Publications (20)19.19 Total impact
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Dataset: Pesticide-Latif-2012
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Dataset: (A) Testicular and (B) epididymal sperm counts (mean ? SD) of rabbits treated with different doses of cypermethrin. CY mixed in mustard oil was injected IP to rabbits at weekly interval at 0 (control), 50, 100.and.150mg.kg-1bw.cypermethrin ip
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Article: Toxico-pathological effects of cypermethrin upon male reproductive system in rabbits
Pesticide Biochemistry and Physiology 05/2012; · 1.71 Impact Factor -
Article: Field evaluation of Eimeria tenella (local isolates) gametocytes vaccine and its comparative efficacy with imported live vaccine, LivaCox®
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ABSTRACT: The present paper describes the field evaluation of local gametocyte vaccine and its comparative efficacy with commercial anticoccidial vaccine, LivaCox®, used in breeder and broiler flocks in Pakistan. Humoral immune response in vaccinated and control chickens was monitored by enzyme-linked immunosorbent assay. Results demonstrated significantly elevated antibody titres in vaccinated groups as compared to control groups conducted both in Laboratory and field experiments. Significantly (P < 0.01) higher antibody titres in local gametocyte-vaccinated group as compared to LivaCox®-vaccinated chickens were recorded. Splenic cell migration inhibition assay was used to detect the cell-mediated immune (CMI) response, and results were expressed in terms of per cent migration index. Lower per cent migration index in LivaCox®-vaccinated chickens indicated the higher CMI response, as compared to local gametocyte-vaccinated chickens, although the difference was statistically non-significant (P > 0.05). Results of the challenge studies in laboratory experiments revealed significantly higher (P < 0.05) oocyst count in LivaCox®-vaccinated group as compared to local gametocyte-vaccinated chickens.Maximum protection (75%) against mixed species of genus Eimeria was recorded in chickens vaccinated with gametocyte vaccines as compared to LivaCox®-vaccinated group. The mean body weight gains in chickens vaccinated with local gametocyte vaccine were significantly better (P < 0.05) than in chickens vaccinated with LivaCox® vaccine, both in laboratory and field experiments. Majority of the chickens (70–72%) in control group demonstrated severe lesions (3.0–4.0), while 20–26% chickens showed moderate lesions (2.0). On the other hand, local gametocyte- and LivaCox®-immunized chickens developed 78% and 85% mild to moderated lesions (1.0–2.0), respectively. Results of the present study provide a probable explanation for cross-protection induced by Eimeria tenella gametocyte vaccines against other species of genus Eimeria.Parasitology Research 04/2012; 104(1):135-143. · 2.15 Impact Factor -
Article: Preparation and evaluation of chicken embryo-adapted fowl adenovirus serotype 4 vaccine in broiler chickens.
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ABSTRACT: The current study was planned to develop an efficient vaccine against hydropericardium syndrome virus (HSV). Currently, formalin-inactivated liver organ vaccines failed to protect the Pakistan broiler industry from this destructive disease of economic importance. A field isolate of the pathogenic hydropericardium syndrome virus was adapted to chicken embryos after four blind passages. The chicken embryo-adapted virus was further serially passaged (12 times) to get complete attenuation. Groups of broiler chickens free from maternal antibodies against HSV at the age of 14 days were immunized either with 16th passage attenuated HSV vaccine or commercially formalized liver organ vaccine. The antibody response, measured by enzyme-linked immunosorbent assay was significantly higher (P < 0.05) in the group immunized with the 16th passage attenuated HSV vaccine compared to the group immunized with liver organ vaccine at 7, 14, and 21 days post-immunization. At 24 days of age, the broiler chickens in each group were challenged with 10(3.83) embryo infectious dose(50) of pathogenic HSV and were observed for 7 days post-challenge. Vaccination with the 16th passage attenuated HSV gave 94.73% protection as validated on the basis of clinical signs (5.26%), gross lesions in the liver and heart (5.26%), histopathological lesions in the liver (1.5 ± 0.20), and mortality (5.26%). The birds inoculated with liver organ vaccine showed significantly low (p < 0.05; 55%) protection estimated on the basis of clinical signs (40%), gross lesions in the liver and heart (45%), histopathological lesions in the liver (2.7 ± 0.72), and mortality (35%). Birds in the unvaccinated control group showed high morbidity (84%), mortality (70%), gross (85%), and histopathological lesions (3.79 ± 0.14) with only 10% protection. In conclusion, this newly developed HSV vaccine proved to be immunogenic and has potential for controlling HSV infections in chickens.Tropical Animal Health and Production 09/2010; 43(2):331-8. · 1.12 Impact Factor -
Article: Molecular Characterization of Fowl Adenovirus Serotype 4 (FAV-4) Isolate Associated with Fowl Hydropericardium-Hepatitis Syndrome in Pakistan
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ABSTRACT: A study was conducted to characterize local fowl adenovirus serotype 4 (FAV-4) associated with hydropericardium syndrome (HPS) at molecular level. For this purpose, chicken livers naturally infected with HPS virus were aseptically collected from a field outbreak in commercial broilers from Faisalabad, Pakistan. Virus purification was done using a 15-45% (w/v) sucrose gradient. Virus was confirmed by agar gel precipitation test. Viral DNA was extracted and variable region hexon gene of 0.7 kb in size was amplified by PCR and sequenced. Nucleotide sequence (730 bp) and deduced amino acid sequence (243) of isolate was compared with already published sequences of FAV4. Sequence analysis of the variable region hexon gene confirmed that the virus belonged to avian adenovirus serotype 4. The nucleotide sequence of the Pakistani isolate had a 94% to 98% homology with Indian isolates, 97% homology with Belgium and Russian isolates and 96% homology with Canadian isolate. The amino acid sequences of the Pakistani isolate had an almost 87% to 98% homology with Indian, 92% and 95% homology with Belgian, 96% homology with Russian and 95% homology with Canadian isolate.Pakistan journal of zoology 01/2009; 41:269-276. · 0.34 Impact Factor -
Article: Adult B lymphoblastic leukaemia/lymphoma with hypodiploidy (-9) and a novel chromosomal translocation t(7;12)(q22;p13) presenting with severe eosinophilia – case report and review of literature
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ABSTRACT: Abstract Patients suffering from adult acute lymphoblastic leukemia are acutely ill and present most commonly with fever, pallor, bleeding, lymphadenopathy, hepatosplenomegaly and presence of lymphoblasts in the peripheral blood and bone marrow. We describe a rare presentation of acute lymphoblastic leukemia, in a young adult male who had vague and minimal symptoms with mild splenomegaly. There was severe eosinophilia along with absence of blasts in the peripheral blood, and 40% blasts with increase in eosinophils in the bone marrow. The blasts were positive for common precursor B cell markers on flow cytometry. The patient had a unique cytogenetic abnormality t(7;12)(q22;p13),-9, not previously described in acute lymphoblastic leukemia. He was categorized as poor risk due to failure to achieve complete remission after induction with UK ALL XII chemotherapy.Journal of Hematology & Oncology. 01/2009; -
Article: Appraisal of ethno-veterinary practices used for different ailments in dairy animals in peri-urban areas of Faisalabad (Pakistan)
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ABSTRACT: This study was conducted to document the ethno-veterinary practices (EVPs) used for treatment of different ailments in bovine and bubaline (dairy buffalo; Bubalus bubalis) livestock, over one year in peri-urban areas of Faisalabad. Rapid Rural Appraisal (RRA) and Participatory Rural Appraisal (PRA) techniques were used for selection of key-respondents (bovine & bubaline owners). Questionnaires with open-ended interviews were used to collect the information on EVPs used in bovine and bubaline for treatment of different ailments. There were a total of 58 EVPs, comprising of 41 based on plant usage, 11 combinations of plants and animal products/chemicals/organic matter and 6 based on animal products, chemicals or organic matter were documented for different ailments in dairy animals. Thirty-nine plant species representing 26 families were reported in the study area for treatment of different ailments in bovine and bubaline during past one year. Plants used in ≥2 conditions were Capsicum frutescens L., Withania coagulans (Stocks) Dund. (Solanaceaea); Eruca sativa Mill., Brassica campestris L. var. Sarson Prain, Lepidium sativum L., (Brassicaceae); Allium cepa L., Allium sativum (Liliaceae); Foeniculum vulgare Mill., Trachyspermum ammi L. Sprague ex Turill. (Apiaceae); Amomum subulatum Roxb. Zingiber officinale Rosc. (Zingiberaceae); Hordeum vulgare L. (Poaceae) and Piper nigrum L. (Piperaceae). The Remedies were prepared by pulverization, soaking in water and decoctions and administered per os or applied topically included pepper-corns, bulbs, flowers, leaves, rhizomes, seeds and fruits. Which are the commonly used part of plants. The EVPs that claimed to be effective need to be validated using scientific procedures and their quality, safety and standardization of doses should be assured.ISSN OnlineGNA. 01/2009; -
Article: Effects of cypermethrin on some clinico-hemato-biochemical and pathological parameters in male dwarf goats (Capra hircus).
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ABSTRACT: This study was carried out on 30 dwarf bucks to determine the effects of cypermethrin (CY) on clinical, hemato-biochemical and histopathological parameters. Animals were divided randomly into five equal groups, and each group was dipped in 0%, 0.1%, 0.4%, 0.8% or 1.6% CY, on days 0 and 15. Animals were monitored for clinical signs. Blood and serum samples were collected on day 0 and then fortnightly till day 75. Severe clinical signs comprising itching, restlessness, salivation, skin scratching and head shaking appeared at high doses (0.8% and 1.6% CY). Erythrocyte counts, hemoglobin, hematocrit, total protein, globulin and fibrinogen decreased significantly while total leukocyte counts, alanine aminotransferase and aspartate aminotransferase concentration increased significantly in all the treated groups. In the liver, necrosis of hepatocytes along with cytoplasmic vacuolation and fibroblasts proliferation were observed at a high dose of CY (1.6%). Microscopically kidneys showed congestion of parenchyma and condensation of epithelial cells of tubules along with deposition of casts in tubules. Shrinkage of glomerular capillaries and increased urinary spaces were pronounced in the high-dose group. Lungs exhibited accumulation of fibrinous exudation, thickening of alveolar walls, collapse and broken alveoli in animals treated with a high dose of CY. It was concluded that CY caused dose-dependent effects on all parameters studied. High doses of CY (0.8% and 1.6% solution) affected the parameters on erythrocytes and leukocytes for whole evaluation period, while effects on plasma proteins were transient and on ALT, AST and fibrinogen were transient but lasted a few weeks longer.Experimental and toxicologic pathology: official journal of the Gesellschaft fur Toxikologische Pathologie 10/2008; 61(2):151-60. · 1.43 Impact Factor -
Article: Prevalence of Campylobacter species in meat, milk and other food commodities in Pakistan.
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ABSTRACT: A surveillance study was carried out to determine the prevalence of Campylobacter in meat, milk and other food commodities in Pakistan. Over a period of 3 years (January 2002-December 2004), a total of 1636 food samples of meat, milk and other food commodities were procured from three big cities of Pakistan (Faisalabad, Lahore and Islamabad) and were analysed. Among meat samples, the highest prevalence (48%) of Campylobacter was recorded in raw chicken meat followed by raw beef (10.9%) and raw mutton (5.1%). Among other food commodities, the highest prevalence was observed in vegetable/fruit salad (40.9%), sandwiches (32%), cheese (11%) and raw bulk milk samples (10.2%). The overall prevalence of Campylobacter was found to be 21.5%, out of which 70.6% were identified as Campylobacter (C.) jejuni and 29.4% as C. coli. The study reported that the prevalence of Campylobacter spp. was significantly higher in the food commodities, which included raw/undercooked ingredients.Food Microbiology 06/2007; 24(3):219-22. · 3.28 Impact Factor -
Article: Immunogenic characterization of egg-adapted gametocytes of Eimera tenella.
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ABSTRACT: Egg-adapted gametocytes of Eimeria (E.) tenella (local isolates) were subjected to sodium dodecyl sulphate polyacrylimide gel electrophoresis under reducing conditions to fractionate the proteins. Immunogenicity of the gametocytes was detected by modified counter immunoelectrophoresis (MCIE) using convalescent sera from the outbreak cases of coccidiosis. Gametocytes of all the three isolates of E. tenella showed similar protein bands of molecular weights of 48.43, 27.20, 25.75, 22.57, and 12.92 kDa. MCIE recognized three precipitation bands with convalescent sera in comparison with the control. Results suggested that some of the 48.43-, 27.20-, 25.75-, 22.57-, and 12.92-kDa antigen(s) exist as soluble proteins in the parasite, which gave protection in our previous experiments against coccidiosis. Further studies on the biochemical characterization of the egg-adapted gametocyte antigen(s) and its role in E. tenella oocyst wall formation is underway.Parasitology Research 09/2006; 99(3):293-6. · 2.15 Impact Factor -
Article: Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks.
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ABSTRACT: Infectious bursal disease virus (IBDV) was inactivated by two different chemicals--formaldehyde and binary ethylenimine (BEI). Formaldehyde was used at 0.1% and 0.2%, while BEI was used at concentrations of 0.001 and 0.002 mol/L. These four vaccines were tested for their efficiency in generating humoral immune response in different groups of broiler chicks. Both BEI-inactivated vaccines gave relatively higher antibody titers and were almost twice as efficient as formaldehyde-inactivated ones.Journal of Zhejiang University SCIENCE B 09/2006; 7(8):660-4. · 1.10 Impact Factor -
Article: Protective effect of egg-propagated Eimeria tenella (local isolates) gametocytes as vaccine(s) against mixed species of coccidia in chickens.
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ABSTRACT: Egg propagated gametocytes of Eimeria tenella (local isolates) were used to prepare the adjuvanted (Amphigen) and nonadjuvanted vaccine(s) and evaluated on the basis of cellular, humoral, and challenge responses. Modified splenic cell migration inhibition test and enzyme-linked immunosorbant assay were used to assess the cellular and humoral responses, respectively. Chicken in groups A, B, C, and D were given adjuvanted vaccine (orally), adjuvanted vaccine (subcutaneously, s/c), nonadjuvanted vaccine (orally), and nonadjuvanted vaccine s/c, respectively. Control groups E, F, G, and H were given adjuvant (orally), adjuvant (s/c), phosphate-buffered saline (PBS) + adjuvant (orally), and PBS + adjuvant (s/c), respectively. On 5 and 15 days post vaccination after boosting, significantly higher (P<0.05) cell-mediated and humoral responses were detected in vaccinated chicken compared to control. No significant effect of adjuvant and vaccination route on the immune responses was found. Maximum percent protection (survivors after challenge) against mixed species of genus Eimeria was observed in group A (71.42%) followed by group C (63.63%), B (59.09%), and group D (54.54). Significantly higher (P<0.05) oocysts per gram (OPG) of droppings was observed in the control groups compared to the vaccinated chickens. Maximum percent reduction in OPG was also recorded in group A (86) followed by group C (84), group B (83), and group D (82). From these results, it was concluded that egg-propagated gametocytes (E. tenella) that gave protection upon challenge may be due to the control of E. tenella. Further studies on its feasibility as commercial vaccine are underway.Parasitology Research 06/2006; 98(6):539-44. · 2.15 Impact Factor -
Article: Preparation and evaluation of Vero-cell infectious bursal disease vaccine in Pakistan.
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ABSTRACT: The present work was carried out to develop an effective vaccine to control infectious bursal disease (IBD). The very virulent infectious bursal disease virus (vvIBDV) was adapted to grow in Vero-cell line after three serial passages. The virus was then attenuated by further serial passages and pathogenicity of different passages was determined in broiler chicks free from antibodies against IBDV. The ninth passage virus was completely attenuated, as it was non-pathogenic to chicks. The birds inoculated with ninth passage virus had bursa to body weight ratio comparable to un-inoculated control and zero mean lesion score. This ninth passage virus was evaluated as live attenuated Vero-cell adapted vaccine. A commercial live cell culture origin vaccine (IBD-VAC, Korea) was used for comparison. Experimental trials were conducted in 3-week-old broiler chicks. Clinical signs, gross and histopathological lesions and bursa to body weight ratio were all comparable to un-vaccinated control group in case of live attenuated Vero-cell vaccine (group A). High virus-neutralizing antibody titres were also found at 7, 14, and 21 days post-vaccination in group A. On challenge, 100% protection was achieved in group A and 65% in case of commercial vaccine (group B) in terms of bursal lesions. All the birds in control group showed sever bursal lesions post-challenge. The study indicated newly developed infectious bursal disease vaccine was found to be safe and immunogenic.Vaccine 05/2006; 24(15):2810-4. · 3.77 Impact Factor -
Article: The level of immunoglobulins in relation to neonatal lamb mortality in Pak-karakul sheep
Veterinarski Arhiv. 07/2000; 70:129-139. -
Article: Evaluation and comparison of Hydropericardium Syndrome vaccine in broiler chicks.
Pakistan Veterinary Journal. 06/1999; 19:88-90. -
Conference Proceeding: A modified Counter Immunoelctrophoresis Technique.
International seminar on Microbial Diseases of Livestock and Poultry., Lahore, Pakistan.; 03/1998 -
Article: Counter Immunoelectrophoresis - A rapid technique for diagnosis of Peste de Petits Ruminants
Pakistan Veterinary Journal. 03/1998; 18:55-56. -
Conference Proceeding: Impediments in biotechnology research in Pakistan
First Biotechnology Symposium - Agriculture Biotechnology, Faisalabad, Pakistan.; 06/1996 -
Article: Risk factors of lamb mortality in Pakistan
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ABSTRACT: This study was carried out to investigate lamb mortality in 528 Pak-Karakul and 423 Thalli in Pakistan during 1998–1999. Mortality was 9 and 12% in the lambs of the two breeds, respectively. In Pak-Karakul lambs, pneumonia rendered the highest morbidity and mortality and all mortalities recorded were in the first week, whereas in Thalli lambs diarrhoea was at the peak, with 82 and 18% mortalities in the first and second week of life respectively. Birth weight of neonates that died was lower ($P < 0$.05) than that of the lambs that had survived. The correlation revealed a positive and significant ($P = 0$.000) relationship between birth weights of Pak-Karakul lambs and serum Ig, total proteins and globulin concentration. In Thalli lambs such a relationship was found in serum Ig and globulin concentration. Parity of ewes and sex of lambs in both breeds did not affect mortality rates. Lambs with Ig levels less than 20 zinc sulphate turbidity (ZST) units died while those with more than 20 ZST-units survived. Dams that lost their lambs had significantly ($P < 0$.05) lower values of colostral Ig than those with live neonates. Serum Ig concentration in survived neonates of both breeds had significant ($P = 0$.000) and positive correlation with colostral Ig of their dams. Facteurs de risque de la mortalité des agneaux au Pakistan. Cette étude a été réalisée pour étudier la mortalité des agneaux de races Pak-Karakul (n = 528) et Thalli (n = 423) au Pakistan au cours des années 1998 et 1999. La mortalité était respectivement de 9 et 12 % pour les deux races. Pour les agneaux Pak-Karakul, la cause de morbidité la plus élevée a été la pneumonie et tous les cas de mortalité ont été enregistrés durant la première semaine de vie. Concernant les agneaux Thalli, la diarrhée a été à l'origine du plus grand nombre de décès, avec des mortalités de 82 et 18 % la première et la deuxième semaine de vie, respectivement. Le poids de naissance des nouveau-nés morts était inférieur ($P < 0$,05) à celui des agneaux qui ont survécu. La corrélation de Pearson a permis d'établir ($P = 0$,000) une relation positive et significative entre les poids à la naissance des agneaux Pak-Karakul et les immunoglobulines (Ig) du sérum, les protéines totales et la concentration en globulines. Chez les agneaux Thalli, une telle relation a été trouvée entre les Ig du sérum et la concentration en globulines. La parité des brebis et le sexe des agneaux dans les deux races n'ont pas affecté les taux de mortalité. Les agneaux avec des niveaux d'Ig de moins de 20 unités ZST (Zinc Sulphate Turbidity) sont décédés tandis que ceux avec plus de 20 unités ZST ont survécu. Les brebis ayant perdu leurs agneaux ont eu de manière significative ($P < 0$,05) un colostrum plus pauvre en Ig que celles avec des nouveau-nés vivants. Pour les deux races, il existe une corrélation significative et positive ($P = 0$,000) entre la concentration sérologique des Ig chez les nouveau-nés qui ont survécu et la teneur en Ig du colostrum de leur mère.http://dx.doi.org/10.1051/animres:2006017.
Top co-authors
Top Journals
Institutions
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2006–2012
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University of Agriculture Faisalabad
- Institute of Microbiology
Faisalābād, Punjab, Pakistan
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