Ali Unyayar

Publications (8)16.77 Total impact

• Article: Protective effect of Funalia trogii crude extract on deltamethrin-induced oxidative stress in rats

  Birgül Mazmanci, Mehmet Ali Mazmanci, Ali Unyayar, Serpil Unyayar, Fazilet Ozlem Cekic Aysin Guzel Deger, Serap Yalin, Ulku Comelekoglu
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  ABSTRACT: In this study the protective effects of cold buffer extract of Funalia trogii ATCC 200800 (FtE) and vitamin E (VitE) on oxidative stress induced with deltamethrin using oral administration in rats were investigated. Deltamethrin treatment caused an increase in liver enzyme activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) (p < 0.05); however, it caused a decrease in activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GRd) when compared to control group (p < 0.05). Activities of AST, ALT, ALP enzymes and level of thiobarbituric acid reactive substances (TBARS) decreased significantly after VitE administration (p < 0.05). Both enzyme activities and TBARS levels were found similar in VitE and FtE treated rats shortly after pesticide administration (p < 0.05). In conclusion, it appears that FtE prepared in cold buffer has capability to prevent the liver damage like VitE against the toxic effect of deltamethrin.
  Food Chemistry 01/2011; 125(3):1037–1040. · 3.65 Impact Factor
• Source

  Available from: academicjournals.org

  Article: Decolorization efficiency of Funalia trogii under static condition: Effect of C: N ratios

  Mehmet Ali Mazmanci, Ali Unyayar
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  ABSTRACT: Effects of physical conditions (pH and temperature), carbon and nitrogen source on decolorization of Reactive black 5 (RB5) by Funalia trogii were investigated under the static condition. Optimization of temperature and pH were also examined. Moreover, two different carbon sources (sucrose and starch), four different nitrogen sources and also four different C:N ratios (0.9, 3.0, 6.0, 18.0) were studied. Decolorization was expressed by mg dye / g dry mycelium weight. Optimal pH and temperature were found to be 4.78 and 30ºC, respectively. Decolorization efficiency increased with decreasing C:N ratio in starch-NH4H2PO4, starch-urea, sucrose-NH4H2PO4 and sucrose-urea containing media. Maximum decolorization was found as 9.61 and 7.77 mg dye/g dry mycelium weight in media containing no extra carbon and nitrogen sources. Kinetic studies were also carried out. The Lineweaver-Burk plot gave a Km of 406.66 mg/L and Vmax of 196.07 mg/L day for the diazo dye Reactive Black 5 decolorization by F. trogii under static condition.
  AFRICAN JOURNAL OF BIOTECHNOLOGY 01/2010; 9(39):6539-6544. · 0.57 Impact Factor
• Article: Thermophilic bacteria in cool temperate soils: are they metabolically active or continually added by global atmospheric transport?

  Roger Marchant, Andrea Franzetti, Spyros G Pavlostathis, Didem Okutman Tas, Isabel Erdbrugger, Ali Unyayar, Mehmet A Mazmanci, Ibrahim M Banat
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  ABSTRACT: Thermophilic soil geobacilli isolated from cool temperate geographical zone environments have been shown to be metabolically inactive under aerobic conditions at ambient temperatures (-5 to 25 degrees C). It is now confirmed that a similar situation exists for their anaerobic denitrification activity. It is necessary therefore to determine the mechanisms that sustain the observed significant viable populations in these soils. Population analysis of thermophiles in rainwater and air samples has shown different species compositions which support the view that long distance global transport and deposition in rainwater is a possible source of replenishment of the soil thermophile populations. Survival experiments using a representative Geobacillus isolate have indicated that while cells lose viability rapidly at most temperatures, populations can increase only when the temperature allows growth to take place at a rate which exceeds death rate. Long term (9-month) experiments at 4 degrees C show population increases which can be accounted for by very slow growth rates complemented by negligible death rates. These results are interpreted in the context of current hypotheses on the biogeography patterns of bacteria.
  Applied Microbiology and Biotechnology 05/2008; 78(5):841-52. · 3.42 Impact Factor
• Article: Evaluation of cytotoxic and mutagenic effects of Coriolus versicolor and Funalia trogii extracts on mammalian cells.

  Ali Unyayar, Murat Demirbilek, Melisa Turkoglu, Ayla Celik, Mehmet A Mazmanci, Emrah A Erkurt, Serpil Unyayar, Ozlem Cekic, Hatice Atacag
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  ABSTRACT: This study examined the in vitro cytotoxic activities of standardized aqueous bioactive extracts prepared from Coriolus versicolor and Funalia trogiiATCC 200800 on HeLa and fibroblast cell lines using a MTT (3-[4,5-dimetiltiazol-2-]-2-5-difeniltetrazolium bromide) cytotoxicity assay. F. trogii and C. versicolor extracts were cytotoxic to both cell lines. At 10 microL treatment level, F. trogii and C. versicolor extracts inhibited proliferation of HeLa cancer cells by 71.5% and 45%, respectively, compared with controls. Toxicity was lower toward normal fibroblasts. In the latter case, treatment at 10 microL level with F. trogii and C. versicolor extracts reduced cell proliferation by 51.3% and 38.7%, respectively. In separate experiments, the mitotic index (MI) obtained with 3 microL treatment level of unheated extracts of the two fungi was comparable to the MI value obtained by treatment with 4 microg/mL MMC (anticancer agent mitomycin-C). A significant induction of sister chromatid exchange (SCE) was observed in normal cultured lymphocytes treated with MMC (4 microg/mL). MMC treatment reduced replication index compared with treatment with unheated F. trogii extract and negative controls (p < 0.001). In contrast to MMC, F. trogii extracts did not affect the proliferation of human lymphocytes compared with controls (p > 0.05). Laccase and peroxidase enzyme activities in F. trogii extract were implicated in their inhibitory effect on cancer cells. F. trogii extract was concluded to have antitumor activity.
  Drug and Chemical Toxicology 01/2006; 29(1):69-83. · 1.08 Impact Factor
• Article: Decolourisation of reactive textile dyes Drimarene Blue X3LR and Remazol Brilliant Blue R by Funalia trogii ATCC 200800.

  H Duygu Ozsoy, Ali Unyayar, M Ali Mazmanci
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  ABSTRACT: Decolourisation of reactive dyes Drimarene Blue X3LR and Remazol Brilliant Blue R by white rot fungi Funalia trogii was studied under static conditions. The effect of various conditions such as mycelial age, initial dye and glucose concentrations on decolourisation were also investigated. Decolourisation activity of F. trogii was compared with Phanerochaete chrysosporium known as test microorganism. It was found that 7-day-old cultures were more effective than 5-day-old cultures of F. trogii for decolourisation of these dyes. Decolourisations by F. trogii of both dyes were increased with glucose concentration decreasing. In contrast, decolourisations by P. chrysosporium were decreased. F. trogii decolourised 92-98% of both dyes within 4-10 h. However, P. chrysosporium partiallydecolourised (11-20%) these dyes during 10 days incubation period under the same conditions.
  Biodegradation 07/2005; 16(3):195-204. · 2.02 Impact Factor
• Article: Decolourisation of Reactive Black 5 by Funalia trogii immobilised on Luffa cylindrica sponge

  Mehmet Ali Mazmanci, Ali Unyayar
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  ABSTRACT: The decolourisation of Reactive Black 5 (RB5) by immobilised Funalia trogii was investigated. Cultures of F. trogii immobilised on Luffa cylindrica sponge could effectively decolourise the dye. The effect of mycelial age was also studied, and decolourisation rate of a 3-day-old age culture was higher (8.22 mg dye/g dmw day) than those of 0- and 6-day-old cultures (6.86 and 7.80 mg dye/g dmw day). Macroscopic and microscopic examinations showed that dye was not biosorbed on the fungal mycelium. The growth of F. trogii was inhibited by all tested dye concentrations with compared to controls but this effect was minimised when the fungus was completely immobilised on the sponge. Using optimal mycelial age, cultures of L. cylindrica sponge were tested for their ability for dye decolourisation at different initial concentration. The kinetic parameters of decolourisation were calculated according to Lineweaver–Burk plots (Km of 106.04 mg dye/l and Vmax of 117.64 mg dye/l day).
  PROCESS BIOCHEMISTRY 01/2005; 40(1):337-342. · 2.63 Impact Factor
• Article: Production of Remazol Brilliant Blue R decolourising oxygenase from the culture filtrate of Funalia trogii ATCC 200800

  Tayfun Deveci, Ali Unyayar, Mehmet A Mazmanci
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  ABSTRACT: Decolourisation of Remazol Brilliant Blue R, an azo textile dyestuff, by crude filtrate of Funalia trogii ATCC 200800 growing in solid-state fermentation (SSF) medium containing wheat bran and soybean hull was studied. Optimum pH and temperature for laccase and horseradish like peroxidase (HRP) production in SSF medium were determined at 5 and 30 °C, respectively. Maximum enzyme synthesis was found in 10 days old cultures. We also found Remazol Brilliant Blue R decolourising enzymatic activity in the culture filtrate of F. trogii. The optimum pH and temperature for enzymatic decolourisation were determined at 3.0 and 50 °C, respectively. Both veratryl alcohol and peroxide ions (H2O2) accelerated the peroxidase enzyme reactions, whereas decelerated the decolourisation of RBBR with the culture filtrate of F. trogii. Sodium azide (NaN3), cysteine and sodium cyanide (NaCN) inhibited RBBR decolourising activity, laccase and peroxidase activities. When sodium metabisulphite (Na2S2O5) was used as an inhibitor, a significant inhibition of laccase and dye decolourising enzyme activities but no peroxidase activity was observed. Initial colourless and later orange bands were obtained by the activity staining process with RBBR and laccase substrate (guaicol), respectively, after separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of this band was estimated as about 65 kDa by SDS-PAGE. Since the reaction was catalysed in the absence of H2O2 as co-substrate, it was concluded that this enzyme was a laccase.
  Journal of Molecular Catalysis B Enzymatic 01/2004; 30(1):25-32. · 2.73 Impact Factor
• Article: Decolorization of Methylene Blue by White Rot Fungus Coriolus versicolor

  Mehmet Ali Mazmanci, Ali Unyayar, Hacı İbrahim Ekiz
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  ABSTRACT: Decolorization of Methylene Blue in cultures of the white rot fungus, Coriolus versicolor, was demonstrated. C. versicolor was found to effectively decolorize media containing 5 and 10 mg L-1 methylene blue. The effects of various glucose (5.5 and 27.7 mM)and NH4H2PO4 (4.34, 0.43 and 0.04 mM) concentrations on the decolorization of methylene blue by C. versicolor were investigated. Decolorizing was found higher in media where the C/N ratio was adjusted to 2:1. Maximum decolorizing activity was achieved during the secondary metabolic phase.
  Fresenius Environmental Bulletin 01/2002; 11(5). · 0.66 Impact Factor