T Ben Chaabene

La Rabta Hospital Tunis, Tunis-Ville, Tūnis, Tunisia

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Publications (9)6.58 Total impact

  • R Abdelmalek · S Anane · N Chabchoub · R Essid · K Aoun · T Ben Chaabéne · A Bouratbine
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    ABSTRACT: Microsporidiosis and cryptosporidiosis are emerging opportunistic infections responsible for intestinal manifestations that are often severe in immunocompromised patients. A case of microsporidiosis-cryptosporidiosis coinfection is reported in an HIV-infected newborn. The patient was a 17-day-old female, exclusively breastfed and with no contact with animals. Microsporidiosis and cryptosporidiosis were diagnosed after systematic screening in stool samples using both specific staining and PCR. Two species of microsporidia, Encephalitozoon intestinalis and Enterocytozoon bieneusi, and Cryptosporidium hominis were identified. The contamination of the newborn probably resulted from direct human-to-human transmission during close contact with the mother (who had diarrhea and refused stool sampling). This report highlights the usefulness of the screening of intestinal microsporidiosis and cryptosporidiosis in HIV-infected subjects for better management.
    Archives de Pédiatrie 03/2011; 18(5). DOI:10.1016/j.arcped.2011.02.016 · 0.41 Impact Factor
  • N Chabchoub · R Abdelmalek · S Issa · F Kanoun · T Ben Chaabene · A Bouratbine · K Aoun
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    ABSTRACT: Intestinal microsporidiosis are among the most frequent opportunistic diseases in immunocompromised subjects. This study aimed to evaluate the contribution of PCR for a better detection and species identification of microsporidia in stool specimens of HIV-infected patients. Stool samples obtained from 119 HIV-infected Tunisian subjects were screened for intestinal microsporidiosis by light microscopy using Weber's modified Trichrome stain and by a PCR method using universal primers V1/PMP2 which amplified a common fragment of the small subunit rRNA gene of microsporidia. The obtained PCR products were then sequenced using an ABI PRISM 377 DNA sequencer. The results showed a better sensitivity of PCR in the detection of microsporidia with an infection rate of 14.3% significantly higher than that of 6.7% obtained by light microscopy (p=0.03). As previously described, intestinal microsporidiosis was associated with low CD4 cell counts; 23.9% infection rate in patients having CD4 cell count under 200/mm(3) against 5.6% in patients with higher CD4 cell count (p=0.008). The sequencing of 15 out of the 17 positive PCR products has confirmed in all cases the species identified based on the PCR fragment size i.e., 250pb for Enterocytozoon bieneusi (seven cases) and about 270pb for Encephalitozoon intestinalis (nine cases); one case revealed a double infection. PCR proved to be more effective than classical Trichrome stain for the diagnosis of intestinal microsporidiosis. Moreover, the ability of PCR to identify the species involved could also be useful for cases management.
    Pathologie Biologie 11/2009; 60(2):91-4. DOI:10.1016/j.patbio.2009.07.034 · 1.07 Impact Factor
  • Médecine et Maladies Infectieuses 06/2009; 39. DOI:10.1016/S0399-077X(09)74383-5 · 0.91 Impact Factor
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    ABSTRACT: Complement is part of the innate immune system and underlies one of the main effector mechanisms of antibodymediated immunity. It has three physiological activities: defending against pyogenic bacterial infection, bridging innate and adaptive immunity and disposing immune complexes with production of inflammatory injury [1]. Many types of complement system deficiency can cause an increase of the susceptibility to infections. A deficiency that compromises the opsonic activities causes a general susceptibility to pyogenic organisms; any deficiency that compromises the lytic activity can increase the susceptibility to neisserial infections. However, the susceptibility to intracellular pathogens has never been reported in patients with complement deficiency [2]. C1 inhibitor (C1-INH) is the main regulatory protein
    Infection 05/2008; 36(2):181-3. DOI:10.1007/s15010-007-7217-2 · 2.86 Impact Factor
  • A Toumi · B Kilani · L Ammari · H Tiouiri · F Kanoun · S Belhadj · E Chaker · T Ben Chaabene
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    ABSTRACT: The purpose of this study was to evaluate in a retrospective analysis, cases of Mediterranean visceral leishmaniasis (VL) diagnosed in adults during a 20-year period in a department of infectious diseases. Demographic data, clinical and laboratory features and therapeutic findings were considered. During the study period, 22 cases of VL were diagnosed, and 6 (27%) were associated with HIV infection. Fever and splenomegaly were observed in all cases. Anaemia was constant. The anti-leishmanial IF titer was positive among 21 patients (95%). Smears from bone marrow aspiration were positive at microscopy in 95% of cases. Zymodeme analysis was carried out in nine isolates. L. infantum zymodeme MON-1 was characterized in all cases. Seventeen patients (77%) received meglumine antimoniate (MA) (20 mg SbV/kg per day) and 5 (23%) patients amphotericin B (AB) (0.5-1 mg/kg per day) for an average period of 25 days (10-49 days). Adverse events occurred in 7 patients (32%), among them 4 received AB. Clinical cure was achieved with success in 21 patients (95%). After a successful MA treatment of the initial episode, VL relapse was observed in one HIV-positive patient. Only one HIV-positive patient died from neurological disorders. VL is rare in adults. However, its incidence is increasing everywhere in the world, because of HIV-related cases. Its prognosis depends on the precocity of diagnosis and treatment.
    Bulletin de la Société de pathologie exotique 11/2007; 100(4):282-6.
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    ABSTRACT: To determine the prevalence of antibodies to hepatitis C virus (HCV) in a cohort of patients infected with human immunodeficiency virus 1 (HIV-1) and to compare risk factors. Multicenter retrospective study from infectious diseases department in Tunisia. We examined sera from HIV patients followed in these centers. Diagnosis of HCV infection was based on third generation enzyme-linked immunosorbent assay (ELISA) test. 362 HIV-1 patients were included in the study. The mean age was 35,5 years. 272 patients (75.13%) were male. Sexual transmission of HIV was the main risk factor (56.3%). 144 patients (39.7%) had antibodies against HCV, mainly in patients with history of intravenous drug abuse (78.4%). Quantitative evaluation of hepatitis C virus RNA was done only in 3 patients. HCV-HIV coinfection in tunisian patients occurs frequently, due to the same ways of transmission. More studies are needed to focus on sexual transmission of hepatitis C virus in order to prevent such infections rather than interferon-based therapies.
    La Tunisie médicale 03/2007; 85(2):121-3.
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    ABSTRACT: Sixty one Tunisian adult patients with bacterial meningitis were screened for complement deficiency. Functional activity of the classical and the alternative pathways of complement (CH50 and AP50 respectively) were measured according to standard haemolytic procedures. Serum concentrations of C3 and C4 were determined by nephelometry. Late complement component (C5-C9) and properdin concentrations were assessed by double-ligand EISA. Complement deficiency was found in eight patients (13%): Seven had late complement component deficiency (three C7 deficiency, two C5 deficiency, one C6 deficiency and one C8 deficiency) and one had partial properdin deficiency. Patients with late complement component deficiency had a mean age of 24 years (range 17-32 years). All deficient patients had meningococcal meningitis. Recurrent meningitis was reported in half of the patients. Our findings demonstrated a high prevalence of complement deficiency in Tunisia suggesting that screening for hereditary complement deficiency should be performed in case of bacterial meningitides and meningococcal disease patients.
    Archives de l'Institut Pasteur de Tunis 02/2006; 83(1-4):25-34.
  • Badreddine Kilani · Lamia Amari · Slah Belhaj · Ahmed Goubontini · Taoufik Ben Chaabene
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    ABSTRACT: The reference treatment in visceral leishmaniasis is administration of antimonial compounds. Failures have been reported particularly in HIV-positive patients. The authors describe the case of a 40-year-old patient who, after 2 courses of N-methyl-glucamine, relapsed. Bone marrow cultures became negative only after adminstration of amphotericin B (1 mg x kg x d) for 28 days. He is still asymptomatic 18 months after he was cured. Amphotericin B, in case of relapse, seems to be a valuable alternative, until the availability of the liposomal form in our country.
    La Tunisie médicale 04/2004; 82(3):316-9.
  • B Kilani · L Ammari · H Tiouiri · A Goubontini · F Kanoun · F Zouiten · T.-B Chaabène
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    ABSTRACT: Purpose. – Central nervous system (CNS) tuberculosis remains a public health problem, particularly in developping countries.The aim of this study is to characterize neuroradiologic findings of various intracranial lesions.Methods. – We retrospectively reviewed data of 122 patients with CNS tuberculosis, without immunosuppression. CT scan was performed in all patients, whereas 17 patients had CT scan and MRI.Results. – We included 74 women (61%) and 48 men (39%) with a mean age of 37 years (17 -88y). 18 patients (14,7%) had a history of tuberculosis. Tuberculous meningitis was the most frequent clinical presentation (119 cases). Mycobacterium tuberculosis was isolated in cerebrospinal fluid of 18 patients (15%). Several types of lesions were identified : hydrocephalus (35 cases), tuberculomas (29 cases), leptomeningitis (26 cases), infarction (15 cases), abcesses (2 cases). Hydrocephalus was associated to other lesions in 26 cases. Communication hydrocephalus was present in 28 cases. Multiple tuberculomas were seen in 23 cases (80%), with miliary aspects in some cases. In 3 cases, tuberculoma was present without meningitis. Patients with leptomeningitis showed thick meningeal contrast enhancement involving all basal cisterns. Infarction resulted from arterial englobement or embols, and involved the area of middle cerebral artery (12 cases).Conclusion. – Central nervous system tuberculosis has different appearences, mostly hydrocephalus and tuberculomas. MR with contrast is necessary for diagnosis and for follow-up during treatment.
    La Revue de Médecine Interne 02/2003; 24(2):86-96. DOI:10.1016/S0248-8663(02)00716-6 · 1.32 Impact Factor