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Publications (8)3.13 Total impact

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    ABSTRACT: Molecular studies of European classical rabies viruses (RABV) have revealed a number of geographically clustered lineages. To study the diversity of Balkan RABV, partial nucleoprotein (N) gene sequences were analysed from a unique panel of isolates (n = 210), collected from various hosts between 1972 and 2006. All of the Balkan isolates grouped within the European/Middle East Lineage, with the majority most closely related to East European strains. A number of RABV from Bosnia & Herzegovina and Montenegro, collected between 1986 and 2006, grouped with the West European strains, believed to be responsible for the rabies epizootic that spread throughout Europe in the latter half of the 20th Century. In contrast, no Serbian RABV belonged to this sublineage. However, a distinct group of Serbian fox RABV provided further evidence for the southwards wildlife-mediated movement of rabies from Hungary, Romania and Serbia into Bulgaria. To determine the optimal region for evolutionary analysis, partial, full and concatenated N-gene and glycoprotein (G) gene sequences were compared. Whilst both the divergence times and evolutionary rates were similar irrespective of genomic region, the 95 % highest probability density (HPD) limits were significantly reduced for full N-gene and concatenated NG-gene sequences compared with partial gene sequences. Bayesian coalescent analysis estimated the date of the most common recent ancestor of the Balkan RABV to be 1885 (95 % HPD, 1852-1913), and skyline plots suggested an expansion of the local viral population in 1980-1990, which coincides with the observed emergence of fox rabies in the region.
    Journal of General Virology 06/2011; 92(Pt 9):2171-80. · 3.13 Impact Factor
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    ABSTRACT: The Lyssavirus genus, a member of the Rhabdoviridae family, consists of seven established related viruses (genotypes 1-7). Rabies cases in Eurasia are principally attributed to three of these genotypes, namely genotype 1 (RABV, classical rabies) and to a lesser extent genotypes 5 and 6 (European bat lyssaviruses type-1 and -2). In addition, four newly identified divergent lyssaviruses have been isolated from insectivorous bats. The molecular diversity of classical rabies viruses (genotype 1, RABV) has been studied at the global level and reference has been made to the existence of a number of European strains in a range of mammalian species. It is accepted that these viruses cluster within a 'Cosmopolitan Lineage' having ancestral roots in Europe in the 17th century before its widespread dispersal to Asia, Africa and the Americas as a result of European exploration and colonization.
    Developments in biologicals 01/2008; 131:125-31.
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    ABSTRACT: Several different strains of classical rabies virus co-circulate in Europe. In order to investigate the roles of the host species and topography on the molecular epidemiology of these viruses, a 400 bp region of the nucleoprotein gene was sequenced and compared with more than 500 European virus isolates. Viruses from 21 European countries were represented including some unique panels of archived isolates from the former Republic of Yugoslavia, Estonia, the Czech Republic, Poland and Austria. Phylogenetic analysis of 198 unique sequences demonstrated numerous groups of viruses clustered at both geographical and host-species levels.
    Developments in biologicals 02/2006; 125:17-28.
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    ABSTRACT: Application of the rabies immunoglobuline is a compulsory part of the prophylaxis of rabies in all severe, transdermal lesions caused by rabies infected animals. Sylvatic rabies has spread in the past few years throughout the whole Yugoslavia, and human cases of rabies have also been reported in other East European countries. In order to achieve the highest level of rabies prophylaxis, apart from postinfective rabies vaccination, it is necessary to provide passive immunization using specific antibodies against rabies. After successful immunization of the young, healthy volunteers in 1990, National Blood Transfusion Institute, in cooperation with the Pasteur Institute from Novi Sad, prepared the first quantities of immunized plasma by plasmapheresis procedure and human rabies immunoglobuline. Without national production, sufficient quantities of human rabies immunoglobuline could not be provided, since the price on the world market is rather high (over $1000 per patient).
    Medicinski pregled 02/2001; 54 Suppl 1:33-7.
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    ABSTRACT: In this study we analyzed 36 cases of dog rabies diagnosed at the Pasteur Institute in Novi Sad during the last 10 years as well as the quality of postvaccination immunity. Most dogs had the paralytic form, more common in sylvatic rabies than furious clinical picture. 11% of dogs were aggressive, whereas 44% had injured humans, including their owners. Postvaccination immunity was examined in a group of dogs vaccinated with three domestic and one vaccine imported from abroad. Vaccination was successful in more than 70% of dogs and it is a sufficient percentage of seroconversion in dog rabies eradication. However, in dogs vaccinated only once during lifetime, the immunity was not satisfactory. That is why in primary vaccination of dogs compulsory revaccination 30 days after the first vaccine dose is suggested.
    Medicinski pregled 02/1998; 51 Suppl 1:43-6.
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    ABSTRACT: The production of rabies vaccine on baby hamster kidney (BHK-21) cells for human use is discussed. Long term experience in application of this vaccine in animals, without any noticeable complications and findings of inactivation of contaminated DNA from the cell substrate by beta-propiolactone have justified its recommendation for human use. Preliminary results of applying this simple, adjuvant vaccine in volunteers, confirmed its good tolerability and immunogenicity.
    Medicinski pregled 02/1998; 51 Suppl 1:17-9.
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    ABSTRACT: The Federal Republic of Yugoslavia is situated on the border of a great sylvatic rabies epizootics occurring in central and eastern Europe. That is the reason why a relatively small number of rabid animals have been registered. In 1997, 124 rabid animals, mainly red foxes, were identified. In recent years red fox rabies epizootics in Yugoslavia has annually spread 15-20 km to the south. Rabies in domestic animals, especially dogs, was characterized with furious clinical forms and dog-to-dog transmission typical of urban rabies. Sylvatic rabies has been almost completely eradicated in some countries of western Europe in the last years, mostly due to the method of oral vaccination of foxes-the main vector of rabies.
    Medicinski pregled 02/1998; 51 Suppl 1:31-3.
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    ABSTRACT: By producing the rabies vaccine from cell culture, this vaccination has become safe, with minimal postvaccinal reactions. The first vaccine according to this technology was produced by Pavle (Paul) Fenje, former chief of department of the Pasteur Institute in Novi Sad. Many cell cultures have been introduced so far for the rabies virus multiplication: primary hamster kidney, fetal bovine kidney, chick embryo, continuous cell line monkey kidney (VERO), human diploid cell (HDC), etc. Some possibilities of an economical rabies vaccine production from a continuous BHK-21 cell line have been discussed and recommended.
    Medicinski pregled 01/1997; 50(11-12):565-8.