Zhisheng Jiang

Nankai University, Tianjin, Tianjin Shi, China

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Publications (2)3.67 Total impact

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    ABSTRACT: An indirect competitive immunoassay for the insecticide parathion has been optimized and characterized. This assay is based on a monoclonal antibody (2H9) produced from an immunogen, a bovine thyroglobulin (BTG) conjugate wherein the reduced form of parathion was multiply bound to the carrier protein via diazo bonds. Assay was performed in the parathion-HSA coated (0.25 μg/ml) ELISA format in which antibody was diluted 1:2000. Several physicochemical factors (pH, ionic strength, BSA concentrations and organic solvent) that influence assay performance were studied and optimized. Finally, the assay was applied to the analysis of parathion in spiked vegetable samples. The sensitivity, estimated as the IC50 value, was 360 ng/ml, with a practical working range between 47 and 6000 ng/ml, a limit of detection of 26 ng/ml, and inter-assay and intra-assay variations less than 10%. The average recovery of parathion added to potato, celery and Chinese cabbage were 173 ± 34%, 108 ± 15% and 98 ± 6%, respectively.
    Food Chemistry 01/2007; · 3.33 Impact Factor
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    ABSTRACT: An amino-derivative of parathion was prepared and conjugated to human serum albumin (HSA) and bovine thyroglobulin (BTG) via diazonium condensation. Spleen cells producing high titer antibody were removed and fused with myeloma cells of SP2/0 origin. Using a conventional immunization protocol, we generated nine stable murine monoclonal antibodies (MAbs) producing cell lines to parathion. After four successive limiting dilutions, antibodies produced by nine clones had high affinities, ranging from 10(9) to 10(12) M(-1). These clones were found to be of IgG class and IgM class with k light chain. Subclass determination showed that the clones produced IgG(1), IgG(2a), IgG(2b), and IgM types of antibody. One clone (2H(9)) was used to establish the calibration curve with a sensitivity of 26 ng/mL, a practical working range of 46.8-6000 ng/mL parathion.
    Hybridoma (2005) 01/2006; 24(6):298-304. · 0.33 Impact Factor

Publication Stats

22 Citations
3.67 Total Impact Points

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Institutions

  • 2007
    • Nankai University
      • Department of Chemistry
      Tianjin, Tianjin Shi, China
  • 2006
    • Molecular and Cellular Biology Program
      • Laboratory of Cellular and Molecular Biology
      Seattle, Washington, United States