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Katsuyuki Mitomo,
Uta Griesenbach,
Makoto Inoue,
Lucinda Somerton,
Cuixiang Meng,
Eiji Akiba,
Toshiaki Tabata,
Yasuji Ueda,
Gad M Frankel,
Raymond Farley,
Charanjit Singh,
Mario Chan,
Felix Munkonge,
Andrea Brum,
Stefania Xenariou, Sara Escudero-Garcia,
Mamoru Hasegawa,
Eric W F W Alton
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ABSTRACT: Gene therapy for cystic fibrosis (CF) is making encouraging progress into clinical trials. However, further improvements in transduction efficiency are desired. To develop a novel gene transfer vector that is improved and truly effective for CF gene therapy, a simian immunodeficiency virus (SIV) was pseudotyped with envelope proteins from Sendai virus (SeV), which is known to efficiently transduce unconditioned airway epithelial cells from the apical side. This novel vector was evaluated in mice in vivo and in vitro directed toward CF gene therapy. Here, we show that (i) we can produce relevant titers of an SIV vector pseudotyped with SeV envelope proteins for in vivo use, (ii) this vector can transduce the respiratory epithelium of the murine nose in vivo at levels that may be relevant for clinical benefit in CF, (iii) this can be achieved in a single formulation, and without the need for preconditioning, (iv) expression can last for 15 months, (v) readministration is feasible, (vi) the vector can transduce human air-liquid interface (ALI) cultures, and (vii) functional CF transmembrane conductance regulator (CFTR) chloride channels can be generated in vitro. Our data suggest that this lentiviral vector may provide a step change in airway transduction efficiency relevant to a clinical programme of gene therapy for CF.
Molecular Therapy 03/2010; 18(6):1173-82. · 6.87 Impact Factor
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ABSTRACT: It is not known whether the progressive airway changes in cystic fibrosis (CF) are all secondary to infection and inflammation. The CF mouse nose shares electrophysiologic and cellular properties with human CF airway epithelium. In the present work, we tested the hypothesis that structural abnormalities in the nasal mucosa of CF mice develop independent of infection and inflammation. We performed nasal lavage and subsequent serial coronal section through the nasal tissue of adult CF (mutations Cftr(TgHm1G551D) and Cftr(tm1Unc)-TgN((FABPCFTR))) and wild-type mice raised under normal housing conditions. Nasal tissue was also obtained from Day 17 embryos and newborn pups. Detailed histologic examination of the respiratory and olfactory epithelium within the nasal cavity was performed. Bacterial culture, cell count, and macrophage inflammatory protein-2 (MIP-2) concentration were assessed in nasal lavage fluid. Significantly thickened respiratory epithelium and increased mucous cell density was found in adult CF mice of both mutations compared with wild-type animals. In contrast, the olfactory epithelium was thinner, with a decreased cell density. Areas of lymphoid aggregates were found in CF mice but not in non-CF mice. There were no differences in bacterial growth, cell count, or MIP-2 concentrations. No genotype differences were observed in the embryonic or newborn periods. There are significant histologic changes in the nasal mucosa of adult CF mice, not associated with increased lumenal inflammation or bacterial content, and which are not present perinatally. These may be novel therapeutic targets.
American Journal of Respiratory Cell and Molecular Biology 08/2008; 39(1):19-25. · 5.13 Impact Factor
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Uta Griesenbach,
Chris Kitson, Sara Escudero Garcia,
Raymond Farley,
Charanjit Singh,
Luci Somerton,
Hazel Painter,
Rbecca L Smith,
Deborah R Gill,
Stephen C Hyde,
Yu-Hua Chow,
Jim Hu,
Mike Gray,
Mark Edbrooke,
Varrie Ogilvie,
Gordon MacGregor,
Ronald K Scheule,
Seng H Cheng,
Natasha J Caplen,
Eric W F W Alton
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ABSTRACT: The cationic lipid Genzyme lipid (GL) 67 is the current "gold-standard" for in vivo lung gene transfer. Here, we assessed, if GL67 mediated uptake of siRNAs and asODNs into airway epithelium in vivo.
Anti-lacZ and ENaC (epithelial sodium channel) siRNA and asODN were complexed to GL67 and administered to the mouse airway epithelium in vivo Transfection efficiency and efficacy were assessed using real-time RT-PCR as well as through protein expression and functional studies. In parallel in vitro experiments were carried out to select the most efficient oligonucleotides.
In vitro, GL67 efficiently complexed asODNs and siRNAs, and both were stable in exhaled breath condensate. Importantly, during in vitro selection of functional siRNA and asODN we noted that asODNs accumulated rapidly in the nuclei of transfected cells, whereas siRNAs remained in the cytoplasm, a pattern consistent with their presumed site of action. Following in vivo lung transfection siRNAs were only visible in alveolar macrophages, whereas asODN also transfected alveolar epithelial cells, but no significant uptake into conducting airway epithelial cells was seen. SiRNAs and asODNs targeted to beta-galactosidase reduced betagal mRNA levels in the airway epithelium of K18-lacZ mice by 30% and 60%, respectively. However, this was insufficient to reduce protein expression. In an attempt to increase transfection efficiency of the airway epithelium, we increased contact time of siRNA and asODN using the in vivo mouse nose model. Although highly variable and inefficient, transfection of airway epithelium with asODN, but not siRNA, was now seen. As asODNs more effectively transfected nasal airway epithelial cells, we assessed the effect of asODN against ENaC, a potential therapeutic target in cystic fibrosis; no decrease in ENaC mRNA levels or function was detected.
This study suggests that although siRNAs and asODNs can be developed to inhibit gene expression in culture systems and certain organs in vivo, barriers to nucleic acid transfer in airway epithelial cells seen with large DNA molecules may also affect the efficiency of in vivo uptake of small nucleic acid molecules.
Respiratory research 02/2006; 7:26. · 3.36 Impact Factor
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Uta Griesenbach, Sara Escudero Garcia,
Chris Kitson,
Raymond Farley,
Charanjit Singh,
Hazel Painter,
Rebecca L. Smith,
Ron Scheule,
Seng Cheng,
Mark Edbrooke,
Steve Hyde,
Duncan M. Geddes,
Eric W. F. W. Alton
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ABSTRACT: Molecular Therapy (2004) 9, S192–S193; doi: 10.1016/j.ymthe.2004.06.445
507. Down-Regulation of the Epithelial Sodium Channel ENaC through Antisense Oligonucleotides
Uta Griesenbach1,5, Sara Escudero Garcia1,5, Chris Kitson2, Raymond Farley1,5, Charanjit Singh1,5, Hazel Painter3,5, Rebecca L. Smith3,5, Ron Scheule4, Seng Cheng4, Mark Edbrooke2, Steve Hyde3,5, Duncan M. Geddes1,5 and Eric W. F. W. Alton1,51Department of Gene Therapy, Faculty of Medicine, Imperial College, London, United Kingdom2GlaxoSmithKline, Stevage, United Kingdom3Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, Oxford, United Kingdom4Genzyme Corp, Framingham5UK Cystic Fibrosis Gene Therapy Consortium, United Kingdom
Molecular Therapy 04/2004; · 6.87 Impact Factor
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Uta Griesenbach,
Rosemary Boyton,
Luci Somerton, Sara Escudero Garcia,
Stefano Ferrari,
Toshiyuki Owaki,
Shu Tsugumine,
Duncan M Geddes,
Mamoru Hasegawa,
Danny Altmann,
Eric W F W Alton
