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Nippon rinsho. Japanese journal of clinical medicine 07/2010; 68 Suppl 7:799-802.
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ABSTRACT: The functions of fukutin, a gene product responsible for Fukuyama type congenital muscular dystrophy, still remain unclear, although a relation to the glycosylation of alpha-dystroglycan is presumed. To investigate the functions of fukutin, immunohistochemistry, examination using cultured astrocytes, enzyme-linked immunosorbent assay (ELISA)-based binding assay and immunoprecipitation were performed using control muscle and central nervous system tissues. Immunohistochemistry showed that alpha-dystroglycan and fukutin were co-expressed, especially in the glial cytoplasm and glia limitans of the central nervous system. An anti-fukutin antibody added to the culture medium did not bring about any changes in the astrocytes cultured on laminin-coated dishes. Together with the immunohistochemical results, the intracellular function of fukutin is considered. ELISA-based binding assay and immunoprecipitation may suggest the direct binding of fukutin and alpha-dystroglycan, at least in part. Fukutin seems to bind to both the hypoglycosylated and fully glycosylated form of alpha-dystroglycan, and seems bind to the core area rather than the sugar chain of alpha-dystroglycan. Fukutin may directly interact with alpha-dystroglycan during glycosylation, but further examinations are needed to confirm these details.
Neuroscience Research 01/2007; 56(4):391-9. · 2.25 Impact Factor
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Nippon rinsho. Japanese journal of clinical medicine 09/2005; 63 Suppl 8:762-4.
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ABSTRACT: Because accumulation of oxidative modification products seems to relate to aging and has not been fully studied in fetal brains, an immunohistochemical examination was performed on nine brains ranging from 22-40 weeks of gestation. These brains did not demonstrate lesions except hypoxic-ischemic changes. Advanced glycation end products and 4-hydroxynonenal are generally reported to be negative in neurons of normal young brains, but, in the present study, distinct positive immunoreaction was observed in neurons of fetal brains. Positive immunoreaction appeared earlier in the medulla oblongata than in the cerebrum, and 4-hydroxynonenal began to accumulate earlier than advanced glycation end products. As for glial cells, advanced glycation end products and 4-hydroxynonenal were positive in reactive astrocytes in mid- to late gestation. Because hypoxic-ischemic changes were observed in most of the patients, it is possible that oxidative stress caused by hypoxic-ischemic may be involved in the accumulation of these products in the fetal brain. 8-Hydroxy-2'-deoxyguanosine was negative even in patients demonstrating positive reaction for advanced glycation end products and 4-hydroxynonenal. In the fetal brain, DNA might be strongly protected from oxidative damage. 4-Hydroxynonenal is generally positive in the cytoplasm but was positive in the nucleus of immature neurons and glial cells in the present study, suggesting a unique metabolism of the fetal brain.
Pediatric Neurology 03/2002; 26(2):116-22. · 1.52 Impact Factor
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ABSTRACT: To elucidate the expression of theMDR1 gene products P-glycoprotein (Pgp) in endothelial cells on newly formed blood microvessels in brain tumors, 30 brain tumors
were examined by immunohistochemistry using an anti-Pgp monoclonal antibody, JSB-1. Positive reactions for JSB-1 were detected
in endothelial cells in newly formed microvessels in all 16 cases of glioma but not in the 4 meningiomas. Although endothelial
cells in newly formed microvessels of all 10 metastatic carcinomas showed positive reactions, negative reactions were seen
in those of the primary carcinomas. Compared with reactions of the endothelial cells of normal cerebral capillaries, weak
reactions were found in the endothelial cells forming glomeruloid proliferation in newly formed microvessels in the eight
glioblastomas and at the border of the surrounding cerebral tissue of the metastatic carcinomas. Since the endothelial cells
showing glomeruloid proliferation also had a high proliferative cell nuclear antigen labeling index, the present findings
demonstrate a negative relationship between positive reactions for Pgp and the proliferative activities of endothelial cells
in cerebral capillaries.
Brain Tumor Pathology 04/1999; 16(1):23-27. · 1.19 Impact Factor
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[show abstract]
[hide abstract]
ABSTRACT: The functions of fukutin, a gene product responsible for Fukuyama type congenital muscular dystrophy, still remain unclear, although a relation to the glycosylation of α-dystroglycan is presumed. To investigate the functions of fukutin, immunohistochemistry, examination using cultured astrocytes, enzyme-linked immunosorbent assay (ELISA)-based binding assay and immunoprecipitation were performed using control muscle and central nervous system tissues. Immunohistochemistry showed that α-dystroglycan and fukutin were co-expressed, especially in the glial cytoplasm and glia limitans of the central nervous system. An anti-fukutin antibody added to the culture medium did not bring about any changes in the astrocytes cultured on laminin-coated dishes. Together with the immunohistochemical results, the intracellular function of fukutin is considered. ELISA-based binding assay and immunoprecipitation may suggest the direct binding of fukutin and α-dystroglycan, at least in part. Fukutin seems to bind to both the hypoglycosylated and fully glycosylated form of α-dystroglycan, and seems bind to the core area rather than the sugar chain of α-dystroglycan. Fukutin may directly interact with α-dystroglycan during glycosylation, but further examinations are needed to confirm these details.
Neuroscience Research.
