Lihong Fan

Hubei University of Science and Technology, Hu-pei-ts’un, Shanxi Sheng, China

Are you Lihong Fan?

Claim your profile

Publications (42)66.16 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Tissue repair is a spontaneous process which initiated on wounding. If this complex mechanism is disturbed or impaired, the use of biomaterials might increase the chance of successful healing. In this view, a water soluble chitosan derivative, quaternized-carboxymethyl chitosan (QCMC) was prepared and collagen peptides (COP) were grafted to the backbone by carbodiimide method. The reaction conditions affected the degree of substitution (DS) were studied including the mass ratio of collagen peptide to QCMC, reaction temperature and reaction time. The hydrogen peroxide scavenging activity could be different by change the DS, concentration and molecular weight. MTT assay was used to investigate the cell viability of the derivative. The results indicated that the introduction of collagen peptide into the QCMC improved its hydrogen peroxide scavenging activity and cell viability with the DS and concentration increased. Therefore, QCMC conjugated with collagen peptides may prove beneficial to the process of the wound-healing.
    International journal of biological macromolecules. 07/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Collagen grafted chitosan was prepared with microbial transglutaminase (MTGase) as biocatalyst which showed high efficiency, selectivity, mild reaction condition and environmental friendliness. The reaction conditions that influenced the degree of substitution (DS) were optimized, which included the reaction time, the reaction temperature, the mass ratio of collagen to chitosan and the mass ratio of MTGase to chitosan. In this study, the water-solubility collagen–chitosan could serve not only to reduce the loss of moisture but also to absorb the moisture. And the moisture absorption and moisture retention abilities were closely related to the DS values. In addition, in vitro antioxidant activity was evaluated in terms of DS values and concentration. Furthermore, L929 mouse fibroblasts were cultured with collagen–chitosan, and methylthiazol tetrazolium (MTT) assay exhibited that collagen–chitosan with DS of 0.660 displayed pronounced cell viability at 2.5 mg/ml. Therefore, the water-soluble collagen–chitosan showed the potentiality to repair skin in cosmetic, biomedical and pharmaceutical fields.
    Carbohydrate Polymers 05/2014; 105:253–259. · 3.48 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Collagen peptide grafted carboxymethylated chitosan was synthesized by using microbial transglutaminase as bio-catalyst. The catalyzed reaction exhibited high efficiency, selectivity, environmental friendliness. Collagen peptide grafted carboxymethylated chitosan was characterized by Fourier transform infrared spectroscopy. The process conditions were optimized including the reaction time, the reaction temperature, the molar ratio of collagen peptide to carboxymethylated chitosan and the pH value. The different molecular weight, concentration and degree of substitution of collagen peptide-carboxymethylated chitosan had crucial effects on the hydrogen peroxide scavenging activity of collagen peptide-carboxymethylated chitosan. The methyl thiazolyl tetrazolium assay showed that at a suitable concentration collagen peptide-carboxymethylated chitosan with different degree of substitution value could promote L929 mouse fibroblasts effectively. Therefore, the results suggest that collagen peptide-carboxymethylated chitosan could be potential wound dressings for clinical applications.
    Reactive and Functional Polymers 03/2014; · 2.51 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Tissue engineering is aiming to build an artificial environment or biological scaffold material that imitates the living environment of cells in the body. In this work, carboxymethyl cellulose sulfates were prepared by reacting carboxymethyl cellulose with (N(SO3Na)3) which was synthesized by sodium bisulfite and sodium nitrite in aqueous solution. The reaction conditions affected the degree of substitution (DS) were measured by the barium sulfate nephelometry method. And the anticoagulant activity of carboxymethyl cellulose sulfates with different DS, concentration and molecular weights were investigated by the activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT). In addition, the effect of carboxymethyl cellulose sulfates on wound healing had been evaluated by the rate of wound healing and the histological examinations. The results indicated that the introduction of sulfate groups into the carboxymethyl cellulose sulfates improved its anticoagulant activity, and the wound dressings treated with carboxymethyl cellulose sulfates obviously promoted wound healing.
    International journal of biological macromolecules 02/2014; · 2.37 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hydroxyethyl cellulose sulfate (HECS) was synthesized by sulfation of hydroxyethyl cellulose with N(SO3Na)3, which was manufactured by reaction of sodium bisulfite and sodium nitrite. Barium sulfate nephelometry and light scattering method were used to determine degrees of substitution (DSs) and molecular weights (Mws), respectively. Sulfate products with DS values from 0.26 to 1.88 and Mw in the range of 12.1–54.8 kDa were obtained at temperatures from 30°C to 80°C. Furthermore, the anticoagulant activity of HECS with different DSs, concentrations, and Mws was studied. Clotting assays revealed that the introduction of sulfate groups into hydroxyethyl cellulose could improve its anticoagulant activity.
    Journal of Carbohydrate Chemistry 01/2014; 33(4). · 0.85 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Carboxymethyl cellulose used in wounds has little antioxidant capacity. The aim of the present study was to improve the scavenging ability of carboxymethyl cellulose by modified with collagen peptide. The reaction conditions have been optimized by varying mass ratio of collagen peptide to carboxymethyl cellulose, temperature and reaction time. Antioxidant activities of carboxymethyl cellulose derivatives (CMCC) were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide radicals and the reducing power. The effects of concentration, degree of substitution (DS) and molecular weight on three different radicals scavenging activity and reducing power were examined. Methylthiazol tetrazolium (MTT) assay was used to evaluate the fibroblasts cells cytotoxicity of CMCC. Results showed that the scavenging effects of CMCC increased with the increasing of DS and concentration. This product of CMCC possesses a distinct antioxidant capacity on radicals.
    Carbohydrate Polymers 01/2014; 112:32–38. · 3.48 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Oxidized carboxymethyl cellulose (OCMC) was prepared by an oxidation reaction of carboxymethyl cellulose in the presence of sodium periodate. In situ crosslinked hydrogels were obtained through the crosslinking reaction between the active aldehyde of OCMC and the amino groups of the carboxymethyl chitosan (CMCS). The structure of the hydrogels was characterized by FTIR and scanning electron microscopy. Gelation time test showed that the hydrogel had the shortest gelation time of 24 s. The equilibrium fluid content, which represented the swelling degree, was evaluated and we found that the pH increased from 3.0 to 9.0, the equilibrium fluid content increased, and the highest equilibrium fluid content reached 312.83% as pH = 9.0. The wound healing efficacy of the hydrogel was evaluated in experimental deep second degree burns using a rat model. Results indicated that the wound covered with hydrogel was completely filled with new epithelium within 2 weeks, without any significant adverse reactions. The in situ crosslinked hydrogel fulfilled many critical elements in a wound dressing material. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
    Journal of Applied Polymer Science 06/2013; 128(5). · 1.40 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Carboxymethyl κ-carrageenan (CMκC)/alginate (AL) blend fibers were prepared by spinning their mixture solution through a viscose-type spinneret into a coagulating bath containing aqueous CaCl2 and ethanol. The structure and properties of blend fibers were studied with the aid of infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis. The analyses indicated a good miscibility between AL and CMκC because of the strong interaction of the intermolecular hydrogen bonds. The mechanical properties and water-retention properties were also measured. The best values of tensile strength and breaking elongation were obtained when CMκC content was 30 wt.%. The water-retention properties and thermostability improved by blending method. Antibacterial fibers, obtained by the treatment of the fibers with an aqueous solution of silver nitrate, exhibited good antibacterial activity to Staphylococcus aureus.
    Journal of Biomaterials Science Polymer Edition 05/2013; · 1.70 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Collagen peptides grafted sodium alginate (SA-COP) was prepared by reacting alginate with collagen peptide via amide linkage in presence of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy sulfosuccinimide (NHS). The reaction conditions affected the degree of substitution (DS) were studied including the mass ratio of collagen peptide to sodium alginate, reaction temperature and reaction time. The hydrogen peroxide scavenging activity could be controlled by adjusting the DS, concentration and molecular weight. MTT assay was used to investigate the cell viability of SA-COP. The results indicated that the SA-COP exhibited better cell viability, and with the DS and concentration increasing of SA-COP, cell viability increased. The improved functionalities of the derivative might be explained by peptides characteristics.
    Carbohydrate polymers. 04/2013; 93(2):380-5.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To develop a renewable and compatible anticoagulant as potential heparin alternative, carboxymethyl starch sulfate (CMSS) was prepared by the reaction of carboxymethyl starch (CMS) and sulfating reagent [N(SO3Na)3]. The chemical structures of CMS and CMSS were characterized by Fourier transform infrared spectroscopy and 13C nuclear magnetic resonance. The influences of reaction parameters, including the pH of sulfating reagent, the molar ratio of sulfating reagent to CMS, reaction time, and temperature on the degree of substitution of sulfate groups (DS) of CMSS were studied. Meantime, the DS of each CMSS was determined by barium sulfate–glutin nephelometery method. Moreover, the anticoagulant activity of CMSS was investigated by the coagulation assays of activated partial thromboplastin time, thrombin time, and prothrombin time. The results revealed that the anticoagulant activity of CMSS was closely related to the DS value and concentration. The anticoagulant activity was promoted with the increasing of the DS and concentration. The molecular weight (Mw) in measured range had little impact on anticoagulant activity in contract to the DS and concentration. In this article, the CMSS with the DS of 1.91, concentration of 75 μg/mL and the Mw of 2.61 × 104 had the best blood anticoagulant activities. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
    Journal of Applied Polymer Science 01/2013; 127(6). · 1.40 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Carboxymethyl chitosan (CMC) was chemically modified by sulfating agent N(SO(3)Na)(3). The product carboxymethyl chitosan sulfate (CMCS) was characterized by Fourier transform infrared spectroscopy (FT-IR) and carbon-13 nuclear magnetic resonance spectroscopy ((13)C NMR). And the optimal reaction conditions were also studied by means of single factor experiment. MTT method was applied to evaluate the effects of CMCS on proliferation of skin fibroblasts. The results revealed the CMCS with sulfate content of 26.26% at the concentration of 100μg/ml best promoted the proliferation of skin fibroblasts.
    International journal of biological macromolecules 12/2012; · 2.37 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In order to develop a promising substitute for heparin, N-succinyl chitosan (NSC) was chemically modified by sulfating agent N(SO(3)Na)(3), which were synthesized with sodium bisulfite and sodium nitrite in aqueous solution. The N-succinyl chitosan sulfates (NSCS) products were characterized by infrared spectroscopy (FT-IR) and (13)C NMR. The degree of substitution (DS) of NSCS depended on the ratio of sulfating agent to N-succinyl chitosan, reaction temperature, reaction time and pH of sulfation agent. N-succinyl chitosan sulfates with DS of 1.97 were obtained under optimal conditions. The in vitro coagulation assay of NSCS was determined by activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) assays. The results showed that NSCS obviously prolonged APTT. The anticoagulant activity strongly depended on DS, molecular weight (M(w)) and concentration of NSCS. The anticoagulant activity of NSCS promoted with the increase of DS and concentration, and NSCS exhibited the best anticoagulant activity with the M(w) of 1.37×10(4).
    International journal of biological macromolecules 08/2012; 51(5):808-14. · 2.37 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hydroxypropyl chitosan (HPCS) was synthesized from chitosan and propylene oxide under alkali conditions. It was characterized by IR spectroscopy and X‐ray diffraction (XRD). We prepared alginate/HPCS blend fibers by spinning their solution through a viscose‐type spinneret into a coagulating bath containing aqueous CaCl2 and ethanol. The structure and properties of the blend fibers were studied with the aid of IR spectroscopy, scanning electron microscopy, and XRD. The results indicate a good miscibility between alginate and HPCS because of the strong interaction of the intermolecular hydrogen bonds. The mechanical properties and water‐retention properties were also measured. The best values of the tensile strength and breaking elongation of the blend fibers were obtained when the HPCS content was 30 wt %. The water‐retention values of the blend fibers increased as the amount of HPCS increased. Antibacterial fibers, obtained by the treatment of the fibers with an aqueous solution of silver nitrate, exhibited good antibacterial activity to Staphylococcus aureus. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
    Journal of Applied Polymer Science 07/2012; 125(2). · 1.40 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this article, we describe the first use of trisulfonated sodium amine as a sulfating reagent for preparing pectin sulfate in aqueous solution. The main reaction parameters that were expected to affect the degree of substitution (DS) were studied. The optimal reaction conditions for the synthesis of pectin sulfate were found to be as follows: the pH of the reaction medium, the reaction temperature, the reaction time, and the ratio of the mole of sodium nitrite (nNaNO2) to the mass of pectin (mpectin) were 6, 60°C, 12 h, and 2.5/190 mol/g, respectively. Pectin and pectin sulfate were characterized by Fourier transform infrared (FTIR) spectroscopy and 13C-NMR. The FTIR spectra showed the characteristic absorptions of sulfate ester bonds at 1264 and 830 cm−1. Furthermore, the anticoagulant activity of pectin sulfates with different DSs, concentrations, and molecular weights were investigated with respect to the activated partial thromboplastin time (APTT), thrombin time (TT) and prothombin time. The clotting assay indicated that the pectin sulfate prolonged APTT and TT through inhibition of the activity of antithrombin. These results suggest that the introduction of sulfate groups into the pectin structure improved its anticoagulant activity. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
    Journal of Applied Polymer Science 05/2012; 124(3). · 1.40 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cationic derivatives of pectin were prepared by reacting pectin with 3-chloro-2-hydroxypropyltrimethylammonium chloride (CHPTAC) in presence of sodium hydroxide (NaOH). The chemical structures of derivative were characterized by using elemental analysis, FT-IR, and 13C NMR spectroscopy. The results revealed that the degree of substitution (DS) of quaternized pectin (QP) could be controlled by adjusting the molar ratio of CHPTAC to pectin, the molar ratio of NaOH to CHPTAC and reaction temperature occurred during quaternization. The moisture absorption (Ra) and moisture retention (Rh) abilities of QP are closely related to the DS value. With the DS value increasing, Ra and Rh increased. In vitro antimicrobial activity assessment exhibited QP showed pronounced inhibitory effect against the three bacteria (Staphylococcus aureus, Escherichia coli, and Bacillus subtilis). The improved functionalities of the derivative might be explained by its polycationic characteristics.
    Carbohydrate Polymers 04/2012; 88(2):707–712. · 3.48 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Quaternary ammonium chitosan sulfates with diverse degrees of substitution (DS) ascribed to sulfate groups between 0.52 and 1.55 were synthesized by reacting quaternary ammonium chitosan with an uncommon sulfating agent (N(SO(3)Na)(3)) that was prepared from sodium bisulfite (NaHSO(3)) through reaction with sodium nitrite (NaNO(2)) in the aqueous system homogeneous. The structures of the derivatives were characterized by FTIR, (1)H NMR and (13)C NMR. The factors affecting DS of quaternary ammonium chitosan sulfates which included the molar ratio of NaNO(2) to quaternary ammonium chitosan, sulfated temperature, sulfated time and pH of sulfated reaction solution were investigated in detail. Its anticoagulation activity in vitro was determined by an activated partial thromboplastin time (APTT) assay, a thrombin time (TT) assay and a prothrombin time (PT) assay. Results of anticoagulation assays showed quaternary ammonium chitosan sulfates significantly prolonged APTT and TT, but not PT, and demonstrated that the introduction of sulfate groups into the quaternary ammonium chitosan structure improved its anticoagulant activity obviously. The study showed its anticoagulant properties strongly depended on its DS, concentration and molecular weight.
    International journal of biological macromolecules 01/2012; 50(1):31-7. · 2.37 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Oxidation of pectin was performed with sodium periodate to prepare pectin dialdehyde (PD). In this study we used the cross-linking reaction of the active aldehyde of PD and the amino of carboxymethyl chitosan (CMC) to prepare the hydrogels. By controlling the proportion of pectin dialdehyde and CMC we made different kinds of hydrogels. We systematically studied the characters of the hydrogels using Fourier transform infrared spectroscopy analysis of the pectin dialdehyde, CMC and the hydrogels, and also X-ray diffractometry and scanning electron microscopy analysis of the instrument of the hydrogels. Equilibrium swelling showed that the gels retained about 88-93% water. The water vapor transmission rate (WVTR) and the evaporation of water from gels showed that such hydrogels were optimal for maintaining a moist environment conducive for wound healing. Examination of the hemolytic potential showed that the hydrogels were nonhemolytic in nature. The hydrogels were non-toxic and blood-compatible. This hydrogel prepared from oxidized pectin and CMC without employing any extraneous cross-linking agents is expected to have potential as wound-dressing material.
    Journal of Biomaterials Science Polymer Edition 11/2011; · 1.70 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In order to develop a wound healing material possessing anticoagulant activity, antimicrobial activity and moisture absorbability and moisture-retention capacity, carboxymethyl κ-carrageenan (CMKC) was synthesized by the reaction of κ-carrageenan with monochloroacetic acid. The products were characterized by FT-IR, 13C NMR, degree of substitution (DS) and molecular weight. Anticoagulant activity of CMKC was investigated by APTT, TT and PT assays. The results showed that CMKC with a low DS promoted anticoagulant activity in comparison with κ-carrageenan, but as the DS further increased from 0.42 to 1.09, the activity decreased. Antibacterial activity was evaluated and we found that the introduction of carboxymethyl groups conferred antibacterial activity onto κ-carrageenan. Results indicated that CMKC exhibited good antimicrobial properties against Escherichia coli and Staphylococcus aureus, and the antibacterial activity of CMKC enhanced as the DS increased. CMKC displayed better moisture-absorption and water-retention ability than κ-carrageenan and as the DS increased, these properties of CMKC increased.
    Carbohydrate Polymers 08/2011; 86(3):1167–1174. · 3.48 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Sodium alginate sulfates prepared from sodium alginate through reaction with an uncommon sulfating agent (N (SO3Na)3) which was synthesized by sodium bisulfite and sodium nitrite in aqueous solution. The factors that could affect the degree of substitution (DS) of sodium alginate sulfates were investigated in detail. A sodium alginate sulfate with DS of 1.87 was obtained under optimal conditions. The structures of the derivatives were characterized by FTIR and 13C NMR. FTIR spectra showed the characteristic absorptions of sulfate ester bonds at 1249 cm−1 and 873 cm−1. The in vitro coagulation assay of human plasma containing the sodium alginate sulfates was determined with respect to activated partial thromboplastin time (APTT), thrombin time (TT) and prothombin time (PT). These activities strongly depended on the DS, molecular weight (Mw) and the concentration of sodium alginate sulfates. The introduction of sulfate groups to hydroxyl groups greatly prolonged the APTT and TT. Low S% and concentration sodium alginate sulfates showed little anticoagulant activity. The high DS and concentration could inhibit the activity of IIa and Xa to prolong APTT and TT. The low molecular weight resulted in higher anti-factor Xa activity to promote anticoagulant activity. Generally, the introducing of sulfate groups could not increase PT, it had little effect on coagulation factors in the extrinsic pathway.
    Carbohydrate Polymers. 01/2011;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A complex of chitosan (CS-40) and nisin (CS-40/nisin) was prepared and characterized with Fourier transform infrared spectroscopy and thermal analysis (thermogravimetry, differential thermogravimetry, and differential scanning calorimetry). The results show that the complex formed mainly by electrostatic interaction between the protonated amino group in CS-40 backbone with the carboxylate ion of nisin. Minimum inhibitory concentrations (MICs) were evaluated against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Bacillus stearothermophilus), Gram-negative bacteria (Escherichia coli, Salmonella enteritidis, and Proteus vulgaris), and fungi (Fusarium oxysporum). The results show that the CS-40/nisin solution did inhibit or even more strongly inhibited the growth of all the tested microorganisms, whereas CS-40 did not inhibit the growth of F. oxysporum and nisin did not inhibit the growth of Gram-negative bacteria (E. coli, S. enteritidis, and P. vulgaris). The relative inhibition times of CS-40/nisin solutions with different concentrations and ratios of CS-40 and nisin were also investigated against the seven microorganisms. The results showed that CS-40/nisin solutions with CS-40/nisin concentration ratios of 0.05/0.005, 0.05/0.0025, 0.05/0.00125, and 0.025/0.0001% had higher antimicrobial activity against all tested bacteria and fungi. The relationship between complex formation and antimicrobial activity is discussed. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
    Journal of Applied Polymer Science 02/2010; 116(6):3702 - 3707. · 1.40 Impact Factor

Publication Stats

294 Citations
66.16 Total Impact Points

Institutions

  • 2014
    • Hubei University of Science and Technology
      Hu-pei-ts’un, Shanxi Sheng, China
  • 2006–2014
    • Wuhan University of Technology
      • School of Materials Science and Engineering
      Wu-han-shih, Hubei, China
  • 2003–2011
    • Wuhan University
      • Department of Chemistry
      Wu-han-shih, Hubei, China