S A Levitskiĭ

Publications (2)0 Total impact

• Article: [Molecular genetic methods of typing of the Bartonellae].

  M Iu Kirillov, A P Markov, I V Lopyrev, V N Pankratova, S A Levitskiĭ, V N Bashkirov, G B Smirnov, A N Kruglov, V A Osadchaia, G P Frolova, G V Barmina, O A Morozova, M Y Kosoy
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  ABSTRACT: The primer systems for the PCR detection of four house-keeping genes of bartonellae in clinical material were developed and tested. The tactics of the species RFLP typing was also developed and tested. The scheme of the species RFLP typing of bartonellae was tested using as an example two strains for the first time isolated in Russia from patients with endocarditis and fever of uncertain origin. The results of the typing were supported by sequencing of the amplicons obtained. According to the sequencing the isolates were attributed to the sub species Bartonella vinsonii, subsp. arupensis. The necessity of molecular epidemiological analysis of bartonelloses in Russia was substantiated.
  Molekuliarnaia genetika, mikrobiologiia i virusologiia 02/2007;
• Article: [Wild small mammals are the reservoir hosts of the Bartonella genus bacteria in the south of Moscow region].

  A P Markov, I V Lopyrev, A I Irkhin, L A Khliap, S A Levitskiĭ, M Iu Kirillov, V A Manuvera, T S Il'ina, M S Pokrovskaia, G I Aleshkin, V N Bashkirov, G B Smirnov, M Kosoĭ
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  ABSTRACT: A total of 103 blood samples collected from wild small mammals captured in the Prioksko-Terrasny Reserve on the south of Moscow region were studied to determine the bartonellae prevalence. The examined species were the yellow-necked mice Apodemus flavicollis (35 samples), the European wood mouse Apodemus uralensis (10 samples), the bank vole Clethrionomys glareolus (51 samples), the house mouse Mus musculus (3 samples), the common vole Microtus arvalis (2 samples), and the shrew Sorex araneus (2 samples). Initially, we obtained 76 bacterial Bartonella-like isolates after plating onto the surface of the solid nutrient media. 66 of them were PCR-positive at least for three of four targets, gltA, ftsZ, ribC and 16S RNA. Thus, the percentage of the infection in the studied community was 64%. Subsequent RFLP assay showed that obtained isolates belonged to the Bartonella grahamii and/or B. taylorii species. In 7 cases we found both bartonellae species in one animal. These data were confirmed by direct sequencing of four ftsZ, four ribC and two gltA amplicons. According to our data, there is no any marked host specificity for these bartonellae species. Now we have laid the bartonellae strain collection consisting of 31 isolates. To our knowledge, this is the first investigation of the bartonellae prevalence in wild small mammals performed in Russia. The comparison of our data with those obtained by European researchers and issues of coinfection by different bartonellae species and host specificity are discussed.
  Molekuliarnaia genetika, mikrobiologiia i virusologiia 02/2006;