[Show abstract][Hide abstract] ABSTRACT: AimThe aim of this single-center, two-cell, double-blind, randomized controlled clinical study was to evaluate the effectiveness of an in-office desensitizing paste containing 8% arginine and calcium carbonate in providing relief on dentine hypersensitivity immediately after scaling and root planing and its sustained relief over a 6-week period.Materials and Methods
Fifty periodontitis subjects presenting hypersensitivity were subjected to scaling and root planing and in-office application of either 8% arginine and calcium carbonate desensitizing paste (25 subjects, test group) or fluoride-free prophylaxis paste (25 subjects, control group). Air-blast hypersensitivity was assessed using Schiff and Visual Analog (VAS) scales at baseline, post-scaling, post-prophy, 2-, 4-, 6-weeks.ResultsAt all evaluation times, the test group presented significant % reduction in hypersensitivity relative to post-scaling (t-test, p < 0.05) (Schiff:Test: 57, 58.6, 60.2, 68, Control: 28.6, 22.2, 23, 23) (VAS: Test: 60, 55.6, 60.1, 68.4, Control: 25.9, 18.2, 20.6, 22.7) and significant % hypersensitivity difference relative to control (ANCOVA, p < 0.05) (Schiff: 38.9, 45.9, 47.4, 57.7, VAS: 49.1, 48.9, 52.6, 61).Conclusions
The single in-office application of the 8% arginine-calcium carbonate desensitizing paste after scaling and root planing provided significant immediate reduction of dentine hypersensitivity, which sustained over a 6-week period.This article is protected by copyright. All rights reserved.
[Show abstract][Hide abstract] ABSTRACT: Objective: This single-center, two-cell, double-blind randomized clinical study evaluated the efficacy of an arginine-based desensitizing paste in providing relief on dentin hypersensitivity up to a 6-week post-treatment period in periodontitis patients.
Method: Fifty subjects with periodontitis and dentin hypersensitivity in at least 2 teeth were randomly assigned to two fluoride-free paste treatment groups: (a) Colgate Sensitive Pro-Relief® in-office (test), (b) Nupro-M pumice (control). For each subject clinical periodontal parameters were recorded before (baseline) and 6 weeks (final) after scaling and root planing. Two 3-sec applications of the pastes were performed after scaling and root planing. Subjects were examined for dentin hypersensitivity (DH) in air-blast stimulus using Schiff (S) and Visual analog (V) scales before scaling (baseline), after scaling, immediately after paste treatment and at 2, 4 and 6 weeks. Paired t-tests, covariance (ANCOVA’s) and Pearson correlation tests were used for the statistical analysis (p<0.05).
Result: The two groups did not significantly differ in baseline DH, baseline and final periodontal measurements and in improvement of periodontal parameters with treatment. Test paste significantly reduced DH relative to baseline immediately after placement (S:48,1; V:40%), at 2 (S:50.0; V:33.5%), 4 (S:51.9; V:40.1%) and 6 (S:61.3; V:52.7%) weeks. For the control paste, there was no significant effect in DH immediately after placement (S:13.5; V:-3.0%), at 2 (S:5.8; V:-13.8%), 4 (S:6.7; V:-10.4%) and 6 (S:6.7; V:-7.5%) weeks. Strong correlation (≥0.70) was demonstrated between clinical attachment level and probing depth as well as between S and V scales both at baseline and 6 weeks.
Conclusion: The single professional application of the Colgate Sensitive Pro-Relief® paste significantly reduced dentin hypersensitivity over a period of 6 weeks.
[Show abstract][Hide abstract] ABSTRACT: In the present study we investigated the incidence of bacteriocins produced by 236 lactic acid bacteria (LAB) food isolates against pathogenic or opportunistic pathogenic oral bacteria. This set of LAB contained several strains (≥17%) producing bacteriocins active against food-related bacteria. Interestingly only Streptococcus macedonicus ACA-DC 198 was able to inhibit the growth of Streptococcus oralis, Streptococcus sanguinis and Streptococcus gordonii, while Lactobacillus fermentum ACA-DC 179 and Lactobacillus plantarun ACA-DC 269 produced bacteriocins solely against Streptococcus oralis. Thus, the percentage of strains that were found to produce bacteriocins against oral bacteria was ~1.3%. The rarity of bacteriocins active against oral LAB pathogens produced by food-related LAB was unexpected given their close phylogenetic relationship. Nevertheless, when tested in inhibition assays, the potency of the bacteriocin(s) of S. macedonicus ACA-DC 198 against the three oral streptococci was high. Fourier-transform infrared spectroscopy combined with principal component analysis revealed that exposure of the target cells to the antimicrobial compounds caused major alterations of key cellular constituents. Our findings indicate that bacteriocins produced by food-related LAB against oral LAB may be rare, but deserve further investigation since, when discovered, they can be effective antimicrobials.
International Journal of Molecular Sciences 03/2013; 14(3):4640-54. DOI:10.3390/ijms14034640 · 2.86 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The aim of this interventional animal study was to assess histologically the effect of experimental diabetes in rats with experimental periodontitis in terms of alveolar bone loss and the effect of experimental periodontitis on glucose levels in diabetes.
Forty-seven Wistar rats were studied: 12 healthy controls (C), 10 with experimental diabetes (D), 12 with experimental diabetes and experimental periodontitis (DP) and 13 with experimental periodontitis (P). Diabetes was induced by streptozotocin injection and periodontitis was induced at the right second maxillary molar by ligation. Serum glucose levels were measured at specific time points. Sixty-one days after ligation, the rats were sacrificed. Histometric analysis assessed alveolar crest level. For ligated groups, alveolar bone loss was expressed as the difference in alveolar crest level between right and left maxillary molars.
Diabetes alone did not statistically significantly affect alveolar crest level. The combination of diabetes and periodontitis caused greater alveolar bone loss (946.1 +/- 719.9 microm) than periodontitis alone (639.7 +/- 294.2 microm); however, the difference did not reach statistical significance. Periodontitis did not significantly increase glucose levels in diabetic rats. The average glucose levels were 545.4 (499 - 563) and 504.5 (445 - 560) mg/dL for diabetic and diabetic ligated rats, respectively.
Within its limits, this study demonstrated that the severity of alveolar bone loss in periodontitis was not significantly aggravated by diabetes and the serum glucose levels in diabetes were not affected by periodontitis.
Journal of the International Academy of Periodontology 04/2012; 14(2):35-41.
[Show abstract][Hide abstract] ABSTRACT: The aim of the study was to compare the serum levels of adiponectin and interleukin-6 (IL-6) in insulin-treated diabetic rats with or without periodontitis. Forty male Wistar rats were randomly divided into 2 groups (20 rats each): a) insulin-treated diabetic group (control, DI) and b) insulin-treated diabetic periodontitis group (test, DIP). Diabetes was induced, and insulin treatment was initiated on day 5. On day 16, periodontitis was induced in the DIP group. All rats were euthanized on day 77. Adiponectin and IL-6 were assessed on days 16 and 77. At the end of the experiment, 14 and 11 rats survived in the DI and DIP groups, respectively. Adiponectin levels were statistically significantly higher at the end of the experiment compared with levels on day 16 in the periodontitis group (p < 0.05), but not in the control group. At the end of the experiment, adiponectin levels were statistically significantly higher in the periodontitis group compared with the control group (p < 0.05). Within-group and between-group comparisons of IL-6 levels showed no statistically significant difference. In conclusion, serum adiponectin was increased in insulin-treated diabetic rats with periodontitis in comparison with insulin-treated diabetic rats, while IL-6 levels did not differ between groups.
Brazilian oral research 02/2012; 26(1):71-6. DOI:10.1590/S1806-83242012000100012 · 0.94 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: This study assessed root proximity (RP) in patients with and without periodontitis in terms of prevalence, distribution, location along the root, width and severity, and explored the role of RP characteristics in the type of alveolar bone loss (horizontal or angular).
Root proximity was studied in the interdental spaces of 250 patients with periodontitis and 80 patients without disease. Linear measurements were performed in digitized radiographs. Root proximity was classified by location along the root, width and severity. Bone defect type (horizontal or angular) at the RP site was recorded.
Root proximity prevalence did not differ between periodontitis and non-periodontitis groups. For both groups, most RPs were located at the middle root third. Root proximity width and severity in periodontitis sites were different between horizontal and angular bone loss sites. Root proximity width was greater in horizontal bone loss sites. In periodontitis, a unit (pixel) increase in the RP width decreased the probability to detect an angular bone defect by 20%, while a unit decrease in RP severity increased the possibility to detect angular bone loss by 71%.
Root proximity prevalence was similar for both patients with and without periodontitis. The RP location along the root was not related to the existence of periodontitis. Root proximity width and severity differed between horizontal and angular bone loss sites. Root proximities had greater width in horizontal than angular bone loss sites.
Journal of the International Academy of Periodontology 10/2011; 13(3):73-9.
[Show abstract][Hide abstract] ABSTRACT: To evaluate the efficacy of the DenShield desensitizing system, based on calcium sodium phosphosilicate, in the hypersensitivity reduction for a 6-month period in periodontitis patients previously subjected to periodontal treatment and to compare the combination of the in-office paste and at-home dentifrice use to the at-home dentifrice use alone.
A total of 248 teeth (eight teeth in each subject) in 31 periodontitis patients (mean age 48 +/- 8 years) previously subjected to periodontal treatment were studied. 193 (77.8%) teeth had been treated with phase I periodontal treatment alone (non-surgical treatment) and 55 (22.2%) had been additionally subjected to periodontal surgery. Periodontal clinical parameters were recorded for each subject. Hypersensitivity was assessed by tactile and air-blast stimuli. The hypersensitive teeth of each of two quadrants in each subject were randomly assigned with split-mouth design to in-office application of DenShield Starter paste (four teeth) or placebo (distilled water) (four teeth). After the in-office application each patient used the DenShield dentifrices (Builder and Saver) for 6 months. The final evaluation was at 6 months.
The prevalence and the degree of baseline hypersensitivity was significantly higher for the surgically than the non-surgically-treated teeth (83.6% versus 68.4%) and it was greater in teeth with attachment loss. The dentin hypersensitivity observed after periodontal treatment was significantly reduced in periodontitis patients who used the DenShield system for 6 months. There was no difference in hypersensitivity reduction between the additional in-office application of the DenShield and the at-home use of the DenShield dentifrices alone.
American journal of dentistry 04/2011; 24(2):85-92. · 0.85 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Oral and perioral piercing is the penetration of the oral
mucosa and the perioral skin and the placement of
ornament into the opening. Oral piercing is an increasingly
popular habit among young people. Tongue, lips and
cheeks are the most common sites for piercing.
The purpose of the present review was to explore the literature
and analyze the literature data concerning the oral
piercing and especially the oral piercing complications in
the oral cavity.
Oral piercing entails risk for the oral health and the general
health. It might present early complications in the oral cavity,
namely pain, edema, hemorrhage and inflammatory
reaction at the piercing site.
In the long term it might lead to late complications in the oral
cavity, namely abnormal toothwear and toothchipping/-
cracking, gingival recession, localized periodontal tissue
loss, increased salivary flow, difficulties in chewing and
speaking, soft tissue overgrowth and change in the
microbial flora at the piercing site.
Furthermore, complications concerning the general health
have been reported, such as Ludwigs angina, endocarditis,
airway obstruction and systemic infection.
The factors influencing the development and severity of
oral piercing complications have not been thoroughly
[Show abstract][Hide abstract] ABSTRACT: On the basis of systematic reviews and randomized controlled trials, the authors provide reports of two cases in which platelet-rich plasma (PRP) combined with demineralized freeze-dried bone allograft (DFDBA) was used to treat periodontal endosseous defects.
Clinicians treated two circumferential endosseous defects with a probing pocket depth of 5 and 8 millimeters, respectively (case 1), and a combined 1-2-3-wall endosseous defect with a probing pocket depth of 6 mm (case 2) by using the combination of PRP and DFDBA. At six months, complete periodontal pocket resolution occurred in all defects, and clinical attachment level and radiographic defect fill in all defects exhibited significant improvement compared with presurgical values.
The combination of PRP and DFDBA may be clinically and radiographically efficacious in the treatment of periodontal endosseous defects.
Journal of the American Dental Association (1939) 08/2010; 141(8):967-78. DOI:10.14219/jada.archive.2010.0310 · 2.01 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Platelet-rich plasma (PRP) alone or combined with other regenerative materials was previously studied in human periodontal endosseous defects. There are no sufficient data evaluating to what extent the addition of demineralized freeze-dried bone allograft (DFDBA) to PRP may enhance the effectiveness of PRP. The aim of this randomized, double-masked, controlled clinical trial was to compare the effectiveness of autologous PRP alone to PRP + DFDBA in periodontal endosseous defects.
Twenty-four proximal endosseous defects in 24 patients with severe chronic periodontitis were randomly treated with PRP alone or in combination with DFDBA. The final evaluation at 6 months was based on clinical and radiographic parameters. Subtraction radiography was used. The primary outcome variable was clinical attachment level (CAL).
The two treatment groups were initially comparable (mean CAL: 8.67 +/- 2.19 mm for PRP + DFDBA and 8.25 +/- 1.96 mm for PRP). Both treatments achieved statistically significant and similar CAL gain (3.08 +/- 1.17 mm for PRP + DFDBA and 3.08 +/- 0.95 mm for PRP), probing depth, defect depth, and area surface reduction. The percentage of defect fill did not significantly differ between the two treatments. There was a non-significant trend to greater defect fill (45.42% versus 41.29%), defect depth (54.05% versus 49.52%), and area surface (58.43% versus 52.16%) reduction with the graft. In both groups, 66.66% of the defects gained > or =3 mm of CAL.
Within its limits, this study demonstrates that both PRP and PRP combined with DFDBA resulted in significant clinical and radiographic improvement in human periodontal endosseous defects at 6 months, and the addition of DFDBA to PRP did not significantly enhance the treatment outcome.
Journal of Periodontology 12/2009; 80(12):1911-9. DOI:10.1902/jop.2009.090216 · 2.71 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Investigators have evaluated predictive parameters of tooth loss during the maintenance phase (MP). The authors conducted a retrospective study to evaluate the rate of tooth loss and to explore the parameters that affect tooth loss during MP in a Greek population.
A periodontist administered periodontal treatment and maintenance care to 280 participants with severe periodontitis for a mean period +/- standard deviation of 10.84 +/- 2.13 years. The periodontist recorded the following parameters for each participant: oral hygiene index level, simplified gingival index level, clinical attachment level, probing depth measurements, initial tooth prognosis, smoking status, tooth loss during active periodontal treatment and MP, and compliance with suggested maintenance visits.
The authors found that total tooth loss during active treatment (n = 1,427) was greater than during MP (n = 918) and was associated with the initial tooth prognosis, tooth type group, participants' compliance with suggested maintenance visits, smoking status and acceptability of the quality of tooth restorations. Most of the teeth extracted during maintenance had an initial guarded prognosis (n = 612). Participants whose compliance was erratic had a greater risk of undergoing tooth extraction than did participants whose compliance was complete.
Participants' initial tooth prognosis, tooth type, compliance with suggested maintenance visits and smoking status affected tooth loss during MP. Initial guarded prognosis and erratic compliance increased the risk of undergoing tooth extraction during maintenance.
Determining predictive parameters for disease progression and tooth loss provides critical information to clinicians so that they can develop and implement rational treatment planning.
Journal of the American Dental Association (1939) 10/2009; 140(9):1100-7. DOI:10.14219/jada.archive.2009.0336 · 2.01 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: The purpose of this study was to evaluate the gingival fibroblast proliferative response derived from patients with chronic and aggressive periodontitis to homologous platelet-rich plasma (PRP).
Gingival fibroblasts derived from nine patients with chronic periodontitis, aggressive periodontitis and healthy periodontium were grown. Medium was replaced with DMEM containing 0.5% FBS in which cells remained for two days. Cells were incubated and cultured with medium containing 50 microl/ml homologous platelet-rich plasma (PRP) or not containing PRP (control) for 24 and 48 hours. PRP originated from three donors. Cell proliferation effect was evaluated at 24 and 48 hours. Cell viability was assessed with a hemocytometer. Viable cells were counted under a phase contrast microscope.
The results revealed that incubation of human gingival fibroblasts, derived from healthy and intact periodontium, chronic periodontitis and aggressive periodontitis, in culture medium containing homologous PRP statistically significantly increased the cell proliferation at 24 and 48 hours of culture.
The addition of PRP to human gingival fibroblast cultures significantly increased the proliferative response, irrespective of the presence of periodontitis, type of periodontitis and PRP donor.
Journal of the International Academy of Periodontology 02/2009; 11(1):160-8.
[Show abstract][Hide abstract] ABSTRACT: In periodontal regeneration, the growth factor concentrations and the delivery system used are of great importance. In an attempt to assess the mitogenic effect of basic fibroblast growth factor (bFGF) on periodontal ligament (PDL) cells combined with different bone replacement materials, two allografts of cortical (DFDBA) and cancellous (DFBA) bone and an anorganic bovine material with a synthetic peptide (ABM P-15) were used. The purpose of this study was to evaluate the in vitro mitogenic effect of different doses of bFGF alone or in combination with DFDBA, DFBA and ABM P-15 on human PDL cells in a time-dependent mode.
PDL cell cultures were derived from the mid-root of four maxillary premolars. Cells were grown and reached confluence. On day 2 of quiescence, new medium was added along with (1) 1, 5, 10 and 25 ng/ml of bFGF alone, (2) 10 mg of DFDBA, DFBA and ABM P-15 alone and (3) their combination. The mitogenic effect was determined at 24 and 48 h of culture by using a hemocytometer chamber. The cells were counted under a phase contrast microscope.
The results revealed that bFGF at the highest concentrations and after 48 h exerted a significant mitogenic effect on PDL cells, and also DFDBA and DFBA supported cell proliferation. Furthermore, DFDBA and DFBA enriched with bFGF had a significant mitogenic effect after 48 h of culture. ABM P-15 with 10 and 25 ng/ml of bFGF up-regulated PDL cell proliferation after 48 h of incubation.
The findings of this study demonstrate the beneficial role of bFGF combined with DFDBA and DFBA as carriers in periodontal repair.
[Show abstract][Hide abstract] ABSTRACT: The purpose of this study is to investigate the correlation between the degree of patient compliance with the suggested periodontal maintenance treatment intervals and the new root caries development 5 years after the completion of the active periodontal treatment. The percentage of root surfaces with new root caries was low for both the erratic and the complete compliance patient groups. Erratic compliance with the suggested maintenance intervals 5 years after the active periodontal treatment leads to greater root caries prevalence, greater percentages of patients and root surfaces with new root caries, greater root caries prevalence for smokers, and greater percentages of new defects in root surfaces with further clinical attachment loss and plaque retention. The prevention of root caries development is based on the daily plaque removal and the high patient compliance with the therapist's suggestions for periodontal maintenance intervals.
Compendium of continuing education in dentistry (Jamesburg, N.J.: 1995) 01/2006; 26(12):835-44; quiz 845.