In the present study, Cu(II)-imprinted polymer was prepared and used as an effective method for separation and enrichment of Cu (II) ion in samples. An on-line preconcentration and extraction flow-injection system using solid phase extraction (SPE) column packed with prepared ion imprinted polymer (IIP) was set up. The column was eluted with a mixture of ethanol and H2 SO4 after preconcentration. A sensitive and simple chemiluminescence (CL) method using flow-injection has been developed for the determination of copper. The method is based on the fact that traces of Cu(II) exert a catalytic action on the oxidation between morin by KMnO4 solution. After optimizing the different experimental parameters, a calibration graph was obtained over the concentration range of 0 to 10 microg x mL(-1) for Cu(II) and r = 0.996 8. The detection limit is 0.001 2 microg x mL(-1). The recoveries are between 95% and 105% and the standard relative deviations (RSD) are less than 3% at 4.0 microg x mL(-1) (n = 9) level was obtained. The new chemiluminescence determination for copper has been established. The method can be satisfactorily applied to the determination of copper in sample.
Guang pu xue yu guang pu fen xi = Guang pu 06/2011; 31(6):1472-5. DOI:10.3964/j.issn.1000-0593(2011)06-1472-04 · 0.27 Impact Factor