Jing Lan

Publications (2)5.68 Total impact

• Article: Quantitation of bergenin in human plasma by liquid chromatography/tandem mass spectrometry.

  Weisong Yu, Yingwu Wang, Yunhui Zhang, Dan Zhang, Jing Lan, Zeyuan Liu, Jingkai Gu, J Paul Fawcett
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  ABSTRACT: This paper reports the development and validation of an assay for quantitation of bergenin in human plasma using liquid chromatography/tandem mass spectrometry (LC-MS/MS). Bergenin and the internal standard (I.S.), 5-bromo-2,4(1H,3H)-pyrimidinedione (5-BrU), were separated by reversed phase HPLC and quantitated by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) in the negative ion mode. The most intense [M-H](-) MRM transition of bergenin at m/z 326.9-->312.3 was used for quantitation and the transition at m/z 188.9-->42.2 was used to monitor 5-BrU. Stability issues with bergenin required the addition of ascorbic acid to plasma samples prior to storage and analysis within 10 days storage at -80 degrees C. The method was linear in the range 3-1000 ng/mL with intra- and inter-day precision of 3.94-5.96 and 1.62-8.31%, respectively, and accuracy <2.33%. The assay was successfully applied to a pharmacokinetic study in healthy volunteers after administration of a single 250 mg oral dose.
  Journal of Chromatography B 12/2008; 877(1-2):33-6. · 2.89 Impact Factor
• Article: Liquid chromatographic/tandem mass spectrometric method for the quantitation of tranilast in human plasma.

  Jing Lan, Jian Wang, Dan Wu, Yingwu Wang, J Paul Fawcett, Jingkai Gu
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  ABSTRACT: An analytical method for the determination of tranilast in human plasma using tramadol as the internal standard has been developed based on liquid chromatography/tandem mass spectrometry. Sample preparation involved protein precipitation with methanol. Separation by reversed-phase high-performance liquid chromatography using methanol/10 mM ammonium acetate (70: 30, v/v) as mobile phase was complete in a run time of 2.4 min. Detection on a Q TRAP system used multiple reaction monitoring. The method was linear in the range 0.06-20 microg/mL with intra- and inter-day precisions (as relative standard deviation) of 2.2-2.6% and 2.3-2.9%, respectively. Accuracy (as relative error) was <-2.5%. The method was applied in a pharmacokinetic study in healthy volunteers treated with a single 80 mg oral dose of tranilast.
  Rapid Communications in Mass Spectrometry 02/2006; 20(22):3309-12. · 2.79 Impact Factor