Danuta Piatowska

Publications of Danuta Piatowska

• [Influence of topical fluoridation of glass ionomer cements on inhibitory activity on cariogenic bacteria].

  Authors: Małgorzata Płuciennik-Stronias, Danuta Sakowska, Zbigniew Krzemiński, Danuta Piatowska

  Medycyna doświadczalna i mikrobiologia. 01/2010; 62(4):369-74.

  Glass ionomer cements are important options in restorative and preventive dentistry due to their adhesion to the tooth surface and fluoride release, which can decrease the risk of recurrent caries.Glass ionomer cements are important options in restorative and preventive dentistry due to their adhesion to the tooth surface and fluoride release, which can decrease the risk of recurrent caries. The aim of this study was to define, in vivo, the influence of the topical use of fluoride gel on dental plaque bacteria growing on the glass ionomer cement. Fifteen patients were included into this study. Thirty five class V restorations from the glass ionomer cement (Ketac Molar Aplicap, ESPE Germany) were placed in the patient's one half of the lower jaw. The sound enamel of other side of the lower jaw was treated as a control. After 6 month 72 old dental plaque was collected from the surfaces of restorations and the surfaces of the sound enamel. Total amount of 30 dental plaque samples were investigated according to the previously described method (17). In dental plaque samples the amount of Streptococcus mutans was calculated at the Department of Microbiology, Medical University of Lódź. Next the topical application of fluoride gel (Fluormex) was performed on the surfaces of glass ionomer (Ketac Molar) fillings and the sound enamel. The patients were asked not to clean the teeth for 72 h. After this time the dental plaque was again collected from the surfaces of restorations and sound enamel. Statistical analysis of collected data was accomplished and showed no statistically significant differences in the amount of Streptococcus mutans both on Ketac Molar and the enamel before and after the topical use of fluoride gel. It was concluded that the topical fluoridation of glass ionomer cement did not affect Streptococcus mutans growing in dental plaque.

• [Assessment of the sealing ability of some root canal sealers applied using lateral condensation and single point techniques].

  Authors: Łukasz Suchodolski, Danuta Piatowska

  Annales Academiae Medicae Stetinensis. 01/2010; 56(1):81-6.

  To evaluate the sealing ability of AH Plus, Epiphany, and EZ-Fill Root Canal Cement and to assess leakage depending on the filling technique. Eighty extracted human single-root teeth were dividedTo evaluate the sealing ability of AH Plus, Epiphany, and EZ-Fill Root Canal Cement and to assess leakage depending on the filling technique. Eighty extracted human single-root teeth were divided into eight groups often teeth each. Thirty teeth were hand-filed to MAF 40 using a step-back technique and filled by lateral condensation with gutta-percha and AH Plus (group 1), 0.06 single point gutta-percha and AH Plus (group 2), and lateral condensation with Resilon/Epiphany (group 3). Another thirty teeth were prepared to size MAF 35 0.06 by rotary FlexMaster nickel-titanium instruments and filled using the single point technique with 0.06 Resilon/Epiphany (group 4), lateral condensation with gutta-percha and EZ-Fill cement (group 5), and 0.06 single point gutta-percha and EZ-Fill cement (group 6). The remaining twenty teeth served as positive and negative control groups. As positive control (group 7) served canals that were filed with the step-back technique and filled using lateral condensation of gutta-percha points without sealer. The negative control teeth (group 8) were left unfilled but completely covered with nail varnish. Microleakage was evaluated using a dye penetration test (2% solution of methylene blue for 72 h). The teeth were sectioned longitudinally and examined under a stereomicroscope to assess dye penetration. Differences between groups were analyzed statistically with Tamhane's test. The level of significance was taken as p < 0.05. Linear dye penetration (mean/SD) values (mm) were as follows: group 1 (1.38/2.14); group 2 (1.41/1.54); group 3 (8.98/4.55); group 4 (11.03/3.66); group 5 (1.95/2.22); group 6 (1.79/1.99). Leakage along the full length of the canal was noted in the positive control teeth and no leakage was found in negative control teeth. Tamhane's test showed that the sealing ability was lowest for Resilon/Epiphany. Leakage was highest for this material (groups 3 and 4) and was significantly higher than for teeth filled with gutta-percha combined with AH Plus or EZ-Fill. No significant differences were noted between gutta-percha with AH Plus (groups 1 and 2) and gutta-percha with EZ-Fill cement (groups 5 and 6). No significant difference in leakage existed between lateral condensation and single point groups. Leakage of the dye was greatest for the Resilon/Epiphany system. The results demonstrate that the sealing technique is without influence on how well the root canals will be sealed.

• [Influence of the fluoride releasing dental materials on the bacterial flora of dental plaque]

  Authors: Małgorzata Płuciennik, Danuta Sakowska, Zbigniew Krzemiński, Danuta Piatowska

  Medycyna doświadczalna i mikrobiologia. 02/2008; 60(2):131-8.

  The assessment of influence of silver-free, fluor releasing dental materials on dental plaque bacteria quantity. 17 patients were included into the study. 51 restorations were placed followingThe assessment of influence of silver-free, fluor releasing dental materials on dental plaque bacteria quantity. 17 patients were included into the study. 51 restorations were placed following manufacturers recommendations. Following materials were used: conventional glassionomer Ketac-Molar ESPE, resin modified glassionomer Fuji II LC GC and fluor containing composite Charisma Heraeus Kulzer Class V restorations were placed in following teeth of upper and lower jaw: canines, first bicuspids, second bicuspids. Sound enamel was a control. After 10 weeks the 72 hours old dental plaque was collected from surface of restorations and control using sterile probe. Total amount of 68 dental plaques were investigated. Each plaque was placed on scaled and sterile aluminum foil. The moist weight of dental plaque was scaled. Dental plaque was moved into 7 ml 0.85% NaCl solution reduced by cystein chlorine hydrogen and disintegrated by ultrasounds (power:100 Watt, wave amplitude: 5 micorm). The suspension of dental plaque was serially diluted from 10(-4) to 10(-5) in sterile 0,85% NaCl solution, and seeded with amount of 0.1 ml on appropriate base. In dental plaque trials the amount of cariogenic bacteria was calculated--Streptococcus mutans, Streptococcus, Lactobacillus, Veillonella and Neisseria, and also total amount of aerobic and anaerobic bacteria was measured. Microbiologic studies were performed in Institute of Microbiology, Medical University, Łódź. Statistical analysis of collected data was accomplished. In 72 hours old dental plaques collected from the surfaces of Ketac -Molar, Fuji II LC, Charisma after 10 weeks since being placed into the class V cavity, results show no statistically significant differences in the amount of Streptococcus mutans, Streptococcus spp., Lactobacillus spp., Veillonella spp., Neisseria spp, in total amount of aerobic and anaerobic bacteria and in the quantity proportion of Streptococcus mutans versus Streptococcus spp. in comparison with control trail. Results show no statistically significant differences in the amount of listed above bacteria and in the proportion of Streptococcus mutans versus Streptococcus spp. in 72 hours old dental plaques collected from surfaces of investigated restorative materials.

• An in vitro study of affected dentin as a risk factor for the development of secondary caries.

  Authors: Dariusz Borczyk, Danuta Piatowska, Zbigniew Krzemiński

  Caries research. 02/2006; 40(1):47-51.

  The objective of the study was to investigate the association between the presence of residual caries (inner affected dentin) on the cavity walls of cavity preparations and the further development ofThe objective of the study was to investigate the association between the presence of residual caries (inner affected dentin) on the cavity walls of cavity preparations and the further development of secondary caries lesions. Two 2 x 5 x 2 mm cavities, one in the apical portion (A) and one in the cervical portion (C) of the root, were prepared on both the lingual (L) and buccal (B) surfaces of 18 extracted human roots. The apical cavities (AB and AL) were artificially demineralized for 30 min using a decalcifying solution, followed by staining with a caries detector dye and then excavation of irreversibly demineralized dentin, leaving behind a layer of inner carious dentin. The cervical cavities (CB and CL) remained intact with sound dentin on the cavity walls. All cavities were then restored with composite resin. Following restoration, lingual specimens (AL and CL) were completely covered by an acid-resistant varnish to prevent further demineralization. All specimens were then incubated in an in vitro microbial artificial mouth model for 3 days in order to develop secondary carious lesions. At the end of the study all specimens were processed for energy-dispersive X-ray analysis of Ca concentration adjacent to the border between dentin and restoration. Statistical analysis of Ca concentrations revealed that the presence of affected inner dentin does not increase the susceptibility to secondary caries. Therefore, it was concluded that conservative cavity preparations leaving behind affected dentin do not increase the risk of secondary caries development.