoptosis in FIP1L1/PDGFRalpha-positive EoL-1 leukemia cells. Although imatinib induces complete remission in most FIP1L1/PDGFRalpha-positive patients, response to imatinib is sometimes suboptimal. In order to enhance the potency of the molecularly targeted therapy of eosinophilic leukemia, we investigated the effect of retinoids combined with tyrosine kinase inhibitors on EoL-1 cells. We demonstrate that retinoids combined with tyrosine kinase inhibitors lead to enhanced apoptosis induction in EoL-1 cells. Our results suggest that tyrosine kinase inhibitors combined with retinoids may constitute a valuable therapeutic approach for sensitive neoplasias that may display enhanced anti-leukemic potency when compared to single drug treatments.
  Leukemia Research 03/2008; 32(2):343-6. · 2.92 Impact Factor
• Article: Generation of phosphorus-centered radicals via homolytic substitution at sulfur.

  Paola Carta, Nicolas Puljic, Carine Robert, Anne-Lise Dhimane, Louis Fensterbank, Emmanuel Lacôte, Max Malacria
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  ABSTRACT: A novel radical domino process relying on the homolytic cleavage of P-S bonds allows the preparation of phosphorus-containing molecules through addition of P-centered radicals onto olefins. The key step of this reaction is a homolytic substitution on a sulfur atom. The scope of the reaction is broad. Diaminophosphonyl radicals whose reactivity was unknown react smoothly with olefins. Use of tin hydride can be avoided. A radical thiophosphinoylation of triple bonds has been uncovered. [reaction: see text]
  Organic Letters 04/2007; 9(6):1061-3. · 5.86 Impact Factor
• Article: Frequent antibody production against RARalpha in both APL mice and patients.

  Marie Robin, Juliette Andreu-Gallien, Marie-Helene Schlageter, Djaouida Bengoufa, Isabelle Guillemot, Katerina Pokorna, Carine Robert, Jerome Larghero, Philippe Rousselot, Emmanuel Raffoux, Herve Dombret, Pierre Fenaux, Marika Pla, Dominique Charron, Rose-Ann Padua, Christine Chomienne
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  ABSTRACT: In an acute promyelocytic leukemia (APL)-transplantable mouse model, we previously reported the presence of antibodies recognizing PML-RARalpha and RARalpha in the sera of ATRA-treated mice. To evaluate this immune response, we determined the prevalence of anti-RARalpha antibodies in a cohort of 48 APL mice, treated by ATRA (n = 24) or by placebo pellets (n = 24), and in a preliminary subset of 9 patients with APL using a specific enzyme-linked immunosorbent assay (ELISA). In APL mice, significantly higher antibody levels were observed at the latest time points (day 48 to 58 levels superior to day 15 to 18 or day 28 to 38 levels). Antibody levels were higher in ATRA-treated mice than in placebo-treated mice and were also predictive of better survival. In the patients with APL, anti-RARalpha antibodies were detected at diagnosis and after maintenance therapy, reminiscent of the ATRA-treated APL mice. Antinuclear or antineutrophil cytoplasmic autoantibodies were also detected. These data reveal for the first time that in patients with APL an immune response may be detected at diagnosis and enhanced after maintenance therapy.
  Blood 10/2006; 108(6):1972-4. · 9.90 Impact Factor
• Article: Apoptosis induction by retinoids in eosinophilic leukemia cells: implication of retinoic acid receptor-alpha signaling in all-trans-retinoic acid hypersensitivity.

  Carine Robert, Laurent Delva, Nicole Balitrand, Sarolta Nahajevszky, Tamàs Masszi, Christine Chomienne, Béla Papp
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  ABSTRACT: Hypereosinophilic syndrome (HES) has recently been recognized as a clonal leukemic lesion, which is due to a specific oncogenic event that generates hyperactive platelet-derived growth factor receptor-alpha-derived tyrosine kinase fusion proteins. In the present work, the effect of retinoids on the leukemic hypereosinophilia-derived EoL-1 cell line and on primary HES-derived cells has been investigated. We show that all-trans-retinoic acid (ATRA) inhibits eosinophil colony formation of HES-derived bone marrow cells and is a powerful inducer of apoptosis of the EoL-1 cell line. Apoptosis was shown in the nanomolar concentration range by phosphatidylserine externalization, proapoptotic shift of the Bcl-2/Bak ratio, drop in mitochondrial membrane potential, activation of caspases, and cellular morphology. Unlike in other ATRA-sensitive myeloid leukemia models, apoptosis was rapid and was not preceded by terminal cell differentiation. Use of isoform-selective synthetic retinoids indicated that retinoic acid receptor-alpha-dependent signaling is sufficient to induce apoptosis of EoL-1 cells. Our work shows that the scope of ATRA-induced apoptosis of malignancies may be wider within the myeloid lineage than thought previously, that the EoL-1 cell line constitutes a new and unique model for the study of ATRA-induced cell death, and that ATRA may have potential for the management of clonal HES.
  Cancer Research 07/2006; 66(12):6336-44. · 7.86 Impact Factor
• Article: Frequent antibody production against RAR in both APL mice and patients

  Marie Robin, Juliette Andreu-Gallien, Marie-Helene Schlageter, Djaouida Bengoufa, Isabelle Guillemot, Katerina Pokorna, Carine Robert, Jerome Larghero, Philippe Rousselot, Emmanuel Raffoux, Herve Dombret, Pierre Fenaux
  [show abstract] [hide abstract]
  ABSTRACT: In an acute promyelocytic leukemia (APL)- transplantable mouse model, we previously reported the presence of antibodies rec- ognizing PML-RAR and RAR in the sera of ATRA-treated mice. To evaluate this immune response, we determined the prevalence of anti-RAR antibodies in a cohort of 48 APL mice, treated by ATRA (n 24) or by placebo pellets (n 24), and in a preliminary subset of 9 patients with APL using a specific enzyme-linked immunosorbent assay (ELISA). In APL mice, significantly higher antibody levels were observed at the latest time points (day 48 to 58 levels superior to day 15 to 18 or day 28 to 38 levels). Antibody levels were higher in ATRA-treated mice than in placebo-treated mice and were also pre- dictive of better survival. In the patients with APL, anti-RAR antibodies were de- tected at diagnosis and after mainte- nance therapy, reminiscent of the ATRA- treated APL mice. Antinuclear or antineutrophil cytoplasmic autoantibod- ies were also detected. These data reveal for the first time that in patients with APL an immune response may be de- tected at diagnosis and enhanced after maintenance therapy. (Blood. 2006;108: 1972-1974)